The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/33639 |
Resumo: | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil. |
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Alves, Ceres LucianaRepolês, Bruno MarçalSilva, Marcelo Santos daMendes, Isabela CecíliaMarin, Paula AndreaAguiar, Pedro Henrique NascimentoSantos, Selma da SilvaFranco, Glória ReginaMacedo, Andréa MaraPena, Sérgio Danilo JunhoAndrade, Luciana de OliveiraGuarneri, Alessandra AparecidaTahara, Erich BirelliElias, Maria CarolinaMachado, Carlos Renato2019-06-24T16:57:35Z2019-06-24T16:57:35Z2018ALVES, Ceres Luciana et al. The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi. Scientific Reports, v. 8, p. 1-12, 2018.2045-2322https://www.arca.fiocruz.br/handle/icict/3363910.1038/s41598-018-31541-zengNature ResearchTrypanosoma cruziTrypanosoma cruziThe recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular. Laboratório Especial de Ciclo Celular. São Paulo, SP, Brazil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular. Laboratório Especial de Ciclo Celular. São Paulo, SP, Brazil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG Brazil.Universidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG Brazil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular. Laboratório Especial de Ciclo Celular. São Paulo, SP, Brazil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Detection of genetic exchange has been a limiting factor to deepen the knowledge on the mechanisms by which Trypanosoma cruzi is able to generate progeny and genetic diversity. Here we show that incorporation of halogenated thymidine analogues, followed by immunostaining, is a reliable method not only to detect T. cruzi fused-cell hybrids, but also to quantify their percentage in populations of this parasite. Through this approach, we were able to detect and quantify fused-cell hybrids of T. cruzi clones CL Brener and Y. Given the increased detection of fused-cell hybrids in naturally-occurring hybrid CL Brener strain, which displays increased levels of RAD51 and BRCA2 transcripts, we further investigated the role of Rad51 – a recombinase involved in homologous recombination – in the process of genetic exchange. We also verified that the detection of fused-cell hybrids in T. cruzi overexpressing RAD51 is increased when compared to wild-type cells, suggesting a key role for Rad51 either in the formation or in the stabilization of fused-cell hybrids in this organism.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/33639/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALThe recombinase Rad51.pdfThe recombinase Rad51.pdfapplication/pdf7462652https://www.arca.fiocruz.br/bitstream/icict/33639/2/The%20recombinase%20Rad51.pdfd2c0b2a73c5ee203c2681bcf6f90618eMD52TEXTThe recombinase Rad51.pdf.txtThe recombinase Rad51.pdf.txtExtracted texttext/plain559https://www.arca.fiocruz.br/bitstream/icict/33639/3/The%20recombinase%20Rad51.pdf.txtaa69fcf2f8ea54b70784b5737114c772MD53icict/336392019-06-25 