Haploid identification using tropicalized haploid inducer progenies in maize

• Autor(a) principal: Ribeiro,Camila Bastos
• Data de Publicação: 2018
• Outros Autores: Pereira,Francielly de Cássia, Nóbrega Filho,Lamartine da, Rezende,Breno Alvarenga, Dias,Kaio Olímpio das Graças, Braz,Guilherme Tomaz, Ruy,Murilo Candido, Silva,Maria Beatriz, Cenzi,Gisele, Techio,Vânia Helena, Souza,João Cândido de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Crop Breeding and Applied Biotechnology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000100016
• topic: Flow cytometry; Zea mays; chromosome counting; chromosome duplication; ploidy level.

Abstract The aim of this study was to identify maize haploid plants and compare the efficiency of identification of maize haploid plants using the R1-nj morphological marker, plant vigor, flow cytometry, chromosome counting, and microsatellite molecular markers under tropical conditions. We also established a protocol for chromosome duplication in maize haploid plants. Fourteen S0:1 and seven S2:3 haploid inducer progenies were crossed with GNZ9501 in 2012/2013 and 2014/2015, respectively. Through use of the R1-nj trait, we were able to identify 552 putative haploid seeds in 2012/2013 and 260 putative haploid seeds in 2014/2015. Only 1.84% were true positives according to flow cytometry in 2012/2013. In 2014/2015, 75% of the putative haploids were true negatives according to molecular markers. Plant vigor had a high proportion of true negatives. Molecular markers and flow cytometry are more efficient in classifying plant ploidy level. Chromosome duplication was efficient in all plants.