Direct visualization of the action of triton X-100 on giant vesicles of erythrocyte membrane lipids
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Título da fonte: | Repositório da Produção Científica e Intelectual da Unicamp |
Texto Completo: | https://hdl.handle.net/20.500.12733/471 |
Resumo: | Abstract: The raft hypothesis proposes that microdomains enriched in sphingolipids, cholesterol, and specific proteins are transiently formed to accomplish important cellular tasks. Equivocally, detergent-resistant membranes were initially assumed to be identical to membrane rafts, because of similarities between their compositions. In fact, the impact of detergents in membrane organization is still controversial. Here, we use phase contrast and fluorescence microscopy to observe giant unilamellar vesicles (GUVs) made of erythrocyte membrane lipids (erythro-GUVs) when exposed to the detergent Triton X-100 (TX-100). We clearly show that TX-100 has a restructuring action on biomembranes. Contact with TX-100 readily induces domain formation on the previously homogeneous membrane of erythro-GUVs at physiological and room temperatures. The shape and dynamics of the formed domains point to liquid-ordered/liquid-disordered (Lo/Ld) phase separation, typically found in raft-like ternary lipid mixtures. The Ld domains are then separated from the original vesicle and completely solubilized by TX-100. The insoluble vesicle left, in the Lo phase, represents around 2/3 of the original vesicle surface at room temperature and decreases to almost 1/2 at physiological temperature. This chain of events could be entirely reproduced with biomimetic GUVs of a simple ternary lipid mixture, 2:1:2 POPC/SM/chol (phosphatidylcholine/sphyngomyelin/cholesterol), showing that this behavior will arise because of fundamental physicochemical properties of simple lipid mixtures. This work provides direct visualization of TX-100-induced domain formation followed by selective (Ld phase) solubilization in a model system with a complex biological lipid composition |
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Direct visualization of the action of triton X-100 on giant vesicles of erythrocyte membrane lipidsOctoxinolOctoxynolErythrocyte membraneArtigo originalAbstract: The raft hypothesis proposes that microdomains enriched in sphingolipids, cholesterol, and specific proteins are transiently formed to accomplish important cellular tasks. Equivocally, detergent-resistant membranes were initially assumed to be identical to membrane rafts, because of similarities between their compositions. In fact, the impact of detergents in membrane organization is still controversial. Here, we use phase contrast and fluorescence microscopy to observe giant unilamellar vesicles (GUVs) made of erythrocyte membrane lipids (erythro-GUVs) when exposed to the detergent Triton X-100 (TX-100). We clearly show that TX-100 has a restructuring action on biomembranes. Contact with TX-100 readily induces domain formation on the previously homogeneous membrane of erythro-GUVs at physiological and room temperatures. The shape and dynamics of the formed domains point to liquid-ordered/liquid-disordered (Lo/Ld) phase separation, typically found in raft-like ternary lipid mixtures. The Ld domains are then separated from the original vesicle and completely solubilized by TX-100. The insoluble vesicle left, in the Lo phase, represents around 2/3 of the original vesicle surface at room temperature and decreases to almost 1/2 at physiological temperature. This chain of events could be entirely reproduced with biomimetic GUVs of a simple ternary lipid mixture, 2:1:2 POPC/SM/chol (phosphatidylcholine/sphyngomyelin/cholesterol), showing that this behavior will arise because of fundamental physicochemical properties of simple lipid mixtures. This work provides direct visualization of TX-100-induced domain formation followed by selective (Ld phase) solubilization in a model system with a complex biological lipid compositionCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPFechadoUNIVERSIDADE ESTADUAL DE CAMPINASCasadei, Bruna Renata, 1986-Domingues, Cleyton Crepaldi, 1975-Paula, Eneida de, 1963-2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12733/471CASADEI, Bruna Renata; DOMINGUES, Cleyton Crepaldi; PAULA, Eneida de. Direct visualization of the action of triton X-100 on giant vesicles of erythrocyte membrane lipids. Biophysical journal. St. Louis, MO : Cell, 2014. Vol. 106, n. 11 (2014), p. 2417-2425. Disponível em: https://hdl.handle.net/20.500.12733/471. Acesso em: 24 mai. 2023.Inglêshttps://repositorio.unicamp.br/acervo/detalhe/1231634reponame:Repositório da Produção Científica e Intelectual da Unicampinstname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPinfo:eu-repo/semantics/openAccess2021-11-12T11:33:20Zoai:https://www.repositorio.unicamp.br/:1231634Repositório InstitucionalPUBhttp://repositorio.unicamp.br/oai/requestreposip@unicamp.bropendoar:2021-11-12T11:33:20Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP)false |
dc.title.none.fl_str_mv |
