Detection of blaOXA-23 in Acinetobacter spp. isolated from patients of a university hospital
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Infectious Diseases |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600004 |
Resumo: | INTRODUCTION: Acinetobacter spp. have emerged as notorious pathogens involved in healthcareassociated infections. Carbapenems are important antimicrobial agents for treating infections due to multidrug resistant Acinetobacter spp. Different mechanisms may confer resistance to these drugs in the genus, particularly production of class D carbapenemases. OXA-23-like family has been pointed out as one of the predominant carbapenamases among Acinetobacter. The present work aimed to investigate the occurrence of OXA-23-like carbapenemases among Acinetobacter isolates recovered from patients of a university hospital in Niterói, RJ, Brazil. METHODS: Antimicrobial susceptibility profiles were determined by disk-diffusion. Imipenem resistant isolates were submitted to Modified Hodge Test in order to screen for carbapenemase production, and later to polymerase chain reaction (PCR) to investigate the presence of blaOXA-23. RESULTS: Imipenem and meropenem resistance rates were 71.4% and 69.7%, respectively. The Modified Hodge Test revealed carbapenemase production among 76 (89.4%) of the 85 imipenem resistant isolates analyzed; according to PCR results, 81 isolates (95.4%) carried the blaOXA-23 gene. CONCLUSIONS: OXA-23-like enzymes may be an important mechanism of carbapenem resistance among isolates present in the hospital studied. |
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Brazilian Journal of Infectious Diseases |
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Detection of blaOXA-23 in Acinetobacter spp. isolated from patients of a university hospitalAcinetobacter sppOXA-23Carbapenem resistanceINTRODUCTION: Acinetobacter spp. have emerged as notorious pathogens involved in healthcareassociated infections. Carbapenems are important antimicrobial agents for treating infections due to multidrug resistant Acinetobacter spp. Different mechanisms may confer resistance to these drugs in the genus, particularly production of class D carbapenemases. OXA-23-like family has been pointed out as one of the predominant carbapenamases among Acinetobacter. The present work aimed to investigate the occurrence of OXA-23-like carbapenemases among Acinetobacter isolates recovered from patients of a university hospital in Niterói, RJ, Brazil. METHODS: Antimicrobial susceptibility profiles were determined by disk-diffusion. Imipenem resistant isolates were submitted to Modified Hodge Test in order to screen for carbapenemase production, and later to polymerase chain reaction (PCR) to investigate the presence of blaOXA-23. RESULTS: Imipenem and meropenem resistance rates were 71.4% and 69.7%, respectively. The Modified Hodge Test revealed carbapenemase production among 76 (89.4%) of the 85 imipenem resistant isolates analyzed; according to PCR results, 81 isolates (95.4%) carried the blaOXA-23 gene. CONCLUSIONS: OXA-23-like enzymes may be an important mechanism of carbapenem resistance among isolates present in the hospital studied.Brazilian Society of Infectious Diseases2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600004Brazilian Journal of Infectious Diseases v.16 n.6 2012reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1016/j.bjid.2012.10.003info:eu-repo/semantics/openAccessCorrêa,Laís LisboaBotelho,Larissa Alvarenga BatistaBarbosa,Lívia CarvalhoMattos,Claudio SimõesCarballido,Jupira MironCastro,Carmem Lúcia Teixeira deMondino,Pedro Juan JosePaula,Geraldo Renato deMondino,Silvia Susana Bona deMendonça-Souza,Claudia Rezende Vieira deeng2013-02-20T00:00:00Zoai:scielo:S1413-86702012000600004Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2013-02-20T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false |
dc.title.none.fl_str_mv |
Detection of blaOXA-23 in Acinetobacter spp. isolated from patients of a university hospital |
