Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/12609 |
Resumo: | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil |
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Faria, Angélica RosaVeloso, Luciano de CastroVital, Wendel CouraReis, Alexandre BarbosaDamasceno, Leonardo MirandaGazzinelli, Ricardo Tostes2016-01-25T17:58:00Z2016-01-25T17:58:00Z2015FARIA, Angélica Rosa et al. Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs. PLoS Negl Trop Dis., vol. 9, n. 1, p. e3429, 20151935-2735https://www.arca.fiocruz.br/handle/icict/1260910.1371/journal.pntd.0003429engPublic Library of ScienceNovel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Ouro Preto. Núcleo de Pesquisa em Ciências Biológicas. Ouro Preto, MG, BrasilUniversidade Federal de Ouro Preto. Núcleo de Pesquisa em Ciências Biológicas. Ouro Preto, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil/University of Massachusetts Medical School. Division of Infectious Diseases and Immunology. Worcester, MA, United States of AmericaUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilBACKGROUND: Visceral leishmaniasis is the most severe form of leishmaniasis. Worldwide, approximately 20% of zoonotic human visceral leishmaniasis is caused by Leishmania infantum, also known as Leishmania chagasi in Latin America. Current diagnostic methods are not accurate enough to identify Leishmania-infected animals and may compromise the effectiveness of disease control. Therefore, we aimed to produce and test two recombinant multiepitope proteins as a means to improve and increase accuracy in the diagnosis of canine visceral leishmaniasis (CVL). METHODOLOGY/PRINCIPAL FINDINGS: Ten antigenic peptides were identified by CVL ELISA in previous work. In the current proposal, the coding sequences of these ten peptides were assembled into a synthetic gene. Furthermore, other twenty peptides were selected from work by our group where good B and T cell epitopes were mapped. The coding sequences of these peptides were also assembled into a synthetic gene. Both genes have been cloned and expressed in Escherichia coli, producing two multiepitope recombinant proteins, PQ10 and PQ20. These antigens have been used in CVL ELISA and were able to identify asymptomatic dogs (80%) more effectively than EIE-LVC kit, produced by Bio-Manguinhos (0%) and DPP kit (10%). Moreover, our recombinant proteins presented an early detection (before PCR) of infected dogs, with positivities ranging from 23% to 65%, depending on the phase of infection in which sera were acquired. CONCLUSIONS/SIGNIFICANCE: Our study shows that ELISA using the multiepitope proteins PQ10 and PQ20 has great potential in early CVL diagnosis. The use of these proteins in other methodologies, such as immunochromatographic tests, could be beneficial mainly for the detection of asymptomatic dogs.leishmaniasisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12609/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALNovel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs.pdfNovel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs.pdfapplication/pdf5117566https://www.arca.fiocruz.br/bitstream/icict/12609/2/Novel%20Recombinant%20Multiepitope%20Proteins%20for%20the%20Diagnosis%20of%20Asymptomatic%20Leishmania%20infantum-Infected%20Dogs.pdf3071ed2c6334cadac72f182d860a0b95MD52TEXTNovel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs.pdf.txtNovel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs.pdf.txtExtracted texttext/plain54https://www.arca.fiocruz.br/bitstream/icict/12609/3/Novel%20Recombinant%20Multiepitope%20Proteins%20for%20the%20Diagnosis%20of%20Asymptomatic%20Leishmania%20infantum-Infected%20Dogs.pdf.txt32ceaf957275278a8f3f2b27bb2efbefMD53icict/126092019-06-19 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dc.title.pt_BR.fl_str_mv |
Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs |
title |
Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs |
spellingShingle |
Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs Faria, Angélica Rosa leishmaniasis |
title_short |
Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs |
title_full |
Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs |
title_fullStr |
Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs |
title_full_unstemmed |
Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs |
title_sort |
Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs |
author |
Faria, Angélica Rosa |
author_facet |
Faria, Angélica Rosa Veloso, Luciano de Castro Vital, Wendel Coura Reis, Alexandre Barbosa Damasceno, Leonardo Miranda Gazzinelli, Ricardo Tostes |
author_role |
author |
author2 |
Veloso, Luciano de Castro Vital, Wendel Coura Reis, Alexandre Barbosa Damasceno, Leonardo Miranda Gazzinelli, Ricardo Tostes |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Faria, Angélica Rosa Veloso, Luciano de Castro Vital, Wendel Coura Reis, Alexandre Barbosa Damasceno, Leonardo Miranda Gazzinelli, Ricardo Tostes |
dc.subject.en.pt_BR.fl_str_mv |
leishmaniasis |
topic |
leishmaniasis |
description |
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2016-01-25T17:58:00Z |
dc.date.available.fl_str_mv |
2016-01-25T17:58:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FARIA, Angélica Rosa et al. Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs. PLoS Negl Trop Dis., vol. 9, n. 1, p. e3429, 2015 |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/12609 |
dc.identifier.issn.none.fl_str_mv |
1935-2735 |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pntd.0003429 |
identifier_str_mv |
FARIA, Angélica Rosa et al. Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs. PLoS Negl Trop Dis., vol. 9, n. 1, p. e3429, 2015 1935-2735 10.1371/journal.pntd.0003429 |
url |
https://www.arca.fiocruz.br/handle/icict/12609 |
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eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Public Library of Science |
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Public Library of Science |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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