Hepatic damage associated with dengue-2 virus replication in liver cells of BALB/c mice

Detalhes bibliográficos
Autor(a) principal: Paes, Marciano Viana
Data de Publicação: 2009
Outros Autores: Lenzi, Henrique Leonel, Nogueira, Ana Cristina Martins, Nuovo, Gerard James, Pinhão, Ângela Teixeira, Mota, Ester Maria, Oliveira, Carlos Alberto Basilio de, Schatzmayr, Hermann, Barth, Ortrud Monika, Alves, Ada Maria de Barcelos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/3737
Resumo: We thank Dr Rita Nogueira (Laboratory of Flaviviruses, Instituto Oswaldo Cruz, Fiocruz) for supplying the dengue 2 isolate and Dr Ricardo Galler (BioManguinhos, Fiocruz) for the anti-DENV-2 serum raised in monkey. We are also grateful for the technical assistance of Aline Vilas Boas Vianna.
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spelling Paes, Marciano VianaLenzi, Henrique LeonelNogueira, Ana Cristina MartinsNuovo, Gerard JamesPinhão, Ângela TeixeiraMota, Ester MariaOliveira, Carlos Alberto Basilio deSchatzmayr, HermannBarth, Ortrud MonikaAlves, Ada Maria de Barcelos2012-01-17T17:45:09Z2012-01-17T17:45:09Z2009PAES, M. V. et al. Hepatic damage associated with dengue-2 virus replication in liver cells of BALB/c mice. Laboratory Investigation. v. 89, n. 10, p. 1140-51, Oct. 2009.0023-6837PMID:19721415https://www.arca.fiocruz.br/handle/icict/3737We thank Dr Rita Nogueira (Laboratory of Flaviviruses, Instituto Oswaldo Cruz, Fiocruz) for supplying the dengue 2 isolate and Dr Ricardo Galler (BioManguinhos, Fiocruz) for the anti-DENV-2 serum raised in monkey. We are also grateful for the technical assistance of Aline Vilas Boas Vianna.This work was supported by PDTIS-Fiocruz, CNPq, FAPERJ and Instituto do Mileˆnio-MCT.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade. Laboratório de Vacinas Virais. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Ohio State University Medical Center. Department of Pathology. Columbus, OH, Estados Unidos.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Estado do Rio de Janeiro. Hospital Universitário Gaffrée Guinle. Departamento de Anatomia Patológica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ, Brasil.One difficulty in studying dengue virus (DENV) is the lack of an experimental model that reproduces the human disease. In a previous work, we have shown that BALB/c mice intraperitoneally inoculated with a DENV-2 isolate presented viremia and mild focal areas of liver injuries. In this study, mice were inoculated by the intravenous route and presented extensive damage areas in the liver tissue, which were evaluated by histopathological and ultrastructural analysis. Hepatic injury was noted mainly around the central vein and portal tracts. Damages consist of hepatocyte injury, including steatosis, swelling and necrosis. Further, erythrophagocytosis, intercellular edema and vascular damages were evident, including hemorrhage, which is characteristic of the dengue-induced hepatitis in human liver. Hepatic lesions were already noted 2 days post infection (p.i.), although effects were more extensive after the seventh day p.i. An increase in alanine aminotransferase and aspartate aminotransferase serum levels was detected 7 and 14 days p.i., respectively, and had correlation to hepatic lesions. Alterations caused by the DENV infection were self-limiting, with a remarkable reduction of all liver damages 49 days p.i. Virus antigens were detected in hepatocytes, Kupffer cells and vascular endothelium, suggesting virus replication in these cells. In situ hybridization, using a probe that anneals in the virus negative RNA strand, showed positive reaction in hepatocytes and vascular endothelium cells of infected mice, thus