Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates

Detalhes bibliográficos
Autor(a) principal: Barbosa, Alynne da Silva
Data de Publicação: 2018
Outros Autores: Barbosa, Helene Santos, Souza, Sandra Maria de Oliveira, Dib, Laís Verdan, Uchôa, Claudia Maria Antunes, Bastos, Otilio Machado Pereira, Amendoeira, Maria Regina Reis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31783
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozooses. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia.. Niterói, RJ, Brasil.
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spelling Barbosa, Alynne da SilvaBarbosa, Helene SantosSouza, Sandra Maria de OliveiraDib, Laís VerdanUchôa, Claudia Maria AntunesBastos, Otilio Machado PereiraAmendoeira, Maria Regina Reis2019-02-20T13:47:22Z2019-02-20T13:47:22Z2018BARBOSA, Alynne da Silva et al. Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates. Acta Parasitologica, v. 63, n. 3, p. 287-298, Jun. 2018.1230-2821https://www.arca.fiocruz.br/handle/icict/3178310.1515/ap-2018-00331896-1851engDe Gruyter OpenBalantioides coliCiliadaPeroxissomosBalantioides coliCiliateSEMTEMHydrogenosomes likePeroxisomesBalantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolatesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozooses. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia.. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia.. Niterói, RJ, Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia.. Niterói, RJ, Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia.. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozooses. Rio de Janeiro, RJ, Brasil.Balantioides coli is a ciliated protozoon that inhabits the intestine of pigs, non-human primates and humans. Light microscopy studies have described over 50 species of the genus Balantioides but their validity is in doubt. Due to the limited information about this genus, this study is aimed to identify morphological characteristics of Balantioides coli isolated using fluorescence microscopy and both scanning (SEM) and transmission electron microscopy (TEM). Trophozoites isolated from the feces of pig and macaque were washed and subjected to centrifugation. These cells were fixed with paraformaldehyde for immunofluorescence. Other aliquots of these trophozoites were fixed with glutaraldehyde, post fixed with osmium tetroxide and processed for SEM and TEM. Immunofluorescence studies revealed microtubules with a longitudinal distribution to the main axis of the parasite and in the constitution of cilia. SEM demonstrated a high concentration of cilia covering the oral apparatus and a poor presence of such structures in cytopyge. TEM revealed in the plasma membrane, several associated structures were observed to delineate the cellular cortex and mucocysts. The cytoskeleton of the oral region was observed in detail and had an organization pattern consisting of microtubules, which formed files and nematodesmal networks. Organelles such as hydrogenosomes like and peroxisomes were observed close to the cortex. Macronuclei were observed, but structures that were consistent with micronuclei were not identified. Ultrastructural morphological analysis of isolates confirms its similarity to Balantioides coli. In this study were identified structures that had not yet been described, such as hydrogenosomes like and cytoskeletal structures.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/31783/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALalinesbarbosa_etal_IOC_2018.pdfalinesbarbosa_etal_IOC_2018.pdfapplication/pdf80110https://www.arca.fiocruz.br/bitstream/icict/31783/2/alinesbarbosa_etal_IOC_2018.pdf02ebf39cf676681da6536dd6c894fd47MD52TEXTalinesbarbosa_etal_IOC_2018.pdf.txtalinesbarbosa_etal_IOC_2018.pdf.txtExtracted texttext/plain10332https://www.arca.fiocruz.br/bitstream/icict/31783/3/alinesbarbosa_etal_IOC_2018.pdf.txt13f62f3e06a6dc085a8fd75b3f033519MD53icict/317832019-02-22 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dc.title.pt_BR.fl_str_mv Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates
title Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates
spellingShingle Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates
Barbosa, Alynne da Silva
Balantioides coli
Ciliada
Peroxissomos
Balantioides coli
Ciliate
SEM
TEM
Hydrogenosomes like
Peroxisomes
title_short Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates
title_full Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates
title_fullStr Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates
title_full_unstemmed Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates
title_sort Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates
author Barbosa, Alynne da Silva
author_facet Barbosa, Alynne da Silva
Barbosa, Helene Santos
Souza, Sandra Maria de Oliveira
Dib, Laís Verdan
Uchôa, Claudia Maria Antunes
Bastos, Otilio Machado Pereira
Amendoeira, Maria Regina Reis
author_role author
author2 Barbosa, Helene Santos
Souza, Sandra Maria de Oliveira
Dib, Laís Verdan
Uchôa, Claudia Maria Antunes
Bastos, Otilio Machado Pereira
Amendoeira, Maria Regina Reis
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barbosa, Alynne da Silva
Barbosa, Helene Santos
Souza, Sandra Maria de Oliveira
Dib, Laís Verdan
Uchôa, Claudia Maria Antunes
Bastos, Otilio Machado Pereira
Amendoeira, Maria Regina Reis
dc.subject.other.pt_BR.fl_str_mv Balantioides coli
Ciliada
Peroxissomos
topic Balantioides coli
Ciliada
Peroxissomos
Balantioides coli
Ciliate
SEM
TEM
Hydrogenosomes like
Peroxisomes
dc.subject.en.pt_BR.fl_str_mv Balantioides coli
Ciliate
SEM
TEM
Hydrogenosomes like
Peroxisomes
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozooses. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia.. Niterói, RJ, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-02-20T13:47:22Z
dc.date.available.fl_str_mv 2019-02-20T13:47:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARBOSA, Alynne da Silva et al. Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates. Acta Parasitologica, v. 63, n. 3, p. 287-298, Jun. 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31783
dc.identifier.issn.pt_BR.fl_str_mv 1230-2821
dc.identifier.doi.none.fl_str_mv 10.1515/ap-2018-0033
dc.identifier.eissn.none.fl_str_mv 1896-1851
identifier_str_mv BARBOSA, Alynne da Silva et al. Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates. Acta Parasitologica, v. 63, n. 3, p. 287-298, Jun. 2018.
1230-2821
10.1515/ap-2018-0033
1896-1851
url https://www.arca.fiocruz.br/handle/icict/31783
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv De Gruyter Open
publisher.none.fl_str_mv De Gruyter Open
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/31783/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/31783/2/alinesbarbosa_etal_IOC_2018.pdf
https://www.arca.fiocruz.br/bitstream/icict/31783/3/alinesbarbosa_etal_IOC_2018.pdf.txt
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bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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