Expression of Saccharomyces cerevisiae α-glucoside transporters under different growth conditions
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322014000100001 |
Resumo: | Important biotechnological processes depend on the efficient fermentation by Saccharomyces cerevisiae yeasts of starch hydrolysates rich in maltose and maltotriose. The rate-limiting step for fermentation of these α-glucosides is the transport across the plasma membrane of the cells. In order to contribute to a better understanding of maltose and maltotriose metabolism by S. cerevisiae, we analyzed the expression of the main α glucoside transporter genes in two different yeast strains grown on media with glucose, maltose or maltotriose as carbon source. Although both yeast strains have higher αglucoside transport activity during growth on maltotriose, our results show similar expression levels of the analyzed genes on either maltose or maltrotriose media. Thus, our results indicate that, although the transport capacity of maltotriose grown cells is higher than that of maltose grown cells, maltotriose cannot be considered a better inducer of αglucoside transporter genes. |
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Brazilian Journal of Chemical Engineering |
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Expression of Saccharomyces cerevisiae α-glucoside transporters under different growth conditionsExpressionTransportersMAL genesMaltoseMaltotrioseS. cerevisiaeImportant biotechnological processes depend on the efficient fermentation by Saccharomyces cerevisiae yeasts of starch hydrolysates rich in maltose and maltotriose. The rate-limiting step for fermentation of these α-glucosides is the transport across the plasma membrane of the cells. In order to contribute to a better understanding of maltose and maltotriose metabolism by S. cerevisiae, we analyzed the expression of the main α glucoside transporter genes in two different yeast strains grown on media with glucose, maltose or maltotriose as carbon source. Although both yeast strains have higher αglucoside transport activity during growth on maltotriose, our results show similar expression levels of the analyzed genes on either maltose or maltrotriose media. Thus, our results indicate that, although the transport capacity of maltotriose grown cells is higher than that of maltose grown cells, maltotriose cannot be considered a better inducer of αglucoside transporter genes.Brazilian Society of Chemical Engineering2014-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322014000100001Brazilian Journal of Chemical Engineering v.31 n.1 2014reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322014000100001info:eu-repo/semantics/openAccessAlves Jr.,S. L.Thevelein,J. M.Stambuk,B. U.eng2014-03-20T00:00:00Zoai:scielo:S0104-66322014000100001Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2014-03-20T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
Expression of Saccharomyces cerevisiae α-glucoside transporters under different growth conditions |
title |
Expression of Saccharomyces cerevisiae α-glucoside transporters under different growth conditions |
spellingShingle |
Expression of Saccharomyces cerevisiae α-glucoside transporters under different growth conditions Alves Jr.,S. L. Expression Transporters MAL genes Maltose Maltotriose S. cerevisiae |
title_short |
Expression of Saccharomyces cerevisiae α-glucoside transporters under different growth conditions |
title_full |
Expression of Saccharomyces cerevisiae α-glucoside transporters under different growth conditions |
title_fullStr |
Expression of Saccharomyces cerevisiae α-glucoside transporters under different growth conditions |
title_full_unstemmed |
Expression of Saccharomyces cerevisiae α-glucoside transporters under different growth conditions |
title_sort |
Expression of Saccharomyces cerevisiae α-glucoside transporters under different growth conditions |
author |
Alves Jr.,S. L. |
author_facet |
Alves Jr.,S. L. Thevelein,J. M. Stambuk,B. U. |
author_role |
author |
author2 |
Thevelein,J. M. Stambuk,B. U. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Alves Jr.,S. L. Thevelein,J. M. Stambuk,B. U. |
dc.subject.por.fl_str_mv |
Expression Transporters MAL genes Maltose Maltotriose S. cerevisiae |
topic |
Expression Transporters MAL genes Maltose Maltotriose S. cerevisiae |
description |
Important biotechnological processes depend on the efficient fermentation by Saccharomyces cerevisiae yeasts of starch hydrolysates rich in maltose and maltotriose. The rate-limiting step for fermentation of these α-glucosides is the transport across the plasma membrane of the cells. In order to contribute to a better understanding of maltose and maltotriose metabolism by S. cerevisiae, we analyzed the expression of the main α glucoside transporter genes in two different yeast strains grown on media with glucose, maltose or maltotriose as carbon source. Although both yeast strains have higher αglucoside transport activity during growth on maltotriose, our results show similar expression levels of the analyzed genes on either maltose or maltrotriose media. Thus, our results indicate that, although the transport capacity of maltotriose grown cells is higher than that of maltose grown cells, maltotriose cannot be considered a better inducer of αglucoside transporter genes. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322014000100001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322014000100001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0104-66322014000100001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.31 n.1 2014 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213174245064704 |