Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells

Detalhes bibliográficos
Autor(a) principal: Nunes,V.A.
Data de Publicação: 2003
Outros Autores: Gozzo,A.J., Juliano,M.A., Cerqueira César,M., Sampaio,M.U., Sampaio,C.A.M., Araújo,M.S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800010
Resumo: Apoptosis and necrosis are two distinct forms of cell death that can occur in response to different agents and stress conditions. In order to verify if the oxidative stress induced by dietary selenium and vitamin E deficiencies can lead muscle cells to apoptosis, one-day-old chicks were reared using diets differing in their vitamin E (0 or 10 IU/kg) and selenium (0 or 0.15 ppm) supplementation. Chick skeletal muscle tissue was obtained from 28-day-old animals and used to verify apoptosis occurrence based on caspase activity detection and DNA fragmentation. Antioxidant deficiency significantly increased caspase-like activity assessed by the hydrolysis of fluorogenic peptide substrates (Abz-peptidyl-EDDnp) at lambdaexc = 320 nm and lambdaem = 420 nm. Proteolytic activation was not accompanied by typical internucleosomal DNA fragmentation detected by field inversion gel electrophoresis. Although the general caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone (Z-VAD-fmk) (0 to 80 muM) did not block caspase-like activity when preincubated for 30 min with muscle homogenates, the hydrolyzed substrates presented the same cleavage profile in HPLC (at the aspartic acid residue) when incubated with the purified recombinant enzyme caspase-3. These data indicate that oxidative stress causes caspase-like activation in muscle cells and suggest that cell death associated with exudative diathesis (dietary deficiency of selenium and vitamin E) can follow the apoptotic pathway.
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spelling Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cellsOxidative stressCaspase activationApoptosisDietary selenium deficiencyDietary vitamin E deficiencyInternucleosomal DNA fragmentationCaspase substrateApoptosis and necrosis are two distinct forms of cell death that can occur in response to different agents and stress conditions. In order to verify if the oxidative stress induced by dietary selenium and vitamin E deficiencies can lead muscle cells to apoptosis, one-day-old chicks were reared using diets differing in their vitamin E (0 or 10 IU/kg) and selenium (0 or 0.15 ppm) supplementation. Chick skeletal muscle tissue was obtained from 28-day-old animals and used to verify apoptosis occurrence based on caspase activity detection and DNA fragmentation. Antioxidant deficiency significantly increased caspase-like activity assessed by the hydrolysis of fluorogenic peptide substrates (Abz-peptidyl-EDDnp) at lambdaexc = 320 nm and lambdaem = 420 nm. Proteolytic activation was not accompanied by typical internucleosomal DNA fragmentation detected by field inversion gel electrophoresis. Although the general caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone (Z-VAD-fmk) (0 to 80 muM) did not block caspase-like activity when preincubated for 30 min with muscle homogenates, the hydrolyzed substrates presented the same cleavage profile in HPLC (at the aspartic acid residue) when incubated with the purified recombinant enzyme caspase-3. These data indicate that oxidative stress causes caspase-like activation in muscle cells and suggest that cell death associated with exudative diathesis (dietary deficiency of selenium and vitamin E) can follow the apoptotic pathway.Associação Brasileira de Divulgação Científica2003-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800010Brazilian Journal of Medical and Biological Research v.36 n.8 2003reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2003000800010info:eu-repo/semantics/openAccessNunes,V.A.Gozzo,A.J.Juliano,M.A.Cerqueira César,M.Sampaio,M.U.Sampaio,C.A.M.Araújo,M.S.eng2003-07-23T00:00:00Zoai:scielo:S0100-879X2003000800010Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2003-07-23T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
title Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
spellingShingle Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
Nunes,V.A.
Oxidative stress
Caspase activation
Apoptosis
Dietary selenium deficiency
Dietary vitamin E deficiency
Internucleosomal DNA fragmentation
Caspase substrate
title_short Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
title_full Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
title_fullStr Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
title_full_unstemmed Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
title_sort Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
author Nunes,V.A.
author_facet Nunes,V.A.
Gozzo,A.J.
Juliano,M.A.
Cerqueira César,M.
Sampaio,M.U.
Sampaio,C.A.M.
Araújo,M.S.
author_role author
author2 Gozzo,A.J.
Juliano,M.A.
Cerqueira César,M.
Sampaio,M.U.
Sampaio,C.A.M.
Araújo,M.S.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nunes,V.A.
Gozzo,A.J.
Juliano,M.A.
Cerqueira César,M.
Sampaio,M.U.
Sampaio,C.A.M.
Araújo,M.S.
dc.subject.por.fl_str_mv Oxidative stress
Caspase activation
Apoptosis
Dietary selenium deficiency
Dietary vitamin E deficiency
Internucleosomal DNA fragmentation
Caspase substrate
topic Oxidative stress
Caspase activation
Apoptosis
Dietary selenium deficiency
Dietary vitamin E deficiency
Internucleosomal DNA fragmentation
Caspase substrate
description Apoptosis and necrosis are two distinct forms of cell death that can occur in response to different agents and stress conditions. In order to verify if the oxidative stress induced by dietary selenium and vitamin E deficiencies can lead muscle cells to apoptosis, one-day-old chicks were reared using diets differing in their vitamin E (0 or 10 IU/kg) and selenium (0 or 0.15 ppm) supplementation. Chick skeletal muscle tissue was obtained from 28-day-old animals and used to verify apoptosis occurrence based on caspase activity detection and DNA fragmentation. Antioxidant deficiency significantly increased caspase-like activity assessed by the hydrolysis of fluorogenic peptide substrates (Abz-peptidyl-EDDnp) at lambdaexc = 320 nm and lambdaem = 420 nm. Proteolytic activation was not accompanied by typical internucleosomal DNA fragmentation detected by field inversion gel electrophoresis. Although the general caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone (Z-VAD-fmk) (0 to 80 muM) did not block caspase-like activity when preincubated for 30 min with muscle homogenates, the hydrolyzed substrates presented the same cleavage profile in HPLC (at the aspartic acid residue) when incubated with the purified recombinant enzyme caspase-3. These data indicate that oxidative stress causes caspase-like activation in muscle cells and suggest that cell death associated with exudative diathesis (dietary deficiency of selenium and vitamin E) can follow the apoptotic pathway.
publishDate 2003
dc.date.none.fl_str_mv 2003-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2003000800010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.36 n.8 2003
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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