21:40:16.737oai:www.arca.fiocruz.br:icict/33639Q0VTU8ODTyBOw4NPIEVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUw0KDQpOdXppYSBTYW50b3MsIENQRjogNjM1LjA2NC41OTYtMDAsIHZpbmN1bGFkbyBhIENQcVJSIC0gQ2VudHJvIGRlIFBlc3F1aXNhcyBSZW7DqSBSYWNob3UKCkFvIGFjZWl0YXIgb3MgVEVSTU9TIGUgQ09OREnDh8OVRVMgZGVzdGEgQ0VTU8ODTywgbyBBVVRPUiBlL291IFRJVFVMQVIgZGUgZGlyZWl0b3MKYXV0b3JhaXMgc29icmUgYSBPQlJBIGRlIHF1ZSB0cmF0YSBlc3RlIGRvY3VtZW50bzoKCigxKSBDRURFIGUgVFJBTlNGRVJFLCB0b3RhbCBlIGdyYXR1aXRhbWVudGUsIMOgIEZJT0NSVVogLSBGVU5EQcOHw4NPIE9TV0FMRE8gQ1JVWiwgZW0KY2Fyw6F0ZXIgcGVybWFuZW50ZSwgaXJyZXZvZ8OhdmVsIGUgTsODTyBFWENMVVNJVk8sIHRvZG9zIG9zIGRpcmVpdG9zIHBhdHJpbW9uaWFpcyBOw4NPCkNPTUVSQ0lBSVMgZGUgdXRpbGl6YcOnw6NvIGRhIE9CUkEgYXJ0w61zdGljYSBlL291IGNpZW50w61maWNhIGluZGljYWRhIGFjaW1hLCBpbmNsdXNpdmUgb3MgZGlyZWl0b3MKZGUgdm96IGUgaW1hZ2VtIHZpbmN1bGFkb3Mgw6AgT0JSQSwgZHVyYW50ZSB0b2RvIG8gcHJhem8gZGUgZHVyYcOnw6NvIGRvcyBkaXJlaXRvcyBhdXRvcmFpcywgZW0KcXVhbHF1ZXIgaWRpb21hIGUgZW0gdG9kb3Mgb3MgcGHDrXNlczsKCigyKSBBQ0VJVEEgcXVlIGEgY2Vzc8OjbyB0b3RhbCBuw6NvIGV4Y2x1c2l2YSwgcGVybWFuZW50ZSBlIGlycmV2b2fDoXZlbCBkb3MgZGlyZWl0b3MgYXV0b3JhaXMKcGF0cmltb25pYWlzIG7Do28gY29tZXJjaWFpcyBkZSB1dGlsaXphw6fDo28gZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvIGluY2x1aSwgZXhlbXBsaWZpY2F0aXZhbWVudGUsCm9zIGRpcmVpdG9zIGRlIGRpc3BvbmliaWxpemHDp8OjbyBlIGNvbXVuaWNhw6fDo28gcMO6YmxpY2EgZGEgT0JSQSwgZW0gcXVhbHF1ZXIgbWVpbyBvdSB2ZcOtY3VsbywKaW5jbHVzaXZlIGVtIFJlcG9zaXTDs3Jpb3MgRGlnaXRhaXMsIGJlbSBjb21vIG9zIGRpcmVpdG9zIGRlIHJlcHJvZHXDp8OjbywgZXhpYmnDp8OjbywgZXhlY3XDp8OjbywKZGVjbGFtYcOnw6NvLCByZWNpdGHDp8OjbywgZXhwb3Npw6fDo28sIGFycXVpdmFtZW50bywgaW5jbHVzw6NvIGVtIGJhbmNvIGRlIGRhZG9zLCBwcmVzZXJ2YcOnw6NvLCBkaWZ1c8OjbywKZGlzdHJpYnVpw6fDo28sIGRpdnVsZ2HDp8OjbywgZW1wcsOpc3RpbW8sIHRyYWR1w6fDo28sIGR1YmxhZ2VtLCBsZWdlbmRhZ2VtLCBpbmNsdXPDo28gZW0gbm92YXMgb2JyYXMgb3UKY29sZXTDom5lYXMsIHJldXRpbGl6YcOnw6NvLCBlZGnDp8OjbywgcHJvZHXDp8OjbyBkZSBtYXRlcmlhbCBkaWTDoXRpY28gZSBjdXJzb3Mgb3UgcXVhbHF1ZXIgZm9ybWEgZGUKdXRpbGl6YcOnw6NvIG7Do28gY29tZXJjaWFsOwoKKDMpIFJFQ09OSEVDRSBxdWUgYSBjZXNzw6NvIGFxdWkgZXNwZWNpZmljYWRhIGNvbmNlZGUgw6AgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETwpDUlVaIG8gZGlyZWl0byBkZSBhdXRvcml6YXIgcXVhbHF1ZXIgcGVzc29hIOKAkyBmw61zaWNhIG91IGp1csOtZGljYSwgcMO6YmxpY2Egb3UgcHJpdmFkYSwgbmFjaW9uYWwgb3UKZXN0cmFuZ2VpcmEg4oCTIGEgYWNlc3NhciBlIHV0aWxpemFyIGFtcGxhbWVudGUgYSBPQlJBLCBzZW0gZXhjbHVzaXZpZGFkZSwgcGFyYSBxdWFpc3F1ZXIKZmluYWxpZGFkZXMgbsOjbyBjb21lcmNpYWlzOwoKKDQpIERFQ0xBUkEgcXVlIGEgb2JyYSDDqSBjcmlhw6fDo28gb3JpZ2luYWwgZSBxdWUgw6kgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBhcXVpIGNlZGlkb3MgZSBhdXRvcml6YWRvcywKcmVzcG9uc2FiaWxpemFuZG8tc2UgaW50ZWdyYWxtZW50ZSBwZWxvIGNvbnRlw7pkbyBlIG91dHJvcyBlbGVtZW50b3MgcXVlIGZhemVtIHBhcnRlIGRhIE9CUkEsCmluY2x1c2l2ZSBvcyBkaXJlaXRvcyBkZSB2b3ogZSBpbWFnZW0gdmluY3VsYWRvcyDDoCBPQlJBLCBvYnJpZ2FuZG8tc2UgYSBpbmRlbml6YXIgdGVyY2Vpcm9zIHBvcgpkYW5vcywgYmVtIGNvbW8gaW5kZW5pemFyIGUgcmVzc2FyY2lyIGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIGRlCmV2ZW50dWFpcyBkZXNwZXNhcyBxdWUgdmllcmVtIGEgc3Vwb3J0YXIsIGVtIHJhesOjbyBkZSBxdWFscXVlciBvZmVuc2EgYSBkaXJlaXRvcyBhdXRvcmFpcyBvdQpkaXJlaXRvcyBkZSB2b3ogb3UgaW1hZ2VtLCBwcmluY2lwYWxtZW50ZSBubyBxdWUgZGl6IHJlc3BlaXRvIGEgcGzDoWdpbyBlIHZpb2xhw6fDtWVzIGRlIGRpcmVpdG9zOwoKKDUpIEFGSVJNQSBxdWUgY29uaGVjZSBhIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08KT1NXQUxETyBDUlVaIGUgYXMgZGlyZXRyaXplcyBwYXJhIG8gZnVuY2lvbmFtZW50byBkbyByZXBvc2l0w7NyaW8gaW5zdGl0dWNpb25hbCBBUkNBLgoKQSBQb2zDrXRpY2EgSW5zdGl0dWNpb25hbCBkZSBBY2Vzc28gQWJlcnRvIGRhIEZJT0NSVVogLSBGVU5EQcOHw4NPIE9TV0FMRE8gQ1JVWiByZXNlcnZhCmV4Y2x1c2l2YW1lbnRlIGFvIEFVVE9SIG9zIGRpcmVpdG9zIG1vcmFpcyBlIG9zIHVzb3MgY29tZXJjaWFpcyBzb2JyZSBhcyBvYnJhcyBkZSBzdWEgYXV0b3JpYQplL291IHRpdHVsYXJpZGFkZSwgc2VuZG8gb3MgdGVyY2Vpcm9zIHVzdcOhcmlvcyByZXNwb25zw6F2ZWlzIHBlbGEgYXRyaWJ1acOnw6NvIGRlIGF1dG9yaWEgZSBtYW51dGVuw6fDo28KZGEgaW50ZWdyaWRhZGUgZGEgT0JSQSBlbSBxdWFscXVlciB1dGlsaXphw6fDo28uCgpBIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaCnJlc3BlaXRhIG9zIGNvbnRyYXRvcyBlIGFjb3Jkb3MgcHJlZXhpc3RlbnRlcyBkb3MgQXV0b3JlcyBjb20gdGVyY2Vpcm9zLCBjYWJlbmRvIGFvcyBBdXRvcmVzCmluZm9ybWFyIMOgIEluc3RpdHVpw6fDo28gYXMgY29uZGnDp8O1ZXMgZSBvdXRyYXMgcmVzdHJpw6fDtWVzIGltcG9zdGFzIHBvciBlc3RlcyBpbnN0cnVtZW50b3MuCg==Repositório 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dc.title.pt_BR.fl_str_mv |
