The Threat Called Candida haemulonii Species Complex in Rio de Janeiro State, Brazil: Focus on Antifungal Resistance and Virulence Attributes
| Autor(a) principal: | |
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| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/55519 |
Resumo: | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil. |
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Ramos, Lívia S.Figueiredo-Carvalho, Maria Helena G.Silva, Laura N.Siqueira, Nahyara L. M.Lima, Joice C.Oliveira, Samuel S.Almeida-Paes, RodrigoZancopé-Oliveira, Rosely MariaAzevedo, Fabio S.Ferreira, Adriana L. P.Branquinha, Marta H.Santos, André L. S.2022-11-07T19:01:35Z2022-11-07T19:01:35Z2022RAMOS, Lívia S. et al. The Threat Called Candida haemulonii Species Complex in Rio de Janeiro State, Brazil: Focus on Antifungal Resistance and Virulence Attributes. Journal of fungi, v. 8, v. 6, p. 1-19, 20222309-608Xhttps://www.arca.fiocruz.br/handle/icict/5551910.3390/jof8060574engMDPIThe Threat Called Candida haemulonii Species Complex in Rio de Janeiro State, Brazil: Focus on Antifungal Resistance and Virulence Attributesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil / Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Laboratório Diagnóstico da América SA. Duque de Caxias, RJ, Brasil.Laboratório Diagnóstico da América SA. Duque de Caxias, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil / Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Química. Programa de Pós-Graduação em Bioquímica. Rio de Janeiro, RJ, Brasil.Although considered rare, the emergent Candida haemulonii species complex, formed by C. haemulonii sensu stricto (Ch), C. duobushaemulonii (Cd) and C. haemulonii var. vulnera (Chv), is highlighted due to its profile of increased resistance to the available antifungal drugs. In the present work, 25 clinical isolates, recovered from human infections during 2011-2020 and biochemically identified by automated system as C. haemulonii, were initially assessed by molecular methods (amplification and sequencing of ITS1-5.8S-ITS2 gene) for precise species identification. Subsequently, the antifungal susceptibility of planktonic cells, biofilm formation and susceptibility of biofilms to antifungal drugs and the secretion of key molecules, such as hydrolytic enzymes, hemolysins and siderophores, were evaluated by classical methodologies. Our results revealed that 7 (28%) isolates were molecularly identified as Ch, 7 (28%) as Chv and 11 (44%) as Cd. Sixteen (64%) fungal isolates were recovered from blood. Regarding the antifungal susceptibility test, the planktonic cells were resistant to (i) fluconazole (100% of Ch and Chv, and 72.7% of Cd isolates), itraconazole and voriconazole (85.7% of Ch and Chv, and 72.7% of Cd isolates); (ii) no breakpoints were defined for posaconazole, but high MICs were observed for 85.7% of Ch and Chv, and 72.7% of Cd isolates; (iii) all isolates were resistant to amphotericin B; and (iv) all isolates were susceptible to echinocandins (except for one isolate of Cd) and to flucytosine (except for two isolates of Cd). Biofilm is a well-known virulence and resistant structure in Candida species, including the C. haemulonii complex. Herein, we showed that all isolates were able to form viable biofilms over a polystyrene surface. Moreover, the mature biofilms formed by the C. haemulonii species complex presented a higher antifungal-resistant profile than their planktonic counterparts. Secreted molecules associated with virulence were also detected in our fungal collection: 100% of the isolates yielded aspartic proteases, hemolysins and siderophores as well as phospholipase (92%), esterase (80%), phytase (80%), and caseinase (76%) activities. Our results reinforce the multidrug resistance profile of the C. haemulonii species complex, including Brazilian clinical isolates, as well as their ability to produce important virulence attributes such as biofilms and different classes of hydrolytic enzymes, hemolysins and siderophores, which typically present a strain-dependent profile.Candida haemulonii complexAntifungal resistanceBiofilm formationHemolysinsHydrolytic enzymesSiderophoresinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/55519/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALThe Threat_Maria_Carvalho_etal_INI_2022.pdfThe 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| dc.title.en_US.fl_str_mv |
