Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of Seed Science |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372019000100007 |
Resumo: | Abstract: Quick tests are essential tools to evaluate seed quality. The objective of this study was to adapt the methodology of tetrazolium test, by identifying the most suitable pre-conditioning parameters of imbibition temperature and duration, and tetrazolium solution concentrations, in order to assess the physiological quality of chickpea seeds. Also, this work proposed the separation of lots in classes, according to the viability and vigor of the seeds. Three lots of chickpeas (lot 1 - BRS Aleppo C1, lot 2 - BRS Aleppo basic, and lot 3 - Cicero) were evaluated. They were analyzed according to three combinations of imbibition temperature and duration (41 °C for 4 hours, 41 ºC for 6 hours, and 30 ºC for 18 hours) and two tetrazolium concentrations (0.1% and 0.5%). The imbibition at 41 °C for 4 or 6 hours, and at 30 ºC for 18 hours allowed clear visualization of injuries in the seeds after immersion in 0.1% tetrazolium solution. The intense coloration formed by the 0.5% solution of this salt prevented the differentiation of the types of damage. In addition, it was possible to separate the seeds into four classes: class I (viable and vigorous, without damages), class II (viable and vigorous, with superficial injuries), class III (viable and non-vigorous), and class IV (non-viable). |
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Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seedsCicer arietinumseed analysisviabilityvigorAbstract: Quick tests are essential tools to evaluate seed quality. The objective of this study was to adapt the methodology of tetrazolium test, by identifying the most suitable pre-conditioning parameters of imbibition temperature and duration, and tetrazolium solution concentrations, in order to assess the physiological quality of chickpea seeds. Also, this work proposed the separation of lots in classes, according to the viability and vigor of the seeds. Three lots of chickpeas (lot 1 - BRS Aleppo C1, lot 2 - BRS Aleppo basic, and lot 3 - Cicero) were evaluated. They were analyzed according to three combinations of imbibition temperature and duration (41 °C for 4 hours, 41 ºC for 6 hours, and 30 ºC for 18 hours) and two tetrazolium concentrations (0.1% and 0.5%). The imbibition at 41 °C for 4 or 6 hours, and at 30 ºC for 18 hours allowed clear visualization of injuries in the seeds after immersion in 0.1% tetrazolium solution. The intense coloration formed by the 0.5% solution of this salt prevented the differentiation of the types of damage. In addition, it was possible to separate the seeds into four classes: class I (viable and vigorous, without damages), class II (viable and vigorous, with superficial injuries), class III (viable and non-vigorous), and class IV (non-viable).ABRATES - Associação Brasileira de Tecnologia de Sementes2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372019000100007Journal of Seed Science v.41 n.1 2019reponame:Journal of Seed Scienceinstname:Associação Brasileira de Tecnologia de Sementes (ABRATES)instacron:ABRATES10.1590/2317-1545v41n1187777info:eu-repo/semantics/openAccessParaíso,Humberto AlencarBrandão Junior,Delacyr da SilvaAvelar,Ramon Ivo SoaresCosta,Candido Alves daGomes,Luan Souza de PaulaNascimento,Warley Marcoseng2019-03-25T00:00:00Zoai:scielo:S2317-15372019000100007Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=2317-1537&lng=en&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||abrates@abrates.org.br2317-15452317-1537opendoar:2019-03-25T00:00Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES)false |
dc.title.none.fl_str_mv |
Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds |
title |
Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds |
spellingShingle |
Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds Paraíso,Humberto Alencar Cicer arietinum seed analysis viability vigor |
title_short |
Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds |
title_full |
Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds |
title_fullStr |
Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds |
title_full_unstemmed |
Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds |
title_sort |
Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds |
author |
Paraíso,Humberto Alencar |
author_facet |
Paraíso,Humberto Alencar Brandão Junior,Delacyr da Silva Avelar,Ramon Ivo Soares Costa,Candido Alves da Gomes,Luan Souza de Paula Nascimento,Warley Marcos |
author_role |
author |
author2 |
Brandão Junior,Delacyr da Silva Avelar,Ramon Ivo Soares Costa,Candido Alves da Gomes,Luan Souza de Paula Nascimento,Warley Marcos |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Paraíso,Humberto Alencar Brandão Junior,Delacyr da Silva Avelar,Ramon Ivo Soares Costa,Candido Alves da Gomes,Luan Souza de Paula Nascimento,Warley Marcos |
dc.subject.por.fl_str_mv |
Cicer arietinum seed analysis viability vigor |
topic |
Cicer arietinum seed analysis viability vigor |
description |
Abstract: Quick tests are essential tools to evaluate seed quality. The objective of this study was to adapt the methodology of tetrazolium test, by identifying the most suitable pre-conditioning parameters of imbibition temperature and duration, and tetrazolium solution concentrations, in order to assess the physiological quality of chickpea seeds. Also, this work proposed the separation of lots in classes, according to the viability and vigor of the seeds. Three lots of chickpeas (lot 1 - BRS Aleppo C1, lot 2 - BRS Aleppo basic, and lot 3 - Cicero) were evaluated. They were analyzed according to three combinations of imbibition temperature and duration (41 °C for 4 hours, 41 ºC for 6 hours, and 30 ºC for 18 hours) and two tetrazolium concentrations (0.1% and 0.5%). The imbibition at 41 °C for 4 or 6 hours, and at 30 ºC for 18 hours allowed clear visualization of injuries in the seeds after immersion in 0.1% tetrazolium solution. The intense coloration formed by the 0.5% solution of this salt prevented the differentiation of the types of damage. In addition, it was possible to separate the seeds into four classes: class I (viable and vigorous, without damages), class II (viable and vigorous, with superficial injuries), class III (viable and non-vigorous), and class IV (non-viable). |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372019000100007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372019000100007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/2317-1545v41n1187777 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
ABRATES - Associação Brasileira de Tecnologia de Sementes |
publisher.none.fl_str_mv |
ABRATES - Associação Brasileira de Tecnologia de Sementes |
dc.source.none.fl_str_mv |
Journal of Seed Science v.41 n.1 2019 reponame:Journal of Seed Science instname:Associação Brasileira de Tecnologia de Sementes (ABRATES) instacron:ABRATES |
instname_str |
Associação Brasileira de Tecnologia de Sementes (ABRATES) |
instacron_str |
ABRATES |
institution |
ABRATES |
reponame_str |
Journal of Seed Science |
collection |
Journal of Seed Science |
repository.name.fl_str_mv |
Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES) |
repository.mail.fl_str_mv |
||abrates@abrates.org.br |
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1754212982846390272 |