confirming virus replication in such cells. In general, results revealed that this mouse model reproduces some histopathological effects observed in humans and supports previous findings indicating virus replication in the hepatic tissue.engNature Publishing GroupHepatic damage associated with dengue-2 virus replication in liver cells of BALB/c miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDengue VirusHistopathologyIn Situ HybridizationLiver DamageMouse ModelTransaminaseVírus da DengueFígadoPatologiaTransaminasesHibridização In Situinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALHepatic damage associated with dengue-2 virus replication i.pdfHepatic damage associated with dengue-2 virus replication i.pdfapplication/pdf1393233https://www.arca.fiocruz.br/bitstream/icict/3737/1/Hepatic%20damage%20associated%20with%20dengue-2%20virus%20replication%20i.pdf323554f96993267a172ad968f54c11f2MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/3737/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTHepatic damage associated with dengue-2 virus replication i.pdf.txtHepatic damage associated with dengue-2 virus replication i.pdf.txtExtracted texttext/plain44588https://www.arca.fiocruz.br/bitstream/icict/3737/5/Hepatic%20damage%20associated%20with%20dengue-2%20virus%20replication%20i.pdf.txtca8c7b5a8f68c148ebf123dbdb25627bMD55THUMBNAILHepatic damage associated with dengue-2 virus replication i.pdf.jpgHepatic damage associated with dengue-2 virus replication i.pdf.jpgGenerated Thumbnailimage/jpeg2602https://www.arca.fiocruz.br/bitstream/icict/3737/4/Hepatic%20damage%20associated%20with%20dengue-2%20virus%20replication%20i.pdf.jpg3a38467691abafe808a235c1131f8316MD54icict/37372018-04-06 09:06:40.192oai:www.arca.fiocruz.br:icict/3737Tk9OLUVYQ0xVU0lWRSBESVNUUklCVVRJT04gTElDRU5TRQoKQnkgc2lnbmluZyBhbmQgc3VibWl0dGluZyB0aGlzIGxpY2Vuc2UsIHlvdSAodGhlIGF1dGhvcihzKSBvciBjb3B5cmlnaHQKb3duZXIpIGdyYW50cyB0byBEU3BhY2UgVW5pdmVyc2l0eSAoRFNVKSB0aGUgbm9uLWV4Y2x1c2l2ZSByaWdodCB0byByZXByb2R1Y2UsCnRyYW5zbGF0ZSAoYXMgZGVmaW5lZCBiZWxvdyksIGFuZC9vciBkaXN0cmlidXRlIHlvdXIgc3VibWlzc2lvbiAoaW5jbHVkaW5nCnRoZSBhYnN0cmFjdCkgd29ybGR3aWRlIGluIHByaW50IGFuZCBlbGVjdHJvbmljIGZvcm1hdCBhbmQgaW4gYW55IG1lZGl1bSwKaW5jbHVkaW5nIGJ1dCBub3QgbGltaXRlZCB0byBhdWRpbyBvciB2aWRlby4KCllvdSBhZ3JlZSB0aGF0IERTVSBtYXksIHdpdGhvdXQgY2hhbmdpbmcgdGhlIGNvbnRlbnQsIHRyYW5zbGF0ZSB0aGUKc3VibWlzc2lvbiB0byBhbnkgbWVkaXVtIG9yIGZvcm1hdCBmb3IgdGhlIHB1cnBvc2Ugb2YgcHJlc2VydmF0aW9uLgoKWW91IGFsc28gYWdyZWUgdGhhdCBEU1UgbWF5IGtlZXAgbW9yZSB0aGFuIG9uZSBjb3B5IG9mIHRoaXMgc3VibWlzc2lvbiBmb3IKcHVycG9zZXMgb2Ygc2VjdXJpdHksIGJhY2stdXAgYW5kIHByZXNlcnZhdGlvbi4KCllvdSByZXByZXNlbnQgdGhhdCB0aGUgc3VibWlzc2lvbiBpcyB5b3VyIG9yaWdpbmFsIHdvcmssIGFuZCB0aGF0IHlvdSBoYXZlCnRoZSByaWdodCB0byBncmFudCB0aGUgcmlnaHRzIGNvbnRhaW5lZCBpbiB0aGlzIGxpY2Vuc2UuIFlvdSBhbHNvIHJlcHJlc2VudAp0aGF0IHlvdXIgc3VibWlzc2lvbiBkb2VzIG5vdCwgdG8gdGhlIGJlc3Qgb2YgeW91ciBrbm93bGVkZ2UsIGluZnJpbmdlIHVwb24KYW55b25lJ3MgY29weXJpZ2h0LgoKSWYgdGhlIHN1Ym1pc3Npb24gY29udGFpbnMgbWF0ZXJpYWwgZm9yIHdoaWNoIHlvdSBkbyBub3QgaG9sZCBjb3B5cmlnaHQsCnlvdSByZXByZXNlbnQgdGhhdCB5b3UgaGF2ZSBvYnRhaW5lZCB0aGUgdW5yZXN0cmljdGVkIHBlcm1pc3Npb24gb2YgdGhlCmNvcHlyaWdodCBvd25lciB0byBncmFudCBEU1UgdGhlIHJpZ2h0cyByZXF1aXJlZCBieSB0aGlzIGxpY2Vuc2UsIGFuZCB0aGF0CnN1Y2ggdGhpcmQtcGFydHkgb3duZWQgbWF0ZXJpYWwgaXMgY2xlYXJseSBpZGVudGlmaWVkIGFuZCBhY2tub3dsZWRnZWQKd2l0aGluIHRoZSB0ZXh0IG9yIGNvbnRlbnQgb2YgdGhlIHN1Ym1pc3Npb24uCgpJRiBUSEUgU1VCTUlTU0lPTiBJUyBCQVNFRCBVUE9OIFdPUksgVEhBVCBIQVMgQkVFTiBTUE9OU09SRUQgT1IgU1VQUE9SVEVECkJZIEFOIEFHRU5DWSBPUiBPUkdBTklaQVRJT04gT1RIRVIgVEhBTiBEU1UsIFlPVSBSRVBSRVNFTlQgVEhBVCBZT1UgSEFWRQpGVUxGSUxMRUQgQU5ZIFJJR0hUIE9GIFJFVklFVyBPUiBPVEhFUiBPQkxJR0FUSU9OUyBSRVFVSVJFRCBCWSBTVUNICkNPTlRSQUNUIE9SIEFHUkVFTUVOVC4KCkRTVSB3aWxsIGNsZWFybHkgaWRlbnRpZnkgeW91ciBuYW1lKHMpIGFzIHRoZSBhdXRob3Iocykgb3Igb3duZXIocykgb2YgdGhlCnN1Ym1pc3Npb24sIGFuZCB3aWxsIG5vdCBtYWtlIGFueSBhbHRlcmF0aW9uLCBvdGhlciB0aGFuIGFzIGFsbG93ZWQgYnkgdGhpcwpsaWNlbnNlLCB0byB5b3VyIHN1Ym1pc3Npb24uCg==Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T12:06:40Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Hepatic damage associated with dengue-2 virus replication in liver cells of BALB/c mice
