Immunologic patterns associated with cure in human American cutaneous leishmaniasis
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100019 |
Resumo: | Patients with American cutaneous leishmaniasis were studied before therapy (active lesion) and at the end of therapy (cured patients). Assays of lymphocyte proliferative responses of peripheral blood mononuclear cells induced in vitro by Leishmania braziliensis promastigote antigens (Lb) were performed. Antigen-stimulated cells were harvested for CD4 and CD8 phenotype analysis and the levels of gamma interferon (IFN-<FONT FACE="Symbol">g</font>) and interleukin 4 (IL-4) produced were also determined in the culture supernatants. Two different patterns of Lb-induced T cell responses were observed: a) predominance of responding CD4+ cells and mixed type 1 and type 2 cytokine production (IFN-<FONT FACE="Symbol">g</font> and IL-4) during the active disease, and b) similar proportions of responding CD4+ and CD8+ cells, and type 1 cytokine production (presence of IFN-<FONT FACE="Symbol">g</font> and very low IL-4) at the end of therapy (healed lesions). This last pattern is probably associated with a beneficial T cell response |
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Immunologic patterns associated with cure in human American cutaneous leishmaniasishuman cutaneous leishmaniasisLeishmania braziliensisCD4+ and CD8+ cellsIFN-<FONT FACE=Symbol>g</font>IL-4Patients with American cutaneous leishmaniasis were studied before therapy (active lesion) and at the end of therapy (cured patients). Assays of lymphocyte proliferative responses of peripheral blood mononuclear cells induced in vitro by Leishmania braziliensis promastigote antigens (Lb) were performed. Antigen-stimulated cells were harvested for CD4 and CD8 phenotype analysis and the levels of gamma interferon (IFN-<FONT FACE="Symbol">g</font>) and interleukin 4 (IL-4) produced were also determined in the culture supernatants. Two different patterns of Lb-induced T cell responses were observed: a) predominance of responding CD4+ cells and mixed type 1 and type 2 cytokine production (IFN-<FONT FACE="Symbol">g</font> and IL-4) during the active disease, and b) similar proportions of responding CD4+ and CD8+ cells, and type 1 cytokine production (presence of IFN-<FONT FACE="Symbol">g</font> and very low IL-4) at the end of therapy (healed lesions). This last pattern is probably associated with a beneficial T cell responseAssociação Brasileira de Divulgação Científica1998-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100019Brazilian Journal of Medical and Biological Research v.31 n.1 1998reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X1998000100019info:eu-repo/semantics/openAccessCoutinho,S.G.Da-Cruz,A.M.Bertho,A.L.Santiago,M.A.De-Luca,P.eng1998-10-07T00:00:00Zoai:scielo:S0100-879X1998000100019Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:1998-10-07T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Immunologic patterns associated with cure in human American cutaneous leishmaniasis |
title |
Immunologic patterns associated with cure in human American cutaneous leishmaniasis |
spellingShingle |
Immunologic patterns associated with cure in human American cutaneous leishmaniasis Coutinho,S.G. human cutaneous leishmaniasis Leishmania braziliensis CD4+ and CD8+ cells IFN-<FONT FACE=Symbol>g</font> IL-4 |
title_short |
Immunologic patterns associated with cure in human American cutaneous leishmaniasis |
title_full |
Immunologic patterns associated with cure in human American cutaneous leishmaniasis |
title_fullStr |
Immunologic patterns associated with cure in human American cutaneous leishmaniasis |
title_full_unstemmed |
Immunologic patterns associated with cure in human American cutaneous leishmaniasis |
title_sort |
Immunologic patterns associated with cure in human American cutaneous leishmaniasis |
author |
Coutinho,S.G. |
author_facet |
Coutinho,S.G. Da-Cruz,A.M. Bertho,A.L. Santiago,M.A. De-Luca,P. |
author_role |
author |
author2 |
Da-Cruz,A.M. Bertho,A.L. Santiago,M.A. De-Luca,P. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Coutinho,S.G. Da-Cruz,A.M. Bertho,A.L. Santiago,M.A. De-Luca,P. |
dc.subject.por.fl_str_mv |
human cutaneous leishmaniasis Leishmania braziliensis CD4+ and CD8+ cells IFN-<FONT FACE=Symbol>g</font> IL-4 |
topic |
human cutaneous leishmaniasis Leishmania braziliensis CD4+ and CD8+ cells IFN-<FONT FACE=Symbol>g</font> IL-4 |
description |
Patients with American cutaneous leishmaniasis were studied before therapy (active lesion) and at the end of therapy (cured patients). Assays of lymphocyte proliferative responses of peripheral blood mononuclear cells induced in vitro by Leishmania braziliensis promastigote antigens (Lb) were performed. Antigen-stimulated cells were harvested for CD4 and CD8 phenotype analysis and the levels of gamma interferon (IFN-<FONT FACE="Symbol">g</font>) and interleukin 4 (IL-4) produced were also determined in the culture supernatants. Two different patterns of Lb-induced T cell responses were observed: a) predominance of responding CD4+ cells and mixed type 1 and type 2 cytokine production (IFN-<FONT FACE="Symbol">g</font> and IL-4) during the active disease, and b) similar proportions of responding CD4+ and CD8+ cells, and type 1 cytokine production (presence of IFN-<FONT FACE="Symbol">g</font> and very low IL-4) at the end of therapy (healed lesions). This last pattern is probably associated with a beneficial T cell response |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-879X1998000100019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.31 n.1 1998 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302928929161216 |