Integrin αDβ2 influences cerebral edema, leukocyte accumulation and neurologic outcomes in experimental severe malaria
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/40884 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. |
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Quintanilha, Isaclaudia G. de AzevedoAbreu, Adriana Vieira deFerreira, André C.Reis, Patricia AlvesSilva, Tathiany I.Nascimento, Danielle de O.Campbell, Robert A.Estato, VanessaWeyrich, Andrew S.Bozza, Patrícia T.Zimmerman, Guy A.Faria Neto, Hugo Caire C.2020-04-20T14:11:01Z2020-04-20T14:11:01Z2019QUINTANILHA, Isaclaudia G. de Azevedo et al. Integrin αDβ2 influences cerebral edema, leukocyte accumulation and neurologic outcomes in experimental severe malaria. Plos One, v. 14, n. 12, e0224610, p. 1-22, Dec. 2019.1932-6203https://www.arca.fiocruz.br/handle/icict/4088410.1371/journal.pone.0224610engPublic Library of ScienceIntegrin αDβ2Edema cerebralAcumulação de leucócitosResultados neurológicosMalária grave experimentalIntegrin αDβ2Cerebral edemaLeukocyte accumulationNeurologic outcomesExperimental severe malariaIntegrin αDβ2 influences cerebral edema, leukocyte accumulation and neurologic outcomes in experimental severe malariainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.University of Utah. Department of Internal Medicine and Program in Molecular Medicine. Salt Lake City, Utah, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.University of Utah. Department of Internal Medicine and Program in Molecular Medicine. Salt Lake City, Utah, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.University of Utah. Department of Internal Medicine and Program in Molecular Medicine. Salt Lake City, Utah, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Malaria is an infectious disease of major worldwide clinical importance that causes a variety of severe, or complicated, syndromes including cerebral malaria, which is often fatal. Leukocyte integrins are essential for host defense but also mediate physiologic responses of the innate and adaptive immune systems. We previously showed that targeted deletion of the αD subunit (αD-/-) of the αDβ2 integrin, which is expressed on key leukocyte subsets in mice and humans, leads to absent expression of the integrin heterodimer on murine macrophages and reduces mortality in mice infected with Plasmodium berghei ANKA (P. berghei ANKA). To further identify mechanisms involved in the protective effect of αD deletion in this model of severe malaria we examined wild type C57BL/6 (WT) and αD-/- mice after P. berghei ANKA infection and found that vessel plugging and leukocyte infiltration were significantly decreased in the brains of αD-/- animals. Intravital microscopy demonstrated decreased rolling and adhesion of leukocytes in cerebral vessels of αD-/- mice. Flow cytometry analysis showed decreased T-lymphocyte accumulation in the brains of infected αD-/- animals. Evans blue dye exclusion assays demonstrated significantly less dye extravasation in the brains of αD-/- mice, indicating preserved blood-brain barrier integrity. WT mice that were salvaged from P. berghei ANKA infection by treatment with chloroquine had impaired aversive memory, which was not observed in αD-/- mice. We conclude that deletion of integrin αDβ2 alters the natural course of experimental severe malaria, demonstrating previously unrecognized activities of a key leukocyte integrin in immune-inflammatory responses that mediate cerebral involvement.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/40884/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAdrianaAbreu_HugoCFariaNeto_etal_2019.pdfAdrianaAbreu_HugoCFariaNeto_etal_2019.pdfapplication/pdf2808904https://www.arca.fiocruz.br/bitstream/icict/40884/2/AdrianaAbreu_HugoCFariaNeto_etal_2019.pdfd03aa49cbc6bfb83e5cbdf37726052a6MD52TEXTAdrianaAbreu_HugoCFariaNeto_etal_2019.pdf.txtAdrianaAbreu_HugoCFariaNeto_etal_2019.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Integrin αDβ2 influences cerebral edema, leukocyte accumulation and neurologic outcomes in experimental severe malaria |