Direct visualization of the action of triton X-100 on giant vesicles of erythrocyte membrane lipids |
title |
Direct visualization of the action of triton X-100 on giant vesicles of erythrocyte membrane lipids |
spellingShingle |
Direct visualization of the action of triton X-100 on giant vesicles of erythrocyte membrane lipids Casadei, Bruna Renata, 1986- Octoxinol Octoxynol Erythrocyte membrane Artigo original |
title_short |
Direct visualization of the action of triton X-100 on giant vesicles of erythrocyte membrane lipids |
title_full |
Direct visualization of the action of triton X-100 on giant vesicles of erythrocyte membrane lipids |
title_fullStr |
Direct visualization of the action of triton X-100 on giant vesicles of erythrocyte membrane lipids |
title_full_unstemmed |
Direct visualization of the action of triton X-100 on giant vesicles of erythrocyte membrane lipids |
title_sort |
Direct visualization of the action of triton X-100 on giant vesicles of erythrocyte membrane lipids |
author |
Casadei, Bruna Renata, 1986- |
author_facet |
Casadei, Bruna Renata, 1986- Domingues, Cleyton Crepaldi, 1975- Paula, Eneida de, 1963- |
author_role |
author |
author2 |
Domingues, Cleyton Crepaldi, 1975- Paula, Eneida de, 1963- |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
UNIVERSIDADE ESTADUAL DE CAMPINAS |
dc.contributor.author.fl_str_mv |
Casadei, Bruna Renata, 1986- Domingues, Cleyton Crepaldi, 1975- Paula, Eneida de, 1963- |
dc.subject.por.fl_str_mv |
Octoxinol Octoxynol Erythrocyte membrane Artigo original |
topic |
Octoxinol Octoxynol Erythrocyte membrane Artigo original |
description |
Abstract: The raft hypothesis proposes that microdomains enriched in sphingolipids, cholesterol, and specific proteins are transiently formed to accomplish important cellular tasks. Equivocally, detergent-resistant membranes were initially assumed to be identical to membrane rafts, because of similarities between their compositions. In fact, the impact of detergents in membrane organization is still controversial. Here, we use phase contrast and fluorescence microscopy to observe giant unilamellar vesicles (GUVs) made of erythrocyte membrane lipids (erythro-GUVs) when exposed to the detergent Triton X-100 (TX-100). We clearly show that TX-100 has a restructuring action on biomembranes. Contact with TX-100 readily induces domain formation on the previously homogeneous membrane of erythro-GUVs at physiological and room temperatures. The shape and dynamics of the formed domains point to liquid-ordered/liquid-disordered (Lo/Ld) phase separation, typically found in raft-like ternary lipid mixtures. The Ld domains are then separated from the original vesicle and completely solubilized by TX-100. The insoluble vesicle left, in the Lo phase, represents around 2/3 of the original vesicle surface at room temperature and decreases to almost 1/2 at physiological temperature. This chain of events could be entirely reproduced with biomimetic GUVs of a simple ternary lipid mixture, 2:1:2 POPC/SM/chol (phosphatidylcholine/sphyngomyelin/cholesterol), showing that this behavior will arise because of fundamental physicochemical properties of simple lipid mixtures. This work provides direct visualization of TX-100-induced domain formation followed by selective (Ld phase) solubilization in a model system with a complex biological lipid composition |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/20.500.12733/471 CASADEI, Bruna Renata; DOMINGUES, Cleyton Crepaldi; PAULA, Eneida de. Direct visualization of the action of triton X-100 on giant vesicles of erythrocyte membrane lipids. Biophysical journal. St. Louis, MO : Cell, 2014. Vol. 106, n. 11 (2014), p. 2417-2425. Disponível em: https://hdl.handle.net/20.500.12733/471. Acesso em: 24 mai. 2023. |
url |
https://hdl.handle.net/20.500.12733/471 |
identifier_str_mv |
CASADEI, Bruna Renata; DOMINGUES, Cleyton Crepaldi; PAULA, Eneida de. Direct visualization of the action of triton X-100 on giant vesicles of erythrocyte membrane lipids. Biophysical journal. St. Louis, MO : Cell, 2014. Vol. 106, n. 11 (2014), p. 2417-2425. Disponível em: https://hdl.handle.net/20.500.12733/471. Acesso em: 24 mai. 2023. |
dc.language.iso.fl_str_mv |
Inglês |
language_invalid_str_mv |
Inglês |
dc.relation.none.fl_str_mv |
https://repositorio.unicamp.br/acervo/detalhe/1231634 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório da Produção Científica e Intelectual da Unicamp instname:Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP |
instname_str |
Universidade Estadual de Campinas (UNICAMP) |
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UNICAMP |
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UNICAMP |
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Repositório da Produção Científica e Intelectual da Unicamp |
collection |
Repositório da Produção Científica e Intelectual da Unicamp |
repository.name.fl_str_mv |
Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP) |
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reposip@unicamp.br |
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