Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/58578 |
Resumo: | Fundação Altino Ventura. Department of Ophthalmology. Recife, PE, Brazil. |
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Costa, Camila de MoraesEspósito, Kayo PinheiroFlorêncio, Victor Medeiros BrandãoAquino, Paulo Romero LeiteFirmo, Elza FerreiraCosta, Iggor Macêdo do AmaralRocha, CamillaAndrade, Camila Galvão deOliveira-Júnior, Jorge BelémVentura, Camila V2023-05-23T13:39:55Z2023-05-23T13:39:55Z2021COSTA, C. DE M. et al. Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center. Arquivos Brasileiros de Oftalmologia, v. 84, n. 5, p. 449–453, set. 20211678-2925https://www.arca.fiocruz.br/handle/icict/5857810.5935/0004-2749.202100701678-2925porDrug Contamination*FluoresceinFungi*Gram-Positive BacteriaHumansOphthalmic SolutionsEvaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye centerinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Altino Ventura. Department of Ophthalmology. Recife, PE, Brazil.Universidade Federal de Pernambuco. Department of Tropical Medicine. Laboratory of Molecular Biology and Bacterial Genetics. Recife, PE, Brazil.JEA Laboratório de Análises Clínicas, Timbaúba, PE, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Department of Parasitology. Laboratory of Molecular and Celular Biology. Recife, PE, Brasil.Hospital de Olhos de Pernambuco. Department of Ophthalmology. Recife, PE, Brazil.Purpose: To analyze the presence of microorganisms in fluorescein eyedrops used in a reference eye center in Recife-PE. Methods: This real-life and masked study evaluated fluorescein eyedrops used at the Altino Ventura Foundation in May 2019. Cultures were performed according to exposure times; I) three eyedrop bottles were analyzed after one day of use, II) three eyedrop bottles after 4 d of use, III) three eyedrop bottles after 8 d of use, and IV) three unopened bottles used as control. Samples were collected from the bottle's tip, instilled drop, and residual fluid. After incubation, all colonies were analyzed and identified through biochemical tests. Results: The contamination rate of the fluorescein eyedrop bottles in this study was 55.5% (5/9 vials). There was no contamination in the control group. The highest contamination was seen in one day exposed eyedrops, in 100% of the bottles. The bottle's tip had a higher rate of contamination compared to the drop and residual fluid. Gram-positive bacteria were isolated in 7/27 (25.9%) samples. Growth of fungi or gram-negative bacteria was not observed. Conclusion: The identification of gram-positive bacteria predominantly on the tip of the fluorescein eyedrop bottles suggests inadequate handling as the main cause of contamination.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALEvaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center.pdfEvaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center.pdfapplication/pdf439909https://www.arca.fiocruz.br/bitstream/icict/58578/2/Evaluation%20of%20microbial%20contamination%20in%20multi-dose%20fluorescein%20eyedrops%20in%20a%20reference%20eye%20center.pdfd2ade0e8329c852760d4ca481c293564MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/58578/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/585782023-05-23 10:39:56.048oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-05-23T13:39:56Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
| title |
Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
| spellingShingle |
Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center Costa, Camila de Moraes Drug Contamination* Fluorescein Fungi* Gram-Positive Bacteria Humans Ophthalmic Solutions |
| title_short |
Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
| title_full |
Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
| title_fullStr |
Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
| title_full_unstemmed |
Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
| title_sort |
Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
| author |
Costa, Camila de Moraes |
| author_facet |
Costa, Camila de Moraes Espósito, Kayo Pinheiro Florêncio, Victor Medeiros Brandão Aquino, Paulo Romero Leite Firmo, Elza Ferreira Costa, Iggor Macêdo do Amaral Rocha, Camilla Andrade, Camila Galvão de Oliveira-Júnior, Jorge Belém Ventura, Camila V |
| author_role |
author |
| author2 |
Espósito, Kayo Pinheiro Florêncio, Victor Medeiros Brandão Aquino, Paulo Romero Leite Firmo, Elza Ferreira Costa, Iggor Macêdo do Amaral Rocha, Camilla Andrade, Camila Galvão de Oliveira-Júnior, Jorge Belém Ventura, Camila V |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Costa, Camila de Moraes Espósito, Kayo Pinheiro Florêncio, Victor Medeiros Brandão Aquino, Paulo Romero Leite Firmo, Elza Ferreira Costa, Iggor Macêdo do Amaral Rocha, Camilla Andrade, Camila Galvão de Oliveira-Júnior, Jorge Belém Ventura, Camila V |
| dc.subject.mesh.en_US.fl_str_mv |
Drug Contamination* Fluorescein Fungi* Gram-Positive Bacteria Humans Ophthalmic Solutions |
| topic |
Drug Contamination* Fluorescein Fungi* Gram-Positive Bacteria Humans Ophthalmic Solutions |
| description |
Fundação Altino Ventura. Department of Ophthalmology. Recife, PE, Brazil. |
| publishDate |
2021 |
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2021 |
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2023-05-23T13:39:55Z |
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2023-05-23T13:39:55Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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COSTA, C. DE M. et al. Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center. Arquivos Brasileiros de Oftalmologia, v. 84, n. 5, p. 449–453, set. 2021 |
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https://www.arca.fiocruz.br/handle/icict/58578 |
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1678-2925 |
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10.5935/0004-2749.20210070 |
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1678-2925 |
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COSTA, C. DE M. et al. Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center. Arquivos Brasileiros de Oftalmologia, v. 84, n. 5, p. 449–453, set. 2021 1678-2925 10.5935/0004-2749.20210070 |
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