Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7442 |
Resumo: | Universidade Federal de Minas Gerais. Department of Biochemistry-Immunology. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Medical Center. Belo Horizonte, MG, Brasil |
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Bottrel, R. LDutra, Walderez OrnelasMartins, Fabrícia AndradeGontijo, BCarvalho Filho, Edgar MarcelinoBarral Netto, ManoelBarral, Aldina Maria PradoAlmeida, Roque Pacheco deMayrink, WilsonLocksley, RGollob, Kenneth J2014-03-25T12:59:44Z2014-03-25T12:59:44Z2001BOTTREL, R. L. et al. Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK. Infection and Immunity, v. 69, n. 5, p.3232-3239, 2001.0019-9567https://www.arca.fiocruz.br/handle/icict/744210.1128/IAI.69.5.3232–3239.2001engAmerican Society for MicrobiologyFlow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACKinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Department of Biochemistry-Immunology. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Medical Center. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Department of Morphology. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Medical Center. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Department of Biochemistry-Immunology. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Medical Center. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Medical Center. Belo Horizonte, MG, BrasilUniversidade Federal da Bahia. Medical Center. Salvador, BA, BrasilUniversidade Federal da Bahia. Medical Center. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Medical Center. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Medical Center. Salvador, BA, BrasilUniversidade Federal de Minas Gerais. Department of Parasitology. Belo Horizonte, MG, BrasilUCSF Medical Center. San Francisco, CaliforniaUniversidade Federal de Minas Gerais. Department of Biochemistry-Immunology. Belo Horizonte, MG, BrasilLeishmaniasis, caused by infection with the protozoan parasite Leishmania, affects millions of individuals worldwide, causing serious morbidity and mortality. This study directly determined the frequency of cells producing key immunoregulatory cytokines in response to the recombinant antigen Leishmania homolog of receptors for activated kinase C (LACK) and soluble leishmania antigen (SLA), and it determined relative contributions of these antigens to the overall cytokine profile in individuals infected for the first time with Leishmania braziliensis. All individuals presented with the cutaneous clinical form of leishmaniasis and were analyzed for proliferative responses to LACK antigen and SLA, frequency of lymphocyte subpopulations (analyzed ex vivo), and antigen-induced (LACK and SLA) cytokine production at the single-cell level (determined by flow cytometry). The following were determined. (i) The Th1-type response previously seen in patients with cutaneous leishmaniasis is due to gamma interferon (IFN-g) production by several different sources, listed in order of contribution: CD41 T lymphocytes, CD42, CD82 lymphocytes, and CD81 T lymphocytes. (ii) SLA induced a higher frequency of lymphocytes producing IFN-g and tumor necrosis factor alpha (TNF-a) than did LACK. (iii) LACK induced an activation of monocyte populations as reflected by an increased percentage of CD14-positive cells. (iv) Neither SLA nor LACK induced detectable frequencies of cells producing interleukin-4 (IL-4) or IL-5. These data demonstrated a multifaceted immune response to SLA in human leishmaniasis involving Th1 CD41 T lymphocytes (IFN-g1 and IL-102/IL-42), Tc1 CD81 T cells (IFN-g1, and IL-102/IL-42), and a high frequency of TNF-a-producing lymphocytes. Moreover, it was determined that the recombinant antigen LACK acts as a weak inducer of Th1-type lymphocyte responses compared to SLA.Antígenos de Protozoários/imunologiaCitocinas/biossínteseCitometria de FluxoLeishmaniose Cutânea/imunologiaProteínas de Protozoários/imunologiaHumanosCélulas Th1/imunologiaInterferon gama/biossínteseInterleucina-10/biossínteseProteínas Recombinantes/imunologiaFator de Necrose Tumoral alfa/biossínteseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBottrel R L Flow Cytometric....pdfBottrel R L Flow Cytometric....pdfapplication/pdf154360https://www.arca.fiocruz.br/bitstream/icict/7442/1/Bottrel%20R%20L%20Flow%20Cytometric....pdfbff78f52e426df1d05e0ec03222f782aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7442/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTBottrel R L Flow Cytometric....pdf.txtBottrel R L Flow Cytometric....pdf.txtExtracted texttext/plain39950https://www.arca.fiocruz.br/bitstream/icict/7442/5/Bottrel%20R%20L%20Flow%20Cytometric....pdf.txtb139c1dcd0296d69a12f7f7a1da3ba15MD55THUMBNAILBottrel R L Flow Cytometric....pdf.jpgBottrel R L Flow Cytometric....pdf.jpgGenerated Thumbnailimage/jpeg2230https://www.arca.fiocruz.br/bitstream/icict/7442/4/Bottrel%20R%20L%20Flow%20Cytometric....pdf.jpg5e482c03c52f3063899547a168a0b8e0MD54icict/74422023-03-15 14:33:56.626oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:56Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK |
title |
Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK |
spellingShingle |
Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK Bottrel, R. L Antígenos de Protozoários/imunologia Citocinas/biossíntese Citometria de Fluxo Leishmaniose Cutânea/imunologia Proteínas de Protozoários/imunologia Humanos Células Th1/imunologia Interferon gama/biossíntese Interleucina-10/biossíntese Proteínas Recombinantes/imunologia Fator de Necrose Tumoral alfa/biossíntese |
title_short |
Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK |
title_full |
Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK |
title_fullStr |
Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK |
title_full_unstemmed |
Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK |
title_sort |
Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK |
author |
Bottrel, R. L |
author_facet |
Bottrel, R. L Dutra, Walderez Ornelas Martins, Fabrícia Andrade Gontijo, B Carvalho Filho, Edgar Marcelino Barral Netto, Manoel Barral, Aldina Maria Prado Almeida, Roque Pacheco de Mayrink, Wilson Locksley, R Gollob, Kenneth J |
author_role |
author |
author2 |
Dutra, Walderez Ornelas Martins, Fabrícia Andrade Gontijo, B Carvalho Filho, Edgar Marcelino Barral Netto, Manoel Barral, Aldina Maria Prado Almeida, Roque Pacheco de Mayrink, Wilson Locksley, R Gollob, Kenneth J |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Bottrel, R. L Dutra, Walderez Ornelas Martins, Fabrícia Andrade Gontijo, B Carvalho Filho, Edgar Marcelino Barral Netto, Manoel Barral, Aldina Maria Prado Almeida, Roque Pacheco de Mayrink, Wilson Locksley, R Gollob, Kenneth J |
dc.subject.decs.pt_BR.fl_str_mv |
Antígenos de Protozoários/imunologia Citocinas/biossíntese Citometria de Fluxo Leishmaniose Cutânea/imunologia Proteínas de Protozoários/imunologia Humanos Células Th1/imunologia Interferon gama/biossíntese Interleucina-10/biossíntese Proteínas Recombinantes/imunologia Fator de Necrose Tumoral alfa/biossíntese |
topic |
Antígenos de Protozoários/imunologia Citocinas/biossíntese Citometria de Fluxo Leishmaniose Cutânea/imunologia Proteínas de Protozoários/imunologia Humanos Células Th1/imunologia Interferon gama/biossíntese Interleucina-10/biossíntese Proteínas Recombinantes/imunologia Fator de Necrose Tumoral alfa/biossíntese |
description |
Universidade Federal de Minas Gerais. Department of Biochemistry-Immunology. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Medical Center. Belo Horizonte, MG, Brasil |
publishDate |
2001 |
dc.date.issued.fl_str_mv |
2001 |
dc.date.accessioned.fl_str_mv |
2014-03-25T12:59:44Z |
dc.date.available.fl_str_mv |
2014-03-25T12:59:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BOTTREL, R. L. et al. Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK. Infection and Immunity, v. 69, n. 5, p.3232-3239, 2001. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/7442 |
dc.identifier.issn.none.fl_str_mv |
0019-9567 |
dc.identifier.doi.none.fl_str_mv |
10.1128/IAI.69.5.3232–3239.2001 |
identifier_str_mv |
BOTTREL, R. L. et al. Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK. Infection and Immunity, v. 69, n. 5, p.3232-3239, 2001. 0019-9567 10.1128/IAI.69.5.3232–3239.2001 |
url |
https://www.arca.fiocruz.br/handle/icict/7442 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
American Society for Microbiology |
publisher.none.fl_str_mv |
American Society for Microbiology |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Fundação Oswaldo Cruz (FIOCRUZ) |
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FIOCRUZ |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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Repositório Institucional da FIOCRUZ (ARCA) |
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