The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi |
title |
The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi |
spellingShingle |
The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi Alves, Ceres Luciana Trypanosoma cruzi Trypanosoma cruzi |
title_short |
The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi |
title_full |
The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi |
title_fullStr |
The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi |
title_full_unstemmed |
The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi |
title_sort |
The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi |
author |
Alves, Ceres Luciana |
author_facet |
Alves, Ceres Luciana Repolês, Bruno Marçal Silva, Marcelo Santos da Mendes, Isabela Cecília Marin, Paula Andrea Aguiar, Pedro Henrique Nascimento Santos, Selma da Silva Franco, Glória Regina Macedo, Andréa Mara Pena, Sérgio Danilo Junho Andrade, Luciana de Oliveira Guarneri, Alessandra Aparecida Tahara, Erich Birelli Elias, Maria Carolina Machado, Carlos Renato |
author_role |
author |
author2 |
Repolês, Bruno Marçal Silva, Marcelo Santos da Mendes, Isabela Cecília Marin, Paula Andrea Aguiar, Pedro Henrique Nascimento Santos, Selma da Silva Franco, Glória Regina Macedo, Andréa Mara Pena, Sérgio Danilo Junho Andrade, Luciana de Oliveira Guarneri, Alessandra Aparecida Tahara, Erich Birelli Elias, Maria Carolina Machado, Carlos Renato |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Alves, Ceres Luciana Repolês, Bruno Marçal Silva, Marcelo Santos da Mendes, Isabela Cecília Marin, Paula Andrea Aguiar, Pedro Henrique Nascimento Santos, Selma da Silva Franco, Glória Regina Macedo, Andréa Mara Pena, Sérgio Danilo Junho Andrade, Luciana de Oliveira Guarneri, Alessandra Aparecida Tahara, Erich Birelli Elias, Maria Carolina Machado, Carlos Renato |
dc.subject.other.pt_BR.fl_str_mv |
Trypanosoma cruzi |
topic |
Trypanosoma cruzi Trypanosoma cruzi |
dc.subject.en.pt_BR.fl_str_mv |
Trypanosoma cruzi |
description |
Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2019-06-24T16:57:35Z |
dc.date.available.fl_str_mv |
2019-06-24T16:57:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ALVES, Ceres Luciana et al. The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi. Scientific Reports, v. 8, p. 1-12, 2018. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/33639 |
dc.identifier.issn.pt_BR.fl_str_mv |
2045-2322 |
dc.identifier.doi.none.fl_str_mv |
10.1038/s41598-018-31541-z |
identifier_str_mv |
ALVES, Ceres Luciana et al. The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi. Scientific Reports, v. 8, p. 1-12, 2018. 2045-2322 10.1038/s41598-018-31541-z |
url |
https://www.arca.fiocruz.br/handle/icict/33639 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Nature Research |
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Nature Research |
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