The Threat Called Candida haemulonii Species Complex in Rio de Janeiro State, Brazil: Focus on Antifungal Resistance and Virulence Attributes |
| title |
The Threat Called Candida haemulonii Species Complex in Rio de Janeiro State, Brazil: Focus on Antifungal Resistance and Virulence Attributes |
| spellingShingle |
The Threat Called Candida haemulonii Species Complex in Rio de Janeiro State, Brazil: Focus on Antifungal Resistance and Virulence Attributes Ramos, Lívia S. Candida haemulonii complex Antifungal resistance Biofilm formation Hemolysins Hydrolytic enzymes Siderophores |
| title_short |
The Threat Called Candida haemulonii Species Complex in Rio de Janeiro State, Brazil: Focus on Antifungal Resistance and Virulence Attributes |
| title_full |
The Threat Called Candida haemulonii Species Complex in Rio de Janeiro State, Brazil: Focus on Antifungal Resistance and Virulence Attributes |
| title_fullStr |
The Threat Called Candida haemulonii Species Complex in Rio de Janeiro State, Brazil: Focus on Antifungal Resistance and Virulence Attributes |
| title_full_unstemmed |
The Threat Called Candida haemulonii Species Complex in Rio de Janeiro State, Brazil: Focus on Antifungal Resistance and Virulence Attributes |
| title_sort |
The Threat Called Candida haemulonii Species Complex in Rio de Janeiro State, Brazil: Focus on Antifungal Resistance and Virulence Attributes |
| author |
Ramos, Lívia S. |
| author_facet |
Ramos, Lívia S. Figueiredo-Carvalho, Maria Helena G. Silva, Laura N. Siqueira, Nahyara L. M. Lima, Joice C. Oliveira, Samuel S. Almeida-Paes, Rodrigo Zancopé-Oliveira, Rosely Maria Azevedo, Fabio S. Ferreira, Adriana L. P. Branquinha, Marta H. Santos, André L. S. |
| author_role |
author |
| author2 |
Figueiredo-Carvalho, Maria Helena G. Silva, Laura N. Siqueira, Nahyara L. M. Lima, Joice C. Oliveira, Samuel S. Almeida-Paes, Rodrigo Zancopé-Oliveira, Rosely Maria Azevedo, Fabio S. Ferreira, Adriana L. P. Branquinha, Marta H. Santos, André L. S. |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Ramos, Lívia S. Figueiredo-Carvalho, Maria Helena G. Silva, Laura N. Siqueira, Nahyara L. M. Lima, Joice C. Oliveira, Samuel S. Almeida-Paes, Rodrigo Zancopé-Oliveira, Rosely Maria Azevedo, Fabio S. Ferreira, Adriana L. P. Branquinha, Marta H. Santos, André L. S. |
| dc.subject.en.en_US.fl_str_mv |
Candida haemulonii complex Antifungal resistance Biofilm formation Hemolysins Hydrolytic enzymes Siderophores |
| topic |
Candida haemulonii complex Antifungal resistance Biofilm formation Hemolysins Hydrolytic enzymes Siderophores |
| description |
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil. |
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2022 |
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2022-11-07T19:01:35Z |
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2022-11-07T19:01:35Z |
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2022 |
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RAMOS, Lívia S. et al. The Threat Called Candida haemulonii Species Complex in Rio de Janeiro State, Brazil: Focus on Antifungal Resistance and Virulence Attributes. Journal of fungi, v. 8, v. 6, p. 1-19, 2022 |
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https://www.arca.fiocruz.br/handle/icict/55519 |
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2309-608X |
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10.3390/jof8060574 |
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RAMOS, Lívia S. et al. The Threat Called Candida haemulonii Species Complex in Rio de Janeiro State, Brazil: Focus on Antifungal Resistance and Virulence Attributes. Journal of fungi, v. 8, v. 6, p. 1-19, 2022 2309-608X 10.3390/jof8060574 |
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