Eudesmol isomers induce caspase-mediated apoptosis in human hepatocellular carcinoma HepG2 cells

Detalhes bibliográficos
Autor(a) principal: Bomfim, Diogo S.
Data de Publicação: 2013
Outros Autores: Ferraz, Rosana Paula Cruz, Carvalho, Nanashara Coelho de, Soares, Milena Botelho Pereira, Pinheiro, Maria Lúcia Bélem, Costa, Emmanoel Vilaça, Bezerra, Daniel Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8565
Resumo: Federal University of Sergipe. Department of Physiology. São Crist ovão. Aracaju, SE, Brasil.
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spelling Bomfim, Diogo S.Ferraz, Rosana Paula CruzCarvalho, Nanashara Coelho deSoares, Milena Botelho PereiraPinheiro, Maria Lúcia BélemCosta, Emmanoel VilaçaBezerra, Daniel Pereira2014-10-09T14:02:22Z2014-10-09T14:02:22Z2013BOMFIM, Diogo D. et al. Eudesmol isomers induce caspase-mediated apoptosis in human hepatocellular carcinoma HepG2 cells. Basic & Clinical Pharmacology & Toxicology, v. 113, n. 5, p. 300-306, 2013.1742-7843https://www.arca.fiocruz.br/handle/icict/856510.1111/bcpt.12097engJohn Wiley & Sons LtdEudesmol isomers induce caspase-mediated apoptosis in human hepatocellular carcinoma HepG2 cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Sergipe. Department of Physiology. São Crist ovão. Aracaju, SE, Brasil.Federal University of Sergipe. Department of Physiology. São Crist ovão. Aracaju, SE, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brasil.Federal University of Sergipe. Department of Chemistry. São Cristovão. Aracaju, SE, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Eudesmols are naturally occurring sesquiterpenoid alcohols that present cytotoxic effect to cancer cells. Herein, all eudesmol isomers displayed cytotoxicity to different tumour cell lines. a-Eudesmol showed IC50 values ranging from 5.38 1.10 to 10.60 1.33 lg/mL for B16-F10 and K562 cell lines, b-eudesmol showed IC50 values ranging from 16.51 1.21 to 24.57 2.75 lg/mL for B16-F10 and HepG2 cell lines, and c-eudesmol showed IC50 values ranging from 8.86 1.27 to 15.15 1.06 lg/mL for B16-F10 and K562 cell lines, respectively. In addition, in this work, we studied the mechanisms of cytotoxic action of eudesmol isomers (a-, b- and c-eudesmol) in human hepatocellular carcinoma HepG2 cells. After 24-hr incubation, HepG2 cells treated with eudesmol isomers presented typical hallmarks of apoptosis, as observed by morphological analysis in cells stained with haematoxylin–eosin and acridine orange/ethidium bromide. None of eudesmol isomers caused membrane disruption at any concentration tested. Moreover, eudesmol isomers induced loss of mitochondrial membrane potential and an increase in caspase-3 activation in HepG2 cells, suggesting the induction of caspase-mediated apoptotic cell death. In conclusion, the eudesmol isomers herein investigated are able to reduce cell proliferation and to induce tumour cell death by caspase-mediated apoptosis pathways.Eudesmol IsomersHuman HepatocellularCarcinoma HepG2 CellsApoptose/efeitos de drogasSesquiterpenos de Eudesmano/farmacologiaCarcinoma Hepatocelular/metabolismoCarcinoma Hepatocelular/patologiaCaspase 3/genéticaCaspase 3/metabolismoLinhagem Celular TumoralMembrana Celular/efeitos de drogasProliferação de Células/efeitos de drogasCélulas Hep G2HumanosConcentração Inibidora 50Células K562Potencial da Membrana Mitocondrial/efeitos de drogasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8565/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALBomfim D S Eudesmol isomers....pdfBomfim D S Eudesmol isomers....pdfapplication/pdf347481https://www.arca.fiocruz.br/bitstream/icict/8565/2/Bomfim%20D%20S%20Eudesmol%20isomers....pdfc135fbf342d3987d185b0543c1fd8afeMD52TEXTBomfim D S Eudesmol isomers....pdf.txtBomfim D S Eudesmol isomers....pdf.txtExtracted texttext/plain34748https://www.arca.fiocruz.br/bitstream/icict/8565/3/Bomfim%20D%20S%20Eudesmol%20isomers....pdf.txt06f9e4be37d2a8d8b020b7a82dcc3437MD53icict/85652023-03-15 14:33:21.712oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Eudesmol isomers induce caspase-mediated apoptosis in human hepatocellular carcinoma HepG2 cells
title Eudesmol isomers induce caspase-mediated apoptosis in human hepatocellular carcinoma HepG2 cells
spellingShingle Eudesmol isomers induce caspase-mediated apoptosis in human hepatocellular carcinoma HepG2 cells
Bomfim, Diogo S.
