Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/9496 |
Resumo: | Universidade Federal de Minas Gerais. Instituto de Ciencias Biolo gicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil |
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Silva, Patricia Costa Lima daScholte, Larissa Lopes SilvaReis, Mariana PaulaChaves, Anderson VieiraOliveira, Pollyanna Lacerda deItabayana, Luiza BastaniSuhadolnik, Maria LuizaBarbosa, Francisco Antonio RodriguesSouza, Edmar Chartone deNascimento, Andrea Maria Amaral2015-02-13T13:08:05Z2015-02-13T13:08:05Z2014SILVA, Patricia Costa Lima da, et al. Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining. PLoS ONE, v. 9, n. 4, p. e95655, 2014.1553-7366https://www.arca.fiocruz.br/handle/icict/949610.1371/journal.pone.0095655engPublic Library of ScienceBacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mininginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciencias Biolo gicas. Departamento de Biologia Geral. Belo Horizonte, MG, BrazilFundaçao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Grupo de Genomica e Biologia Computacional. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, BrazilThe bacterial community and genes involved in geobiocycling of arsenic (As) from sediment impacted by long-term gold mining were characterized through culture-based analysis of As-transforming bacteria and metagenomic studies of the arsC, arrA, and aioA genes. Sediment was collected from the historically gold mining impacted Mina stream, located in one of the world’s largest mining regions known as the “Iron Quadrangle”. A total of 123 As-resistant bacteria were recovered from the enrichment cultures, which were phenotypically and genotypically characterized for As-transformation. A diverse As-resistant bacteria community was found through phylogenetic analyses of the 16S rRNA gene. Bacterial isolates were affiliated with Proteobacteria, Firmicutes, and Actinobacteria and were represented by 20 genera. Most were AsV-reducing (72%), whereas AsIII-oxidizing accounted for 20%. Bacteria harboring the arsC gene predominated (85%), followed by aioA (20%) and arrA (7%). Additionally, we identified two novel As-transforming genera, Thermomonas and Pannonibacter. Metagenomic analysis of arsC, aioA, and arrA sequences confirmed the presence of these genes, with arrA sequences being more closely related to uncultured organisms. Evolutionary analyses revealed high genetic similarity between some arsC and aioA sequences obtained from isolates and clone libraries, suggesting that those isolates may represent environmentally important bacteria acting in As speciation. In addition, our findings show that the diversity ofarrA genes is wider than earlier described, once none arrA-OTUs were affiliated with known reference strains. Therefore, the molecular diversity of arrA genes is far from being fully explored deserving further attention.DNA sequence analysisSequence analysisPhylogeneticsSequence databasesPhylogenetic analysisRibosomal RNASequence alignmentinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9496/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINAL2014_077.pdf2014_077.pdfapplication/pdf2257835https://www.arca.fiocruz.br/bitstream/icict/9496/2/2014_077.pdf16792c762b812f79631d0aba9f07e577MD52TEXT2014_077.pdf.txt2014_077.pdf.txtExtracted texttext/plain54124https://www.arca.fiocruz.br/bitstream/icict/9496/3/2014_077.pdf.txt2cf6ab4ef38e99b76793b1bd3bbfa79eMD53icict/94962019-06-19 10:12:08.308oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:12:08Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining |
| title |
Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining |
| spellingShingle |
Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining Silva, Patricia Costa Lima da DNA sequence analysis Sequence analysis Phylogenetics Sequence databases Phylogenetic analysis Ribosomal RNA Sequence alignment |
| title_short |
Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining |
| title_full |
Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining |
| title_fullStr |
Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining |
| title_full_unstemmed |
Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining |
| title_sort |
Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining |
| author |
Silva, Patricia Costa Lima da |
| author_facet |
Silva, Patricia Costa Lima da Scholte, Larissa Lopes Silva Reis, Mariana Paula Chaves, Anderson Vieira Oliveira, Pollyanna Lacerda de Itabayana, Luiza Bastani Suhadolnik, Maria Luiza Barbosa, Francisco Antonio Rodrigues Souza, Edmar Chartone de Nascimento, Andrea Maria Amaral |
| author_role |
author |
| author2 |
Scholte, Larissa Lopes Silva Reis, Mariana Paula Chaves, Anderson Vieira Oliveira, Pollyanna Lacerda de Itabayana, Luiza Bastani Suhadolnik, Maria Luiza Barbosa, Francisco Antonio Rodrigues Souza, Edmar Chartone de Nascimento, Andrea Maria Amaral |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Patricia Costa Lima da Scholte, Larissa Lopes Silva Reis, Mariana Paula Chaves, Anderson Vieira Oliveira, Pollyanna Lacerda de Itabayana, Luiza Bastani Suhadolnik, Maria Luiza Barbosa, Francisco Antonio Rodrigues Souza, Edmar Chartone de Nascimento, Andrea Maria Amaral |
| dc.subject.en.pt_BR.fl_str_mv |
DNA sequence analysis Sequence analysis Phylogenetics Sequence databases Phylogenetic analysis Ribosomal RNA Sequence alignment |
| topic |
DNA sequence analysis Sequence analysis Phylogenetics Sequence databases Phylogenetic analysis Ribosomal RNA Sequence alignment |
| description |
Universidade Federal de Minas Gerais. Instituto de Ciencias Biolo gicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil |
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2014 |
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2014 |
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2015-02-13T13:08:05Z |
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2015-02-13T13:08:05Z |
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SILVA, Patricia Costa Lima da, et al. Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining. PLoS ONE, v. 9, n. 4, p. e95655, 2014. |
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1553-7366 |
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10.1371/journal.pone.0095655 |
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SILVA, Patricia Costa Lima da, et al. Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining. PLoS ONE, v. 9, n. 4, p. e95655, 2014. 1553-7366 10.1371/journal.pone.0095655 |
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