Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Columbian South America times

Detalhes bibliográficos
Autor(a) principal: Leles, Daniela
Data de Publicação: 2008
Outros Autores: Araújo, Adauto, Ferreira, Luiz Fernando, Vicente, Ana Carolina Paulo, Iñiguez, Alena Mayo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32020
Resumo: Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro. RJ, Brasil
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spelling Leles, DanielaAraújo, AdautoFerreira, Luiz FernandoVicente, Ana Carolina PauloIñiguez, Alena Mayo2019-03-09T18:52:30Z2019-03-09T18:52:30Z2008LELES, Daniela et al. Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Columbian South America times. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 1, p. 106-108, Feb. 2008.0074-0276https://www.arca.fiocruz.br/handle/icict/3202010.1590/S0074-027620080050000041678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.AscarisPaleodistribuiçãoDiagnóstico paleoparasitológico molecularAscarisMolecular paleoparasitological diagnosisPaleodistributionMolecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Columbian South America timesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro. RJ, BrasilFundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro. RJ, BrasilFundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro. RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Paleoparasitological studies using microscopy showed that Ascaris and Trichuris trichiura are the human intestinal parasites most found in archaeological sites. However, in pre-Columbian South American archaeological sites, Ascaris is rare. In this work we standardized a molecular methodology for Ascaris diagnosis directly from ancient DNA retrieved from coprolites. Using cythochrome b gene (142 bp) target, ancient DNA sequences were retrieved from South American samples, negative by microscopy. Moreover, the methodology applied was sensitive enough to detect ancient DNA extracted from 30 Ascaris eggs from an European coprolite. These results revealed a new scenery for the paleodistribution of Ascaris in South America.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/32020/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAnaCVicente_AlenaMayo_etal_IOC_2008.pdfAnaCVicente_AlenaMayo_etal_IOC_2008.pdfapplication/pdf512932https://www.arca.fiocruz.br/bitstream/icict/32020/2/AnaCVicente_AlenaMayo_etal_IOC_2008.pdf55ba4346c10b536b21e2dbfe672b1085MD52TEXTAnaCVicente_AlenaMayo_etal_IOC_2008.pdf.txtAnaCVicente_AlenaMayo_etal_IOC_2008.pdf.txtExtracted texttext/plain17002https://www.arca.fiocruz.br/bitstream/icict/32020/3/AnaCVicente_AlenaMayo_etal_IOC_2008.pdf.txtc6280aea9c21e954f54cdb4792b022fcMD53icict/320202022-06-24 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dc.title.pt_BR.fl_str_mv Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Columbian South America times
title Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Columbian South America times
spellingShingle Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Columbian South America times
Leles, Daniela
Ascaris
Paleodistribuição
Diagnóstico paleoparasitológico molecular
Ascaris
Molecular paleoparasitological diagnosis
Paleodistribution
title_short Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Columbian South America times
title_full Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Columbian South America times
title_fullStr Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Columbian South America times
title_full_unstemmed Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Columbian South America times
title_sort Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Columbian South America times
author Leles, Daniela
author_facet Leles, Daniela
Araújo, Adauto
Ferreira, Luiz Fernando
Vicente, Ana Carolina Paulo
Iñiguez, Alena Mayo
author_role author
author2 Araújo, Adauto
Ferreira, Luiz Fernando
Vicente, Ana Carolina Paulo
Iñiguez, Alena Mayo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Leles, Daniela
Araújo, Adauto
Ferreira, Luiz Fernando
Vicente, Ana Carolina Paulo
Iñiguez, Alena Mayo
dc.subject.other.pt_BR.fl_str_mv Ascaris
Paleodistribuição
Diagnóstico paleoparasitológico molecular
topic Ascaris
Paleodistribuição
Diagnóstico paleoparasitológico molecular
Ascaris
Molecular paleoparasitological diagnosis
Paleodistribution
dc.subject.en.pt_BR.fl_str_mv Ascaris
Molecular paleoparasitological diagnosis
Paleodistribution
description Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro. RJ, Brasil
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2019-03-09T18:52:30Z
dc.date.available.fl_str_mv 2019-03-09T18:52:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv LELES, Daniela et al. Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Columbian South America times. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 1, p. 106-108, Feb. 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32020
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/S0074-02762008005000004
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv LELES, Daniela et al. Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Columbian South America times. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 1, p. 106-108, Feb. 2008.
0074-0276
10.1590/S0074-02762008005000004
1678-8060
url https://www.arca.fiocruz.br/handle/icict/32020
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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