Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells

Detalhes bibliográficos
Autor(a) principal: Merjan,A.J.
Data de Publicação: 2001
Outros Autores: Kanashiro,C.A., Krieger,J.E., Han,S.W., Paiva,A.C.M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900011
Resumo: A construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization.
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spelling Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cellsangiotensin IIagonist-induced receptor internalizationendocytosisfluorescence-tagged receptornuclear angiotensin receptorsrenin-angiotensin systemA construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization.Associação Brasileira de Divulgação Científica2001-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900011Brazilian Journal of Medical and Biological Research v.34 n.9 2001reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2001000900011info:eu-repo/semantics/openAccessMerjan,A.J.Kanashiro,C.A.Krieger,J.E.Han,S.W.Paiva,A.C.M.eng2001-08-16T00:00:00Zoai:scielo:S0100-879X2001000900011Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2001-08-16T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
title Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
spellingShingle Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
Merjan,A.J.
angiotensin II
agonist-induced receptor internalization
endocytosis
fluorescence-tagged receptor
nuclear angiotensin receptors
renin-angiotensin system
title_short Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
title_full Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
title_fullStr Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
title_full_unstemmed Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
title_sort Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
author Merjan,A.J.
author_facet Merjan,A.J.
Kanashiro,C.A.
Krieger,J.E.
Han,S.W.
Paiva,A.C.M.
author_role author
author2 Kanashiro,C.A.
Krieger,J.E.
Han,S.W.
Paiva,A.C.M.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Merjan,A.J.
Kanashiro,C.A.
Krieger,J.E.
Han,S.W.
Paiva,A.C.M.
dc.subject.por.fl_str_mv angiotensin II
agonist-induced receptor internalization
endocytosis
fluorescence-tagged receptor
nuclear angiotensin receptors
renin-angiotensin system
topic angiotensin II
agonist-induced receptor internalization
endocytosis
fluorescence-tagged receptor
nuclear angiotensin receptors
renin-angiotensin system
description A construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization.
publishDate 2001
dc.date.none.fl_str_mv 2001-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2001000900011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.34 n.9 2001
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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