Yeast-derived human immunodeficiency virus type 1 p55(gag) virus-like particles activate dendritic cells (DCs) and induce perforin expression in Gag-specific CD8(+) T cells by cross-presentation of DCs
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/15242 |
Resumo: | National Institute of Infectious Diseases. Department of Immunology. Shinjuku-ku |
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Tsunetsugu-Yokota, YasukoMorikawa, YukoIsogai, MayaKawana-Tachikawa, AiOdawara, TakashiNakamura, TetsuyaGrassi, Maria Fernanda RiosAutran, BrigitteIwamoto, Aikichi2016-08-11T16:15:38Z2016-08-11T16:15:38Z2003TSUNETSUGU-YOKOTA, Y. et al. Yeast-derived human immunodeficiency virus type 1 p55(gag) virus-like particles activate dendritic cells (DCs) and induce perforin expression in Gag-specific CD8(+) T cells by cross-presentation of DCs. Journal of Virology, v. 77, n. 19, p. 10250–10259, 2003.0022-538Xhttps://www.arca.fiocruz.br/handle/icict/1524210.1128/JVI.77.19.10250–10259.2003engAmerican Society for MicrobiologyYeast-derived human immunodeficiency virus type 1 p55(gag) virus-like particles activate dendritic cells (DCs) and induce perforin expression in Gag-specific CD8(+) T cells by cross-presentation of DCsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNational Institute of Infectious Diseases. Department of Immunology. Shinjuku-kuKitasato University. Kitasato Institute for Life SciencesNational Institute of Infectious Diseases. Department of Immunology. Shinjuku-kuUniversity of Tokyo. Institute of Medical Science. Department of Infectious Diseases. TokyoUniversity of Tokyo. Institute of Medical Science. Department of Infectious Diseases. TokyoUniversity of Tokyo. Institute of Medical Science. Department of Infectious Diseases. TokyoFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, BrasilHôpital Pitié-Salpêtrière. Laboratoire d’Immunologie Cellulaire et Tissulaire. Paris, FranceUniversity of Tokyo. Institute of Medical Science. Department of Infectious Diseases. TokyoTo evaluate the immunogenicity of human immunodeficiency virus (HIV) type 1 p55(gag) virus-like particles (VLPs) released by budding from yeast spheroplasts, we have analyzed the effects of yeast VLPs on monocyte-derived dendritic cells (DCs). Yeast VLPs were efficiently incorporated into DCs via both macropinocytosis and endocytosis mediated by mannose-recognizing receptors, but not the mannose receptor. The uptake of yeast VLPs induced DC maturation and enhanced cytokine production, notably, interleukin-12 p70. We showed that yeast membrane components may contribute to DC maturation partly through Toll-like receptor 2 signaling. Thus, Gag particles encapsulated by yeast membrane may have an advantage in stimulating Gag-specific immune responses. We found that yeast VLPs, but not the control yeast membrane fraction, were able to activate both CD4(+) and CD8(+) T cells of HIV-infected individuals. We tested the effect of cross-presentation of VLP by DCs in two subjects recruited into a long-term nonprogressor-slow progressor cohort. When yeast VLP-loaded DCs of these patients were cocultured with peripheral blood mononuclear cells for 7 days, approximately one-third of the Gag-specific CD8(+) T cells were activated and became perforin positive. However, some of the Gag-specific CD8(+) T cells appeared to be lost during in vitro culture, especially in a patient with a high virus load. Our results suggest that DCs loaded with yeast VLPs can activate Gag-specific memory CD8(+) T cells to become effector cells in chronically HIV-infected individuals, but there still remain unresponsive Gag-specific T-cell populations in these patients.HIV-1Dendritic CellsInterleukin-12CD8-Positive T-LymphocytesInterferon-gammaMembrane GlycoproteinsHumansProtein Precursorsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15242/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALTsunetsugu-yokota Y Yeast-derived....pdfTsunetsugu-yokota Y Yeast-derived....pdfapplication/pdf1239987https://www.arca.fiocruz.br/bitstream/icict/15242/2/Tsunetsugu-yokota%20Y%20Yeast-derived....pdfe32fe6a5c73a8f7fdbb62398dcb7d056MD52TEXTTsunetsugu-yokota Y Yeast-derived....pdf.txtTsunetsugu-yokota Y 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dc.title.pt_BR.fl_str_mv |
