Mycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functions
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/46626 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil. |
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Schwarz, Marcos Gustavo AraujoAntunes, DeborahCorrêa, Paloma RezendeGonçalves, Antonio José da SilvaMalaga, WladimirCaffarena, Ernesto RaulGuilhot, ChristopheLima, Leila Mendonça2021-04-11T13:35:12Z2021-04-11T13:35:12Z2021SCHWARZ, Marcos Gustavo Araujo et al. Mycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functions. Frontiers in Microbiology, v. 11, Article 624121, p. 1-15, Jan. 2021.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/4662610.3389/fmicb.2020.624121engFrontiers MediaMycobacterium bovis BCG MoreauFumarato redutaseSequência homopoliméricaDeslizamento transcricionalVacina contra TuberculoseFumarate reductaseMycobacterium bovis BCG MoreauHomopolymeric sequenceTranscriptional slippageTuberculosis vaccineMycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, Université Paul Sabatier. Toulouse, France.Fundação Oswaldo Cruz. Presidência. Programa de Computação Científica. Grupo de Biofísica Computacional e Modelagem Molécula. Rio de Janeiro, RJ, Brasil.Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, Université Paul Sabatier. Toulouse, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Tuberculosis is a world widespread disease, caused by Mycobacterium tuberculosis (M.tb). Although considered an obligate aerobe, this organism can resist life-limiting conditions such as microaerophily mainly due to its set of enzymes responsible for energy production and coenzyme restoration under these conditions. One of these enzymes is fumarate reductase, an heterotetrameric complex composed of a catalytic (FrdA), an iron-sulfur cluster (FrdB) and two transmembrane (FrdC and FrdD) subunits involved in anaerobic respiration and important for the maintenance of membrane potential. In this work, aiming to further characterize this enzyme function in mycobacteria, we analyzed the expression of FrdB-containing proteins in M.tb and Mycobacterium bovis Bacillus Calmette–Guérin (BCG) Moreau, the Brazilian vaccine strain against tuberculosis. We identified three isoforms in both mycobacteria, two of them corresponding to the predicted encoded polypeptides of M.tb (27 kDa) and BCG Moreau (40 kDa) frd sequences, as due to an insertion on the latter’s operon a fused FrdBC protein is expected. The third 52 kDa band can be explained by a transcriptional slippage event, typically occurring when mutation arises in a repetitive region within a coding sequence, thought to reduce its impact allowing the production of both native and variant forms. Comparative modeling of the M.tb and BCG Moreau predicted protein complexes allowed the detection of subtle overall differences, showing a high degree of structure and maybe functional resemblance among them. Axenic growth and macrophage infection assays show that the frd locus is important for proper bacterial development in both scenarios, and that both M.tb’s and BCG Moreau’s alleles can partially revert the hampered phenotype of the knockout strain. Altogether, our results show that the frdABCD operon of Mycobacteria may have evolved to possess other yet non-described functions, such as those necessary during aerobic logarithmic growth and early stage steps of infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/46626/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDeboraAntunes_AntonioSGonçalves_etal_IOC_2021.pdfDeboraAntunes_AntonioSGonçalves_etal_IOC_2021.pdfapplication/pdf5565628https://www.arca.fiocruz.br/bitstream/icict/46626/2/DeboraAntunes_AntonioSGon%c3%a7alves_etal_IOC_2021.pdf5431b6f3c4ff4861ef8b270c26c8754fMD52TEXTDeboraAntunes_AntonioSGonçalves_etal_IOC_2021.pdf.txtDeboraAntunes_AntonioSGonçalves_etal_IOC_2021.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Mycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functions |
| title |
Mycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functions |
| spellingShingle |
Mycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functions Schwarz, Marcos Gustavo Araujo Mycobacterium bovis BCG Moreau Fumarato redutase Sequência homopolimérica Deslizamento transcricional Vacina contra Tuberculose Fumarate reductase Mycobacterium bovis BCG Moreau Homopolymeric sequence Transcriptional slippage Tuberculosis vaccine |
| title_short |
Mycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functions |
| title_full |
Mycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functions |
| title_fullStr |
Mycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functions |
| title_full_unstemmed |
Mycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functions |
| title_sort |
Mycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functions |
| author |
Schwarz, Marcos Gustavo Araujo |
| author_facet |
Schwarz, Marcos Gustavo Araujo Antunes, Deborah Corrêa, Paloma Rezende Gonçalves, Antonio José da Silva Malaga, Wladimir Caffarena, Ernesto Raul Guilhot, Christophe Lima, Leila Mendonça |
| author_role |
author |
| author2 |
Antunes, Deborah Corrêa, Paloma Rezende Gonçalves, Antonio José da Silva Malaga, Wladimir Caffarena, Ernesto Raul Guilhot, Christophe Lima, Leila Mendonça |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Schwarz, Marcos Gustavo Araujo Antunes, Deborah Corrêa, Paloma Rezende Gonçalves, Antonio José da Silva Malaga, Wladimir Caffarena, Ernesto Raul Guilhot, Christophe Lima, Leila Mendonça |
| dc.subject.other.pt_BR.fl_str_mv |
Mycobacterium bovis BCG Moreau Fumarato redutase Sequência homopolimérica Deslizamento transcricional Vacina contra Tuberculose |
| topic |
Mycobacterium bovis BCG Moreau Fumarato redutase Sequência homopolimérica Deslizamento transcricional Vacina contra Tuberculose Fumarate reductase Mycobacterium bovis BCG Moreau Homopolymeric sequence Transcriptional slippage Tuberculosis vaccine |
| dc.subject.en.pt_BR.fl_str_mv |
Fumarate reductase Mycobacterium bovis BCG Moreau Homopolymeric sequence Transcriptional slippage Tuberculosis vaccine |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil. |
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2021 |
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2021-04-11T13:35:12Z |
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2021-04-11T13:35:12Z |
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2021 |
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SCHWARZ, Marcos Gustavo Araujo et al. Mycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functions. Frontiers in Microbiology, v. 11, Article 624121, p. 1-15, Jan. 2021. |
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https://www.arca.fiocruz.br/handle/icict/46626 |
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1664-302X |
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10.3389/fmicb.2020.624121 |
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SCHWARZ, Marcos Gustavo Araujo et al. Mycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functions. Frontiers in Microbiology, v. 11, Article 624121, p. 1-15, Jan. 2021. 1664-302X 10.3389/fmicb.2020.624121 |
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