Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping

Detalhes bibliográficos
Autor(a) principal: Bueno,Rafael Delmond
Data de Publicação: 2018
Outros Autores: God,Pedro Ivo Vieira Good, Prata,Isadora Oliveira, Pereira,Pedro Henrique Scarpelli, Teixeira,Arlindo Inês, Piovesan,Newton Deniz, Barros,Everaldo Gonçalves de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000300244
Resumo: Abstract The development of molecular markers is essential for improvement of soybean cultivars with modified fatty acid content. The objective of this study was to identify and validate SNP markers in candidate genes for fatty acid content in soybean. Six candidate genes (ARAF, PDAT, ABI3, FAD2-1b, FAD3B, and FAD3C) were selected. Alignment of gene sequences identified 25 SNPs and 3 INDELs. TSP primers were used to identify SNP alleles. 259 recombinant inbred lines (RILs) (FA22 / CD219) and 185 F2 progenies (A29 / Tucunaré) were tested for association of SNPs. An SNP for FAD3B was associated with variation in content of linoleic acid (R2 = 5.84%) and linolenic acid (R2 = 6.79%). In FAD3C, an SNP was associated with linoleic and linolenic acids (R2 of 9.21% and 18.51%, respectively). The ABI3 gene was associated with palmitic acid, with R2 = 5.41%. The SNP markers identified will be used in assisted selection for improvement of fatty acid content.
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spelling Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotypingLow linolenic acidMAS-marker-assisted selectionSNP - single nucleotide polymorphismAbstract The development of molecular markers is essential for improvement of soybean cultivars with modified fatty acid content. The objective of this study was to identify and validate SNP markers in candidate genes for fatty acid content in soybean. Six candidate genes (ARAF, PDAT, ABI3, FAD2-1b, FAD3B, and FAD3C) were selected. Alignment of gene sequences identified 25 SNPs and 3 INDELs. TSP primers were used to identify SNP alleles. 259 recombinant inbred lines (RILs) (FA22 / CD219) and 185 F2 progenies (A29 / Tucunaré) were tested for association of SNPs. An SNP for FAD3B was associated with variation in content of linoleic acid (R2 = 5.84%) and linolenic acid (R2 = 6.79%). In FAD3C, an SNP was associated with linoleic and linolenic acids (R2 of 9.21% and 18.51%, respectively). The ABI3 gene was associated with palmitic acid, with R2 = 5.41%. The SNP markers identified will be used in assisted selection for improvement of fatty acid content.Crop Breeding and Applied Biotechnology2018-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000300244Crop Breeding and Applied Biotechnology v.18 n.3 2018reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332018v18n3a37info:eu-repo/semantics/openAccessBueno,Rafael DelmondGod,Pedro Ivo Vieira GoodPrata,Isadora OliveiraPereira,Pedro Henrique ScarpelliTeixeira,Arlindo InêsPiovesan,Newton DenizBarros,Everaldo Gonçalves deeng2018-07-05T00:00:00Zoai:scielo:S1984-70332018000300244Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2018-07-05T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping
title Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping
spellingShingle Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping
Bueno,Rafael Delmond
Low linolenic acid
MAS-marker-assisted selection
SNP - single nucleotide polymorphism
title_short Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping
title_full Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping
title_fullStr Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping
title_full_unstemmed Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping
title_sort Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping
author Bueno,Rafael Delmond
author_facet Bueno,Rafael Delmond
God,Pedro Ivo Vieira Good
Prata,Isadora Oliveira
Pereira,Pedro Henrique Scarpelli
Teixeira,Arlindo Inês
Piovesan,Newton Deniz
Barros,Everaldo Gonçalves de
author_role author
author2 God,Pedro Ivo Vieira Good
Prata,Isadora Oliveira
Pereira,Pedro Henrique Scarpelli
Teixeira,Arlindo Inês
Piovesan,Newton Deniz
Barros,Everaldo Gonçalves de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bueno,Rafael Delmond
God,Pedro Ivo Vieira Good
Prata,Isadora Oliveira
Pereira,Pedro Henrique Scarpelli
Teixeira,Arlindo Inês
Piovesan,Newton Deniz
Barros,Everaldo Gonçalves de
dc.subject.por.fl_str_mv Low linolenic acid
MAS-marker-assisted selection
SNP - single nucleotide polymorphism
topic Low linolenic acid
MAS-marker-assisted selection
SNP - single nucleotide polymorphism
description Abstract The development of molecular markers is essential for improvement of soybean cultivars with modified fatty acid content. The objective of this study was to identify and validate SNP markers in candidate genes for fatty acid content in soybean. Six candidate genes (ARAF, PDAT, ABI3, FAD2-1b, FAD3B, and FAD3C) were selected. Alignment of gene sequences identified 25 SNPs and 3 INDELs. TSP primers were used to identify SNP alleles. 259 recombinant inbred lines (RILs) (FA22 / CD219) and 185 F2 progenies (A29 / Tucunaré) were tested for association of SNPs. An SNP for FAD3B was associated with variation in content of linoleic acid (R2 = 5.84%) and linolenic acid (R2 = 6.79%). In FAD3C, an SNP was associated with linoleic and linolenic acids (R2 of 9.21% and 18.51%, respectively). The ABI3 gene was associated with palmitic acid, with R2 = 5.41%. The SNP markers identified will be used in assisted selection for improvement of fatty acid content.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000300244
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000300244
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332018v18n3a37
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.18 n.3 2018
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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