| title |
Integrin αDβ2 influences cerebral edema, leukocyte accumulation and neurologic outcomes in experimental severe malaria |
| spellingShingle |
Integrin αDβ2 influences cerebral edema, leukocyte accumulation and neurologic outcomes in experimental severe malaria Quintanilha, Isaclaudia G. de Azevedo Integrin αDβ2 Edema cerebral Acumulação de leucócitos Resultados neurológicos Malária grave experimental Integrin αDβ2 Cerebral edema Leukocyte accumulation Neurologic outcomes Experimental severe malaria |
| title_short |
Integrin αDβ2 influences cerebral edema, leukocyte accumulation and neurologic outcomes in experimental severe malaria |
| title_full |
Integrin αDβ2 influences cerebral edema, leukocyte accumulation and neurologic outcomes in experimental severe malaria |
| title_fullStr |
Integrin αDβ2 influences cerebral edema, leukocyte accumulation and neurologic outcomes in experimental severe malaria |
| title_full_unstemmed |
Integrin αDβ2 influences cerebral edema, leukocyte accumulation and neurologic outcomes in experimental severe malaria |
| title_sort |
Integrin αDβ2 influences cerebral edema, leukocyte accumulation and neurologic outcomes in experimental severe malaria |
| author |
Quintanilha, Isaclaudia G. de Azevedo |
| author_facet |
Quintanilha, Isaclaudia G. de Azevedo Abreu, Adriana Vieira de Ferreira, André C. Reis, Patricia Alves Silva, Tathiany I. Nascimento, Danielle de O. Campbell, Robert A. Estato, Vanessa Weyrich, Andrew S. Bozza, Patrícia T. Zimmerman, Guy A. Faria Neto, Hugo Caire C. |
| author_role |
author |
| author2 |
Abreu, Adriana Vieira de Ferreira, André C. Reis, Patricia Alves Silva, Tathiany I. Nascimento, Danielle de O. Campbell, Robert A. Estato, Vanessa Weyrich, Andrew S. Bozza, Patrícia T. Zimmerman, Guy A. Faria Neto, Hugo Caire C. |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Quintanilha, Isaclaudia G. de Azevedo Abreu, Adriana Vieira de Ferreira, André C. Reis, Patricia Alves Silva, Tathiany I. Nascimento, Danielle de O. Campbell, Robert A. Estato, Vanessa Weyrich, Andrew S. Bozza, Patrícia T. Zimmerman, Guy A. Faria Neto, Hugo Caire C. |
| dc.subject.other.pt_BR.fl_str_mv |
Integrin αDβ2 Edema cerebral Acumulação de leucócitos Resultados neurológicos Malária grave experimental |
| topic |
Integrin αDβ2 Edema cerebral Acumulação de leucócitos Resultados neurológicos Malária grave experimental Integrin αDβ2 Cerebral edema Leukocyte accumulation Neurologic outcomes Experimental severe malaria |
| dc.subject.en.pt_BR.fl_str_mv |
Integrin αDβ2 Cerebral edema Leukocyte accumulation Neurologic outcomes Experimental severe malaria |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. |
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2019 |
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2019 |
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2020-04-20T14:11:01Z |
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2020-04-20T14:11:01Z |
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info:eu-repo/semantics/publishedVersion |
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QUINTANILHA, Isaclaudia G. de Azevedo et al. Integrin αDβ2 influences cerebral edema, leukocyte accumulation and neurologic outcomes in experimental severe malaria. Plos One, v. 14, n. 12, e0224610, p. 1-22, Dec. 2019. |
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https://www.arca.fiocruz.br/handle/icict/40884 |
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1932-6203 |
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10.1371/journal.pone.0224610 |
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QUINTANILHA, Isaclaudia G. de Azevedo et al. Integrin αDβ2 influences cerebral edema, leukocyte accumulation and neurologic outcomes in experimental severe malaria. Plos One, v. 14, n. 12, e0224610, p. 1-22, Dec. 2019. 1932-6203 10.1371/journal.pone.0224610 |
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