Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322012000400002 |
Resumo: | The nutritional medium compositions required for chitosanase production by Streptomyces albus YT2 were optimized, and the strain was isolated from the coastal soil of Bohai Sea. Plackett-Burman design was applied to evaluate the effect of different medium compositions obtained by one-factor-at-a-time experiments. Glucose, peptone and MgSO4 were found to influence the activity of chitosanase significantly. The steepest ascent method was employed to approach the experimental design space, followed by the use of Box-Behnken design and response surface methodology for further optimization of the chitosanase production. The medium composition for optimal production of chitosanase was as follows: 0.5 g/L chitosan, 55 g/L glucose, 1 g/L peptone, 2 g/L (NH4)2SO4, 8 g/L urea, 1 g/L NH4Cl, 3 g/L KH2PO4, 1 g/L FeSO4·7H2O, 2 g/L ZnSO4·7H2O, 2 g/L CaCl2·6H2O, 2 g/L MnSO4·H2O and 2 g/L NaCl. The chitosanase production was found to increase from 11.56 U/mL to 39.87 U/mL, a 3.47-fold increase compared with that using the original medium. |
id |
ABEQ-1_e3da4a6d4b1b84e8108782ee6a126da4 |
---|---|
oai_identifier_str |
oai:scielo:S0104-66322012000400002 |
network_acronym_str |
ABEQ-1 |
network_name_str |
Brazilian Journal of Chemical Engineering |
repository_id_str |
|
spelling |
Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albusChitosanaseFermentation optimizationMedium compositionsResponse surface methodologyStreptomyces albusThe nutritional medium compositions required for chitosanase production by Streptomyces albus YT2 were optimized, and the strain was isolated from the coastal soil of Bohai Sea. Plackett-Burman design was applied to evaluate the effect of different medium compositions obtained by one-factor-at-a-time experiments. Glucose, peptone and MgSO4 were found to influence the activity of chitosanase significantly. The steepest ascent method was employed to approach the experimental design space, followed by the use of Box-Behnken design and response surface methodology for further optimization of the chitosanase production. The medium composition for optimal production of chitosanase was as follows: 0.5 g/L chitosan, 55 g/L glucose, 1 g/L peptone, 2 g/L (NH4)2SO4, 8 g/L urea, 1 g/L NH4Cl, 3 g/L KH2PO4, 1 g/L FeSO4·7H2O, 2 g/L ZnSO4·7H2O, 2 g/L CaCl2·6H2O, 2 g/L MnSO4·H2O and 2 g/L NaCl. The chitosanase production was found to increase from 11.56 U/mL to 39.87 U/mL, a 3.47-fold increase compared with that using the original medium.Brazilian Society of Chemical Engineering2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322012000400002Brazilian Journal of Chemical Engineering v.29 n.4 2012reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322012000400002info:eu-repo/semantics/openAccessCheng,S. W.Wang,Y. F.Hong,B.eng2013-01-03T00:00:00Zoai:scielo:S0104-66322012000400002Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2013-01-03T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus |
title |
Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus |
spellingShingle |
Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus Cheng,S. W. Chitosanase Fermentation optimization Medium compositions Response surface methodology Streptomyces albus |
title_short |
Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus |
title_full |
Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus |
title_fullStr |
Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus |
title_full_unstemmed |
Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus |
title_sort |
Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus |
author |
Cheng,S. W. |
author_facet |
Cheng,S. W. Wang,Y. F. Hong,B. |
author_role |
author |
author2 |
Wang,Y. F. Hong,B. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Cheng,S. W. Wang,Y. F. Hong,B. |
dc.subject.por.fl_str_mv |
Chitosanase Fermentation optimization Medium compositions Response surface methodology Streptomyces albus |
topic |
Chitosanase Fermentation optimization Medium compositions Response surface methodology Streptomyces albus |
description |
The nutritional medium compositions required for chitosanase production by Streptomyces albus YT2 were optimized, and the strain was isolated from the coastal soil of Bohai Sea. Plackett-Burman design was applied to evaluate the effect of different medium compositions obtained by one-factor-at-a-time experiments. Glucose, peptone and MgSO4 were found to influence the activity of chitosanase significantly. The steepest ascent method was employed to approach the experimental design space, followed by the use of Box-Behnken design and response surface methodology for further optimization of the chitosanase production. The medium composition for optimal production of chitosanase was as follows: 0.5 g/L chitosan, 55 g/L glucose, 1 g/L peptone, 2 g/L (NH4)2SO4, 8 g/L urea, 1 g/L NH4Cl, 3 g/L KH2PO4, 1 g/L FeSO4·7H2O, 2 g/L ZnSO4·7H2O, 2 g/L CaCl2·6H2O, 2 g/L MnSO4·H2O and 2 g/L NaCl. The chitosanase production was found to increase from 11.56 U/mL to 39.87 U/mL, a 3.47-fold increase compared with that using the original medium. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322012000400002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322012000400002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0104-66322012000400002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.29 n.4 2012 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213173853945856 |