title Hepatic damage associated with dengue-2 virus replication in liver cells of BALB/c mice
spellingShingle Hepatic damage associated with dengue-2 virus replication in liver cells of BALB/c mice
Paes, Marciano Viana
Dengue Virus
Histopathology
In Situ Hybridization
Liver Damage
Mouse Model
Transaminase
Vírus da Dengue
Fígado
Patologia
Transaminases
Hibridização In Situ
title_short Hepatic damage associated with dengue-2 virus replication in liver cells of BALB/c mice
title_full Hepatic damage associated with dengue-2 virus replication in liver cells of BALB/c mice
title_fullStr Hepatic damage associated with dengue-2 virus replication in liver cells of BALB/c mice
title_full_unstemmed Hepatic damage associated with dengue-2 virus replication in liver cells of BALB/c mice
title_sort Hepatic damage associated with dengue-2 virus replication in liver cells of BALB/c mice
author Paes, Marciano Viana
author_facet Paes, Marciano Viana
Lenzi, Henrique Leonel
Nogueira, Ana Cristina Martins
Nuovo, Gerard James
Pinhão, Ângela Teixeira
Mota, Ester Maria
Oliveira, Carlos Alberto Basilio de
Schatzmayr, Hermann
Barth, Ortrud Monika
Alves, Ada Maria de Barcelos
author_role author
author2 Lenzi, Henrique Leonel
Nogueira, Ana Cristina Martins
Nuovo, Gerard James
Pinhão, Ângela Teixeira
Mota, Ester Maria
Oliveira, Carlos Alberto Basilio de
Schatzmayr, Hermann
Barth, Ortrud Monika
Alves, Ada Maria de Barcelos
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Paes, Marciano Viana
Lenzi, Henrique Leonel
Nogueira, Ana Cristina Martins
Nuovo, Gerard James
Pinhão, Ângela Teixeira
Mota, Ester Maria
Oliveira, Carlos Alberto Basilio de
Schatzmayr, Hermann
Barth, Ortrud Monika
Alves, Ada Maria de Barcelos
dc.subject.en.pt_BR.fl_str_mv Dengue Virus
Histopathology
In Situ Hybridization
Liver Damage
Mouse Model
Transaminase
topic Dengue Virus
Histopathology
In Situ Hybridization
Liver Damage
Mouse Model
Transaminase
Vírus da Dengue
Fígado
Patologia
Transaminases
Hibridização In Situ
dc.subject.decs.pt_BR.fl_str_mv Vírus da Dengue
Fígado
Patologia
Transaminases
Hibridização In Situ
description We thank Dr Rita Nogueira (Laboratory of Flaviviruses, Instituto Oswaldo Cruz, Fiocruz) for supplying the dengue 2 isolate and Dr Ricardo Galler (BioManguinhos, Fiocruz) for the anti-DENV-2 serum raised in monkey. We are also grateful for the technical assistance of Aline Vilas Boas Vianna.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2012-01-17T17:45:09Z
dc.date.available.fl_str_mv 2012-01-17T17:45:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PAES, M. V. et al. Hepatic damage associated with dengue-2 virus replication in liver cells of BALB/c mice. Laboratory Investigation. v. 89, n. 10, p. 1140-51, Oct. 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/3737
dc.identifier.issn.none.fl_str_mv 0023-6837
dc.identifier.other.none.fl_str_mv PMID:19721415
identifier_str_mv PAES, M. V. et al. Hepatic damage associated with dengue-2 virus replication in liver cells of BALB/c mice. Laboratory Investigation. v. 89, n. 10, p. 1140-51, Oct. 2009.
0023-6837
PMID:19721415
url https://www.arca.fiocruz.br/handle/icict/3737
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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