MOLECULAR MARKERS AS AUXILIARY TOOLS IN CYTOLOGICAL DIAGNOSIS OF CERVICAL ABNORMALITIES

• Autor(a) principal: Crisóstomo, Catharine de Araújo
• Data de Publicação: 2016
• Outros Autores: Melo-Júnior, Mario Ribeiro de, Medeiros, Adrya Lúcia Peres Bezerra de
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Saúde e Pesquisa (Online)
• Texto Completo: https://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/4685
• topic: Cervical Cancer; Biological Markers; Papillomaviridae.; Cervical Cancer; Biological Markers; Papillomaviridae.; Displasia do Colo do Útero; Marcadores Biológicos; Papillomaviridae; Displasia do Colo do Útero; Marcadores Biológicos; Papillomaviridae

Cervical cancer ranks fourth as the most diagnosed neoplasm in females worldwide and third in Brazil. The cytological exam is the main approach for countering its progress. Improvement in the test´s performance comprises molecular markers, especially those involved in the regulation of the cell cycle. Detection of molecules by immunocytochemistry has a good correlation with the degree of intra-epithelial lesion and decreases the interobservor´s variability. Current research assesses scientific evidences of the efficiency of markers p16INK4A, Ki-67, TopIIα and MCM2 in patients with cervical abnormalities and/or positive to the DNA of HPV. Articles were retrieved from databases by descriptors biomarkers, molecular marker and cervical dysplasias for the systematic review of the literature, between May and June 2015. Original articles published during the last ten years (2004-2015) were included, from which 15 articles were selected. Positivity index of the four markers correlates with the seriousness of the lesion. They were capable of identifying high degree implicit intra-epithelial lesions. The immunocytochemistry stain to detect the molecules is a help in the localization of potentially abnormal cells and contributes towards the identification of females with a greater possibility in developing cervical cancer and, consequently, improvement in the sensitiveness of the cytological exam.