Usefulness of serological ELISA assay forTaenia saginata to detect naturally infected bovines
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000200270 |
Resumo: | Bovine cysticercosis, a cosmopolitan disease caused by Taenia saginata, leads to economic losses due to carcass devaluation at slaughter. Sanitary inspection at slaughterhouses, the routine diagnostic method in Brazil, lacks the necessary sensitivity to detect the mildly infected cattle that are typically encoutered in Brazil. In this study we have tested cattle sera from animals diagnosed as positive and negative by veterianry inspection for (1) anti-parasite antibodies using metacestodes antigens (T. solium vesicular fluid and T. saginata secretions) and (2) the HP10 secreted antigen of viable metacestodes. The cut-off values were calculated by ROC curve for intense and mild infections conditions, and by the classical method ( for negative samples). The sensitivity and specificity of these diagnostic tests were different depending on the assumed cut-off value and, importantly, whether the infection was mild or intense. In spite of these observations, however, such ELISA assays for serum antibodies and parasite antigens constitute an important tool for epidemiological porposes, and in establishing priorities for the control of bovine cysticercosis. |
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Usefulness of serological ELISA assay forTaenia saginata to detect naturally infected bovinesSanitary inspectionHP10 antigen assayELISAvesicular fluidexcretion-secretionBovine cysticercosis, a cosmopolitan disease caused by Taenia saginata, leads to economic losses due to carcass devaluation at slaughter. Sanitary inspection at slaughterhouses, the routine diagnostic method in Brazil, lacks the necessary sensitivity to detect the mildly infected cattle that are typically encoutered in Brazil. In this study we have tested cattle sera from animals diagnosed as positive and negative by veterianry inspection for (1) anti-parasite antibodies using metacestodes antigens (T. solium vesicular fluid and T. saginata secretions) and (2) the HP10 secreted antigen of viable metacestodes. The cut-off values were calculated by ROC curve for intense and mild infections conditions, and by the classical method ( for negative samples). The sensitivity and specificity of these diagnostic tests were different depending on the assumed cut-off value and, importantly, whether the infection was mild or intense. In spite of these observations, however, such ELISA assays for serum antibodies and parasite antigens constitute an important tool for epidemiological porposes, and in establishing priorities for the control of bovine cysticercosis.Colégio Brasileiro de Parasitologia Veterinária2013-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000200270Revista Brasileira de Parasitologia Veterinária v.22 n.2 2013reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612013005000026info:eu-repo/semantics/openAccessPaulan,Silvana de CássiaGonzáles,Rutilia Marisela HernándesPeralta,Laura AdalidVicentini-Oliveira,Josy CampanhãBiondi,Germano FranciscoConde,Edda SciutoParkhouse,Robert Michael EvansNunes,Cáris Maronieng2015-07-30T00:00:00Zoai:scielo:S1984-29612013000200270Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2015-07-30T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Usefulness of serological ELISA assay forTaenia saginata to detect naturally infected bovines |
title |
Usefulness of serological ELISA assay forTaenia saginata to detect naturally infected bovines |
spellingShingle |
Usefulness of serological ELISA assay forTaenia saginata to detect naturally infected bovines Paulan,Silvana de Cássia Sanitary inspection HP10 antigen assay ELISA vesicular fluid excretion-secretion |
title_short |
Usefulness of serological ELISA assay forTaenia saginata to detect naturally infected bovines |
title_full |
Usefulness of serological ELISA assay forTaenia saginata to detect naturally infected bovines |
title_fullStr |
Usefulness of serological ELISA assay forTaenia saginata to detect naturally infected bovines |
title_full_unstemmed |
Usefulness of serological ELISA assay forTaenia saginata to detect naturally infected bovines |
title_sort |
Usefulness of serological ELISA assay forTaenia saginata to detect naturally infected bovines |
author |
Paulan,Silvana de Cássia |
author_facet |
Paulan,Silvana de Cássia Gonzáles,Rutilia Marisela Hernándes Peralta,Laura Adalid Vicentini-Oliveira,Josy Campanhã Biondi,Germano Francisco Conde,Edda Sciuto Parkhouse,Robert Michael Evans Nunes,Cáris Maroni |
author_role |
author |
author2 |
Gonzáles,Rutilia Marisela Hernándes Peralta,Laura Adalid Vicentini-Oliveira,Josy Campanhã Biondi,Germano Francisco Conde,Edda Sciuto Parkhouse,Robert Michael Evans Nunes,Cáris Maroni |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Paulan,Silvana de Cássia Gonzáles,Rutilia Marisela Hernándes Peralta,Laura Adalid Vicentini-Oliveira,Josy Campanhã Biondi,Germano Francisco Conde,Edda Sciuto Parkhouse,Robert Michael Evans Nunes,Cáris Maroni |
dc.subject.por.fl_str_mv |
Sanitary inspection HP10 antigen assay ELISA vesicular fluid excretion-secretion |
topic |
Sanitary inspection HP10 antigen assay ELISA vesicular fluid excretion-secretion |
description |
Bovine cysticercosis, a cosmopolitan disease caused by Taenia saginata, leads to economic losses due to carcass devaluation at slaughter. Sanitary inspection at slaughterhouses, the routine diagnostic method in Brazil, lacks the necessary sensitivity to detect the mildly infected cattle that are typically encoutered in Brazil. In this study we have tested cattle sera from animals diagnosed as positive and negative by veterianry inspection for (1) anti-parasite antibodies using metacestodes antigens (T. solium vesicular fluid and T. saginata secretions) and (2) the HP10 secreted antigen of viable metacestodes. The cut-off values were calculated by ROC curve for intense and mild infections conditions, and by the classical method ( for negative samples). The sensitivity and specificity of these diagnostic tests were different depending on the assumed cut-off value and, importantly, whether the infection was mild or intense. In spite of these observations, however, such ELISA assays for serum antibodies and parasite antigens constitute an important tool for epidemiological porposes, and in establishing priorities for the control of bovine cysticercosis. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000200270 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000200270 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1984-29612013005000026 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.22 n.2 2013 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
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1754208914914672640 |