title |
Detection of blaOXA-23 in Acinetobacter spp. isolated from patients of a university hospital |
spellingShingle |
Detection of blaOXA-23 in Acinetobacter spp. isolated from patients of a university hospital Corrêa,Laís Lisboa Acinetobacter spp OXA-23 Carbapenem resistance |
title_short |
Detection of blaOXA-23 in Acinetobacter spp. isolated from patients of a university hospital |
title_full |
Detection of blaOXA-23 in Acinetobacter spp. isolated from patients of a university hospital |
title_fullStr |
Detection of blaOXA-23 in Acinetobacter spp. isolated from patients of a university hospital |
title_full_unstemmed |
Detection of blaOXA-23 in Acinetobacter spp. isolated from patients of a university hospital |
title_sort |
Detection of blaOXA-23 in Acinetobacter spp. isolated from patients of a university hospital |
author |
Corrêa,Laís Lisboa |
author_facet |
Corrêa,Laís Lisboa Botelho,Larissa Alvarenga Batista Barbosa,Lívia Carvalho Mattos,Claudio Simões Carballido,Jupira Miron Castro,Carmem Lúcia Teixeira de Mondino,Pedro Juan Jose Paula,Geraldo Renato de Mondino,Silvia Susana Bona de Mendonça-Souza,Claudia Rezende Vieira de |
author_role |
author |
author2 |
Botelho,Larissa Alvarenga Batista Barbosa,Lívia Carvalho Mattos,Claudio Simões Carballido,Jupira Miron Castro,Carmem Lúcia Teixeira de Mondino,Pedro Juan Jose Paula,Geraldo Renato de Mondino,Silvia Susana Bona de Mendonça-Souza,Claudia Rezende Vieira de |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Corrêa,Laís Lisboa Botelho,Larissa Alvarenga Batista Barbosa,Lívia Carvalho Mattos,Claudio Simões Carballido,Jupira Miron Castro,Carmem Lúcia Teixeira de Mondino,Pedro Juan Jose Paula,Geraldo Renato de Mondino,Silvia Susana Bona de Mendonça-Souza,Claudia Rezende Vieira de |
dc.subject.por.fl_str_mv |
Acinetobacter spp OXA-23 Carbapenem resistance |
topic |
Acinetobacter spp OXA-23 Carbapenem resistance |
description |
INTRODUCTION: Acinetobacter spp. have emerged as notorious pathogens involved in healthcareassociated infections. Carbapenems are important antimicrobial agents for treating infections due to multidrug resistant Acinetobacter spp. Different mechanisms may confer resistance to these drugs in the genus, particularly production of class D carbapenemases. OXA-23-like family has been pointed out as one of the predominant carbapenamases among Acinetobacter. The present work aimed to investigate the occurrence of OXA-23-like carbapenemases among Acinetobacter isolates recovered from patients of a university hospital in Niterói, RJ, Brazil. METHODS: Antimicrobial susceptibility profiles were determined by disk-diffusion. Imipenem resistant isolates were submitted to Modified Hodge Test in order to screen for carbapenemase production, and later to polymerase chain reaction (PCR) to investigate the presence of blaOXA-23. RESULTS: Imipenem and meropenem resistance rates were 71.4% and 69.7%, respectively. The Modified Hodge Test revealed carbapenemase production among 76 (89.4%) of the 85 imipenem resistant isolates analyzed; according to PCR results, 81 isolates (95.4%) carried the blaOXA-23 gene. CONCLUSIONS: OXA-23-like enzymes may be an important mechanism of carbapenem resistance among isolates present in the hospital studied. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjid.2012.10.003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
dc.source.none.fl_str_mv |
Brazilian Journal of Infectious Diseases v.16 n.6 2012 reponame:Brazilian Journal of Infectious Diseases instname:Brazilian Society of Infectious Diseases (BSID) instacron:BSID |
instname_str |
Brazilian Society of Infectious Diseases (BSID) |
instacron_str |
BSID |
institution |
BSID |
reponame_str |
Brazilian Journal of Infectious Diseases |
collection |
Brazilian Journal of Infectious Diseases |
repository.name.fl_str_mv |
Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID) |
repository.mail.fl_str_mv |
bjid@bjid.org.br||lgoldani@ufrgs.br |
_version_ |
1754209242371325952 |