Using Tomato Analyzer software to determine embryo size in x-rayed seeds

• Autor(a) principal: Marcos Filho,Julio
• Data de Publicação: 2010
• Outros Autores: Gomes Junior,Francisco Guilhien, Bennett,Mark A, Wells,Arthur A, Stieve,Susan
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista Brasileira de Sementes (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222010000200018
• topic: cotton; cucurbits; imaging analysis; X-ray

A primary interest of image analysis of X-rayed seeds is to identify whether the extent of fill in the embryo cavity is associated with to seed physiological quality. The objective of this research was to verify the accuracy of the freely available Tomato Analyzer (TA) software developed at The Ohio State University to determine the ratio of embryo size over total seed area. Seeds of pumpkin, watermelon, cucumber and cotton were X-rayed and analyzed by the software which defines seed and embryo boundaries and automatically generates numerical values to quantify that ratio. Results showed that the TA has the sensitivity to evaluate the extent of embryo growth within the cucurbits and cotton seeds and is a promising alternative for this assessment in other seed species.