Eudesmol Isomers
Human Hepatocellular
Carcinoma HepG2 Cells
Apoptose/efeitos de drogas
Sesquiterpenos de Eudesmano/farmacologia
Carcinoma Hepatocelular/metabolismo
Carcinoma Hepatocelular/patologia
Caspase 3/genética
Caspase 3/metabolismo
Linhagem Celular Tumoral
Membrana Celular/efeitos de drogas
Proliferação de Células/efeitos de drogas
Células Hep G2
Humanos
Concentração Inibidora 50
Células K562
Potencial da Membrana Mitocondrial/efeitos de drogas
title_short Eudesmol isomers induce caspase-mediated apoptosis in human hepatocellular carcinoma HepG2 cells
title_full Eudesmol isomers induce caspase-mediated apoptosis in human hepatocellular carcinoma HepG2 cells
title_fullStr Eudesmol isomers induce caspase-mediated apoptosis in human hepatocellular carcinoma HepG2 cells
title_full_unstemmed Eudesmol isomers induce caspase-mediated apoptosis in human hepatocellular carcinoma HepG2 cells
title_sort Eudesmol isomers induce caspase-mediated apoptosis in human hepatocellular carcinoma HepG2 cells
author Bomfim, Diogo S.
author_facet Bomfim, Diogo S.
Ferraz, Rosana Paula Cruz
Carvalho, Nanashara Coelho de
Soares, Milena Botelho Pereira
Pinheiro, Maria Lúcia Bélem
Costa, Emmanoel Vilaça
Bezerra, Daniel Pereira
author_role author
author2 Ferraz, Rosana Paula Cruz
Carvalho, Nanashara Coelho de
Soares, Milena Botelho Pereira
Pinheiro, Maria Lúcia Bélem
Costa, Emmanoel Vilaça
Bezerra, Daniel Pereira
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bomfim, Diogo S.
Ferraz, Rosana Paula Cruz
Carvalho, Nanashara Coelho de
Soares, Milena Botelho Pereira
Pinheiro, Maria Lúcia Bélem
Costa, Emmanoel Vilaça
Bezerra, Daniel Pereira
dc.subject.en.pt_BR.fl_str_mv Eudesmol Isomers
Human Hepatocellular
Carcinoma HepG2 Cells
topic Eudesmol Isomers
Human Hepatocellular
Carcinoma HepG2 Cells
Apoptose/efeitos de drogas
Sesquiterpenos de Eudesmano/farmacologia
Carcinoma Hepatocelular/metabolismo
Carcinoma Hepatocelular/patologia
Caspase 3/genética
Caspase 3/metabolismo
Linhagem Celular Tumoral
Membrana Celular/efeitos de drogas
Proliferação de Células/efeitos de drogas
Células Hep G2
Humanos
Concentração Inibidora 50
Células K562
Potencial da Membrana Mitocondrial/efeitos de drogas
dc.subject.decs.pt_BR.fl_str_mv Apoptose/efeitos de drogas
Sesquiterpenos de Eudesmano/farmacologia
Carcinoma Hepatocelular/metabolismo
Carcinoma Hepatocelular/patologia
Caspase 3/genética
Caspase 3/metabolismo
Linhagem Celular Tumoral
Membrana Celular/efeitos de drogas
Proliferação de Células/efeitos de drogas
Células Hep G2
Humanos
Concentração Inibidora 50
Células K562
Potencial da Membrana Mitocondrial/efeitos de drogas
description Federal University of Sergipe. Department of Physiology. São Crist ovão. Aracaju, SE, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-10-09T14:02:22Z
dc.date.available.fl_str_mv 2014-10-09T14:02:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BOMFIM, Diogo D. et al. Eudesmol isomers induce caspase-mediated apoptosis in human hepatocellular carcinoma HepG2 cells. Basic & Clinical Pharmacology & Toxicology, v. 113, n. 5, p. 300-306, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8565
dc.identifier.issn.none.fl_str_mv 1742-7843
dc.identifier.doi.none.fl_str_mv 10.1111/bcpt.12097
identifier_str_mv BOMFIM, Diogo D. et al. Eudesmol isomers induce caspase-mediated apoptosis in human hepatocellular carcinoma HepG2 cells. Basic & Clinical Pharmacology & Toxicology, v. 113, n. 5, p. 300-306, 2013.
1742-7843
10.1111/bcpt.12097
url https://www.arca.fiocruz.br/handle/icict/8565
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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