Haploid identification using tropicalized haploid inducer progenies in maize
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Crop Breeding and Applied Biotechnology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000100016 |
Resumo: | Abstract The aim of this study was to identify maize haploid plants and compare the efficiency of identification of maize haploid plants using the R1-nj morphological marker, plant vigor, flow cytometry, chromosome counting, and microsatellite molecular markers under tropical conditions. We also established a protocol for chromosome duplication in maize haploid plants. Fourteen S0:1 and seven S2:3 haploid inducer progenies were crossed with GNZ9501 in 2012/2013 and 2014/2015, respectively. Through use of the R1-nj trait, we were able to identify 552 putative haploid seeds in 2012/2013 and 260 putative haploid seeds in 2014/2015. Only 1.84% were true positives according to flow cytometry in 2012/2013. In 2014/2015, 75% of the putative haploids were true negatives according to molecular markers. Plant vigor had a high proportion of true negatives. Molecular markers and flow cytometry are more efficient in classifying plant ploidy level. Chromosome duplication was efficient in all plants. |
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Crop Breeding and Applied Biotechnology |
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Haploid identification using tropicalized haploid inducer progenies in maizeFlow cytometryZea mayschromosome countingchromosome duplicationploidy level.Abstract The aim of this study was to identify maize haploid plants and compare the efficiency of identification of maize haploid plants using the R1-nj morphological marker, plant vigor, flow cytometry, chromosome counting, and microsatellite molecular markers under tropical conditions. We also established a protocol for chromosome duplication in maize haploid plants. Fourteen S0:1 and seven S2:3 haploid inducer progenies were crossed with GNZ9501 in 2012/2013 and 2014/2015, respectively. Through use of the R1-nj trait, we were able to identify 552 putative haploid seeds in 2012/2013 and 260 putative haploid seeds in 2014/2015. Only 1.84% were true positives according to flow cytometry in 2012/2013. In 2014/2015, 75% of the putative haploids were true negatives according to molecular markers. Plant vigor had a high proportion of true negatives. Molecular markers and flow cytometry are more efficient in classifying plant ploidy level. Chromosome duplication was efficient in all plants.Crop Breeding and Applied Biotechnology2018-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000100016Crop Breeding and Applied Biotechnology v.18 n.1 2018reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332018v18n1a3info:eu-repo/semantics/openAccessRibeiro,Camila BastosPereira,Francielly de CássiaNóbrega Filho,Lamartine daRezende,Breno AlvarengaDias,Kaio Olímpio das GraçasBraz,Guilherme TomazRuy,Murilo CandidoSilva,Maria BeatrizCenzi,GiseleTechio,Vânia HelenaSouza,João Cândido deeng2018-02-28T00:00:00Zoai:scielo:S1984-70332018000100016Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2018-02-28T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse |
dc.title.none.fl_str_mv |
Haploid identification using tropicalized haploid inducer progenies in maize |
title |
Haploid identification using tropicalized haploid inducer progenies in maize |
spellingShingle |
Haploid identification using tropicalized haploid inducer progenies in maize Ribeiro,Camila Bastos Flow cytometry Zea mays chromosome counting chromosome duplication ploidy level. |
title_short |
Haploid identification using tropicalized haploid inducer progenies in maize |
title_full |
Haploid identification using tropicalized haploid inducer progenies in maize |
title_fullStr |
Haploid identification using tropicalized haploid inducer progenies in maize |
title_full_unstemmed |
Haploid identification using tropicalized haploid inducer progenies in maize |
title_sort |
Haploid identification using tropicalized haploid inducer progenies in maize |
author |
Ribeiro,Camila Bastos |
author_facet |
Ribeiro,Camila Bastos Pereira,Francielly de Cássia Nóbrega Filho,Lamartine da Rezende,Breno Alvarenga Dias,Kaio Olímpio das Graças Braz,Guilherme Tomaz Ruy,Murilo Candido Silva,Maria Beatriz Cenzi,Gisele Techio,Vânia Helena Souza,João Cândido de |
author_role |
author |
author2 |
Pereira,Francielly de Cássia Nóbrega Filho,Lamartine da Rezende,Breno Alvarenga Dias,Kaio Olímpio das Graças Braz,Guilherme Tomaz Ruy,Murilo Candido Silva,Maria Beatriz Cenzi,Gisele Techio,Vânia Helena Souza,João Cândido de |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ribeiro,Camila Bastos Pereira,Francielly de Cássia Nóbrega Filho,Lamartine da Rezende,Breno Alvarenga Dias,Kaio Olímpio das Graças Braz,Guilherme Tomaz Ruy,Murilo Candido Silva,Maria Beatriz Cenzi,Gisele Techio,Vânia Helena Souza,João Cândido de |
dc.subject.por.fl_str_mv |
Flow cytometry Zea mays chromosome counting chromosome duplication ploidy level. |
topic |
Flow cytometry Zea mays chromosome counting chromosome duplication ploidy level. |
description |
Abstract The aim of this study was to identify maize haploid plants and compare the efficiency of identification of maize haploid plants using the R1-nj morphological marker, plant vigor, flow cytometry, chromosome counting, and microsatellite molecular markers under tropical conditions. We also established a protocol for chromosome duplication in maize haploid plants. Fourteen S0:1 and seven S2:3 haploid inducer progenies were crossed with GNZ9501 in 2012/2013 and 2014/2015, respectively. Through use of the R1-nj trait, we were able to identify 552 putative haploid seeds in 2012/2013 and 260 putative haploid seeds in 2014/2015. Only 1.84% were true positives according to flow cytometry in 2012/2013. In 2014/2015, 75% of the putative haploids were true negatives according to molecular markers. Plant vigor had a high proportion of true negatives. Molecular markers and flow cytometry are more efficient in classifying plant ploidy level. Chromosome duplication was efficient in all plants. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000100016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000100016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1984-70332018v18n1a3 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
dc.source.none.fl_str_mv |
Crop Breeding and Applied Biotechnology v.18 n.1 2018 reponame:Crop Breeding and Applied Biotechnology instname:Sociedade Brasileira de Melhoramento de Plantas instacron:CBAB |
instname_str |
Sociedade Brasileira de Melhoramento de Plantas |
instacron_str |
CBAB |
institution |
CBAB |
reponame_str |
Crop Breeding and Applied Biotechnology |
collection |
Crop Breeding and Applied Biotechnology |
repository.name.fl_str_mv |
Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas |
repository.mail.fl_str_mv |
cbabjournal@gmail.com||cbab@ufv.br |
_version_ |
1754209187536044032 |