Messenger RNA in HeLa cells: an investigation of free and polyribosome-bound cytoplasmic messenger ribonucleoprotein particles by kinetic labelling and electron microscopy

Detalhes bibliográficos
Autor(a) principal: Spohr, Georges
Data de Publicação: 1970
Outros Autores: Granboulan, Nicole, Morel, Carlos, Scherrer, Klaus
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/59130
Resumo: Carlos Morel é o autor vinculado à Fiocruz, mas não consta à informação no documento.
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spelling Spohr, GeorgesGranboulan, NicoleMorel, CarlosScherrer, Klaus2023-06-22T15:05:45Z2023-06-22T15:05:45Z1970SPOHR, G. et al. Messenger RNA in HeLa cells: an investigation of free and polyribosome-bound cytoplasmic messenger ribonucleoprotein particles by kinetic labelling and electron microscopy. European Journal of Biochemistry, v. 17, n. 2, p. 296-318, 1970.1742-4658https://www.arca.fiocruz.br/handle/icict/59130Carlos Morel é o autor vinculado à Fiocruz, mas não consta à informação no documento.Institut Suisse de Recherches Experimentales sur le Cancer. Département de Biologie Moléculaire. Lausanne, SuisseUniversité de Lausanne. Centre de Microscopie Electronique.Villejuif, FranceCentre National de la Recherche Scientifique. Institut de Recherche Scientifique sur le Cancer. Laboratoire de Microscopie Electronique. Villejuif, FranceUniversidade de Brasilia. Faculdade de Ciências Médicas. Brasília, DF, BrasilMessenger RNA (mRNA) and messenger like RNA (mlRNA) were investigated in the cyto- plasm of HeLa cells while ribosomal RNA synthesis was arrested. Under these conditions, func- tional mRNA associated in polyribosomes and cytoplasmic free mlRNA are formed and can be labelled selectively to steady state. All cytoplasmic non-ribosomal RNA sedimenting at more than 6-7 S exists in the form of ribonucleoprotein complexes which pre-exist in the cell, and are stable upon cell lysis, sedimen- tation and (after fixation) CsCl density gradient analysis. The functional, true mRNA is contained in a complex of mRNA and protein which bands in association with ribosomes (e = 1.52 to 1.60 g/cm3) in CsCl density gradients or, released by EDTA, at its own intrinsic density of 1.40-1.88 g/cm3. The cytoplasmic free mlRNA bands as a particle of mlRNA-containing ribo- nucleoprotein at an identical low density. The molecular weight spectrum of mRNA is identical to that of mlRNA and the sedimentation pattern of the mRNA-protein complex released from polyribosomes is similar to that of the free mlRNA - protein complex. The physico-chemical separation of mRNA - and mlRNA - protein complexes allowed us to follow their relative kinetics of synthesis and decay. Each type of ribonucleoprotein obeys a different, strictly time-dependent pattern. Label enters the pool of free mlRNA - protein com- plexes first and may, in a pulse-chase experiment, be partially chased into polyribosomes. At steady state (6 h) 40-60°/, of the labelled RNA remains in the form of free mlRNA-protein particles. These cannot be chased into polyribosomes, the kinetics of mRNA. and mlRNA- protein complexes decay following identical patterns. These findings are in agreement with a model according to which mlRNA from the nucleus first joins the pool of free ribonucleoprotein. Then, the activated mRNA- protein complexes attach to ribosomal subunits and form polyribosomes whereas inactivated mlRNA * protein complexes remain free in the cytoplasm. In order to further strengthen the evidence in favour of the real existence of mRNA- and mlRNA * protein complexes in the cells, the corresponding fractions from sedimentation or CsCl density gradients were observed in the electron microscope. By this method it was possible to see small cytoplasmic particles which have not before been identified. The rounded structures, with diameters ranging from 100 h to 200 8, seem to consist of the coiling of a 35 8 wide pearl- like chain which may also be identsed in polyribosomes. The frequency of these particles is highest in the mlRNA. protein band (e = 1.40-1.48 g/cmS). Thus they may correspond to the mRNA - protein complex. However since they share some morphological features