Inhibition of the Polymerase Chain Reaction by Sputum Samples from Tuberculosis Patients After Processing Using a Silica-guanidiniumthiocyanate DNA Isolation Procedure

Detalhes bibliográficos
Autor(a) principal: Suffis, Philip
Data de Publicação: 2001
Outros Autores: Vanderborght, Patricia Rosa, Santos, Patricia Barros dos, Correa, Leticia Almeida Pinto, Bravin, Yolanda, Kritski, Afranio Lineu
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44163
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Diagnóstico de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil..
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spelling Suffis, PhilipVanderborght, Patricia RosaSantos, Patricia Barros dosCorrea, Leticia Almeida PintoBravin, YolandaKritski, Afranio Lineu2020-10-24T14:48:16Z2020-10-24T14:48:16Z2001SUFFYS, Philip et al. Inhibition of the Polymerase Chain Reaction by Sputum Samples from Tuberculosis Patients After Processing Using a Silica-guanidiniumthiocyanate DNA Isolation Procedure. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 8, p. 1137-1139, Nov. 2001.0074-0206https://www.arca.fiocruz.br/handle/icict/4416310.1590/S0074-027620010008000191678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.PCREscarroTuberculoseInibiçãoSílica-guanidiniumthiocyanatoPCRSputumTuberculosisInhibitionSilica-guanidiniumthiocyanateEscarroInhibition of the Polymerase Chain Reaction by Sputum Samples from Tuberculosis Patients After Processing Using a Silica-guanidiniumthiocyanate DNA Isolation Procedureinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Diagnóstico de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil..Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Diagnóstico de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil..Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Diagnóstico de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil..Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Diagnóstico de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil..Secretaria Estadual de Saúde do Rio de Janeiro. Laboratório Central Noel Nutels. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Unidade de Pesquisa em Tuberculose. Rio de Janeiro, RJ, Brasil.With the objective to evaluate PCR-mediated detection of Mycobacterium tuberculosis DNA as a diagnostic procedure for diagnosis of tuberculosis in individuals attending ambulatory services in Primary Health Units of the City Tuberculosis Program in Rio de Janeiro, Brazil, their sputum samples were collected and treated with a DNA extraction procedure using silica-guanidiniumthiocyanate. This procedure has been described to be highly efficient for extraction of different kind of nucleic acids from bacteria and clinical samples. Upon comparing PCR results with the number of acid-fast bacilli, no direct relation was observed between the number of bacilli present in the sample and PCR positivity. Part of the processed samples was therefore spiked with pure DNA of M. tuberculosis and inhibition of the PCR reaction was verified in 22 out of 36 (61%) of the samples, demonstrating that the extraction procedure as originally described should not be used for PCR analysis of sputum samples.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44163/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPhilipSuffys_LeticiaCorrea_etal_IOC_2001.pdfPhilipSuffys_LeticiaCorrea_etal_IOC_2001.pdfapplication/pdf39804https://www.arca.fiocruz.br/bitstream/icict/44163/2/PhilipSuffys_LeticiaCorrea_etal_IOC_2001.pdf94f722c6b038fdc07181fbd551d03d7eMD52TEXTPhilipSuffys_LeticiaCorrea_etal_IOC_2001.pdf.txtPhilipSuffys_LeticiaCorrea_etal_IOC_2001.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Inhibition of the Polymerase Chain Reaction by Sputum Samples from Tuberculosis Patients After Processing Using a Silica-guanidiniumthiocyanate DNA Isolation Procedure
title Inhibition of the Polymerase Chain Reaction by Sputum Samples from Tuberculosis Patients After Processing Using a Silica-guanidiniumthiocyanate DNA Isolation Procedure
spellingShingle Inhibition of the Polymerase Chain Reaction by Sputum Samples from Tuberculosis Patients After Processing Using a Silica-guanidiniumthiocyanate DNA Isolation Procedure
Suffis, Philip
PCR
Escarro
Tuberculose
Inibição
Sílica-guanidiniumthiocyanato
PCR
Sputum
Tuberculosis
Inhibition
Silica-guanidiniumthiocyanate
Escarro
title_short Inhibition of the Polymerase Chain Reaction by Sputum Samples from Tuberculosis Patients After Processing Using a Silica-guanidiniumthiocyanate DNA Isolation Procedure
title_full Inhibition of the Polymerase Chain Reaction by Sputum Samples from Tuberculosis Patients After Processing Using a Silica-guanidiniumthiocyanate DNA Isolation Procedure
title_fullStr Inhibition of the Polymerase Chain Reaction by Sputum Samples from Tuberculosis Patients After Processing Using a Silica-guanidiniumthiocyanate DNA Isolation Procedure
title_full_unstemmed Inhibition of the Polymerase Chain Reaction by Sputum Samples from Tuberculosis Patients After Processing Using a Silica-guanidiniumthiocyanate DNA Isolation Procedure
title_sort Inhibition of the Polymerase Chain Reaction by Sputum Samples from Tuberculosis Patients After Processing Using a Silica-guanidiniumthiocyanate DNA Isolation Procedure
author Suffis, Philip
author_facet Suffis, Philip
Vanderborght, Patricia Rosa
Santos, Patricia Barros dos
Correa, Leticia Almeida Pinto
Bravin, Yolanda
Kritski, Afranio Lineu
author_role author
author2 Vanderborght, Patricia Rosa
Santos, Patricia Barros dos
Correa, Leticia Almeida Pinto
Bravin, Yolanda
Kritski, Afranio Lineu
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Suffis, Philip
Vanderborght, Patricia Rosa
Santos, Patricia Barros dos
Correa, Leticia Almeida Pinto
Bravin, Yolanda
Kritski, Afranio Lineu
dc.subject.other.pt_BR.fl_str_mv PCR
Escarro
Tuberculose
Inibição
Sílica-guanidiniumthiocyanato
topic PCR
Escarro
Tuberculose
Inibição
Sílica-guanidiniumthiocyanato
PCR
Sputum
Tuberculosis
Inhibition
Silica-guanidiniumthiocyanate
Escarro
dc.subject.en.pt_BR.fl_str_mv PCR
Sputum
Tuberculosis
Inhibition
Silica-guanidiniumthiocyanate
dc.subject.decs.pt_BR.fl_str_mv Escarro
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Diagnóstico de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil..
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2020-10-24T14:48:16Z
dc.date.available.fl_str_mv 2020-10-24T14:48:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SUFFYS, Philip et al. Inhibition of the Polymerase Chain Reaction by Sputum Samples from Tuberculosis Patients After Processing Using a Silica-guanidiniumthiocyanate DNA Isolation Procedure. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 8, p. 1137-1139, Nov. 2001.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44163
dc.identifier.issn.pt_BR.fl_str_mv 0074-0206
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762001000800019
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv SUFFYS, Philip et al. Inhibition of the Polymerase Chain Reaction by Sputum Samples from Tuberculosis Patients After Processing Using a Silica-guanidiniumthiocyanate DNA Isolation Procedure. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 8, p. 1137-1139, Nov. 2001.
0074-0206
10.1590/S0074-02762001000800019
1678-8060
url https://www.arca.fiocruz.br/handle/icict/44163
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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