Yeast-derived human immunodeficiency virus type 1 p55(gag) virus-like particles activate dendritic cells (DCs) and induce perforin expression in Gag-specific CD8(+) T cells by cross-presentation of DCs |
title |
Yeast-derived human immunodeficiency virus type 1 p55(gag) virus-like particles activate dendritic cells (DCs) and induce perforin expression in Gag-specific CD8(+) T cells by cross-presentation of DCs |
spellingShingle |
Yeast-derived human immunodeficiency virus type 1 p55(gag) virus-like particles activate dendritic cells (DCs) and induce perforin expression in Gag-specific CD8(+) T cells by cross-presentation of DCs Tsunetsugu-Yokota, Yasuko HIV-1 Dendritic Cells Interleukin-12 CD8-Positive T-Lymphocytes Interferon-gamma Membrane Glycoproteins Humans Protein Precursors |
title_short |
Yeast-derived human immunodeficiency virus type 1 p55(gag) virus-like particles activate dendritic cells (DCs) and induce perforin expression in Gag-specific CD8(+) T cells by cross-presentation of DCs |
title_full |
Yeast-derived human immunodeficiency virus type 1 p55(gag) virus-like particles activate dendritic cells (DCs) and induce perforin expression in Gag-specific CD8(+) T cells by cross-presentation of DCs |
title_fullStr |
Yeast-derived human immunodeficiency virus type 1 p55(gag) virus-like particles activate dendritic cells (DCs) and induce perforin expression in Gag-specific CD8(+) T cells by cross-presentation of DCs |
title_full_unstemmed |
Yeast-derived human immunodeficiency virus type 1 p55(gag) virus-like particles activate dendritic cells (DCs) and induce perforin expression in Gag-specific CD8(+) T cells by cross-presentation of DCs |
title_sort |
Yeast-derived human immunodeficiency virus type 1 p55(gag) virus-like particles activate dendritic cells (DCs) and induce perforin expression in Gag-specific CD8(+) T cells by cross-presentation of DCs |
author |
Tsunetsugu-Yokota, Yasuko |
author_facet |
Tsunetsugu-Yokota, Yasuko Morikawa, Yuko Isogai, Maya Kawana-Tachikawa, Ai Odawara, Takashi Nakamura, Tetsuya Grassi, Maria Fernanda Rios Autran, Brigitte Iwamoto, Aikichi |
author_role |
author |
author2 |
Morikawa, Yuko Isogai, Maya Kawana-Tachikawa, Ai Odawara, Takashi Nakamura, Tetsuya Grassi, Maria Fernanda Rios Autran, Brigitte Iwamoto, Aikichi |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Tsunetsugu-Yokota, Yasuko Morikawa, Yuko Isogai, Maya Kawana-Tachikawa, Ai Odawara, Takashi Nakamura, Tetsuya Grassi, Maria Fernanda Rios Autran, Brigitte Iwamoto, Aikichi |
dc.subject.en.pt_BR.fl_str_mv |
HIV-1 Dendritic Cells Interleukin-12 CD8-Positive T-Lymphocytes Interferon-gamma Membrane Glycoproteins Humans Protein Precursors |
topic |
HIV-1 Dendritic Cells Interleukin-12 CD8-Positive T-Lymphocytes Interferon-gamma Membrane Glycoproteins Humans Protein Precursors |
description |
National Institute of Infectious Diseases. Department of Immunology. Shinjuku-ku |
publishDate |
2003 |
dc.date.issued.fl_str_mv |
2003 |
dc.date.accessioned.fl_str_mv |
2016-08-11T16:15:38Z |
dc.date.available.fl_str_mv |
2016-08-11T16:15:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
TSUNETSUGU-YOKOTA, Y. et al. Yeast-derived human immunodeficiency virus type 1 p55(gag) virus-like particles activate dendritic cells (DCs) and induce perforin expression in Gag-specific CD8(+) T cells by cross-presentation of DCs. Journal of Virology, v. 77, n. 19, p. 10250–10259, 2003. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/15242 |
dc.identifier.issn.pt_BR.fl_str_mv |
0022-538X |
dc.identifier.eissn.none.fl_str_mv |
10.1128/JVI.77.19.10250–10259.2003 |
identifier_str_mv |
TSUNETSUGU-YOKOTA, Y. et al. Yeast-derived human immunodeficiency virus type 1 p55(gag) virus-like particles activate dendritic cells (DCs) and induce perforin expression in Gag-specific CD8(+) T cells by cross-presentation of DCs. Journal of Virology, v. 77, n. 19, p. 10250–10259, 2003. 0022-538X 10.1128/JVI.77.19.10250–10259.2003 |
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https://www.arca.fiocruz.br/handle/icict/15242 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
American Society for Microbiology |
publisher.none.fl_str_mv |
American Society for Microbiology |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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