with other known biological structures the evidence is not conclusive.engFEBSPRESSRNA mensageiromicroscopia eletônicaribonucleoproteínaMessenger RNA in HeLa cells: an investigation of free and polyribosome-bound cytoplasmic messenger ribonucleoprotein particles by kinetic labelling and electron microscopyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALEuropean Journal of Biochemistry - December 1970 - Spohr - Messenger RNA in HeLa Cells.pdfEuropean Journal of Biochemistry - December 1970 - Spohr - Messenger RNA in HeLa Cells.pdfapplication/pdf3805053https://www.arca.fiocruz.br/bitstream/icict/59130/4/European%20Journal%20of%20Biochemistry%20-%20December%201970%20-%20Spohr%20-%20Messenger%20RNA%20in%20HeLa%20Cells.pdfb97a9c4e84bcf8100e05d4af6f49d0f0MD54LICENSElicense.txtlicense.txttext/plain; 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dc.title.en_US.fl_str_mv Messenger RNA in HeLa cells: an investigation of free and polyribosome-bound cytoplasmic messenger ribonucleoprotein particles by kinetic labelling and electron microscopy
title Messenger RNA in HeLa cells: an investigation of free and polyribosome-bound cytoplasmic messenger ribonucleoprotein particles by kinetic labelling and electron microscopy
spellingShingle Messenger RNA in HeLa cells: an investigation of free and polyribosome-bound cytoplasmic messenger ribonucleoprotein particles by kinetic labelling and electron microscopy
Spohr, Georges
RNA mensageiro
microscopia eletônica
ribonucleoproteína
title_short Messenger RNA in HeLa cells: an investigation of free and polyribosome-bound cytoplasmic messenger ribonucleoprotein particles by kinetic labelling and electron microscopy
title_full Messenger RNA in HeLa cells: an investigation of free and polyribosome-bound cytoplasmic messenger ribonucleoprotein particles by kinetic labelling and electron microscopy
title_fullStr Messenger RNA in HeLa cells: an investigation of free and polyribosome-bound cytoplasmic messenger ribonucleoprotein particles by kinetic labelling and electron microscopy
title_full_unstemmed Messenger RNA in HeLa cells: an investigation of free and polyribosome-bound cytoplasmic messenger ribonucleoprotein particles by kinetic labelling and electron microscopy
title_sort Messenger RNA in HeLa cells: an investigation of free and polyribosome-bound cytoplasmic messenger ribonucleoprotein particles by kinetic labelling and electron microscopy
author Spohr, Georges
author_facet Spohr, Georges
Granboulan, Nicole
Morel, Carlos
Scherrer, Klaus
author_role author
author2 Granboulan, Nicole
Morel, Carlos
Scherrer, Klaus
author2_role author
author
author
dc.contributor.author.fl_str_mv Spohr, Georges
Granboulan, Nicole
Morel, Carlos
Scherrer, Klaus
dc.subject.other.en_US.fl_str_mv RNA mensageiro
microscopia eletônica
ribonucleoproteína
topic RNA mensageiro
microscopia eletônica
ribonucleoproteína
description Carlos Morel é o autor vinculado à Fiocruz, mas não consta à informação no documento.
publishDate 1970
dc.date.issued.fl_str_mv 1970
dc.date.accessioned.fl_str_mv 2023-06-22T15:05:45Z
dc.date.available.fl_str_mv 2023-06-22T15:05:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SPOHR, G. et al. Messenger RNA in HeLa cells: an investigation of free and polyribosome-bound cytoplasmic messenger ribonucleoprotein particles by kinetic labelling and electron microscopy. European Journal of Biochemistry, v. 17, n. 2, p. 296-318, 1970.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59130
dc.identifier.issn.en_US.fl_str_mv 1742-4658
identifier_str_mv SPOHR, G. et al. Messenger RNA in HeLa cells: an investigation of free and polyribosome-bound cytoplasmic messenger ribonucleoprotein particles by kinetic labelling and electron microscopy. European Journal of Biochemistry, v. 17, n. 2, p. 296-318, 1970.
1742-4658
url https://www.arca.fiocruz.br/handle/icict/59130
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv FEBSPRESS
publisher.none.fl_str_mv FEBSPRESS
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/59130/4/European%20Journal%20of%20Biochemistry%20-%20December%201970%20-%20Spohr%20-%20Messenger%20RNA%20in%20HeLa%20Cells.pdf
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