Application of Direct Agglutination Test (DAT) and Fast Agglutination Screening Test (FAST) for sero-diagnosis of visceral leishmaniasis in endemic area of Minas Gerais, Brazil

Detalhes bibliográficos
Autor(a) principal: Silva, Eduardo S.
Data de Publicação: 2005
Outros Autores: Schoone, Gerard J., Gontijo, Célia Maria Ferreira, Brazil, Reginaldo Peçanha, Pacheco, Raquel S., Schallig, Henk D. F. H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/6779
Resumo: Universidade do Estado de Minas Gerais. Belo Horizonte, MG, Brasil / Fundação Educacional de Divinópolis. Divinópolis, MG, Brasil.
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spelling Silva, Eduardo S.Schoone, Gerard J.Gontijo, Célia Maria FerreiraBrazil, Reginaldo PeçanhaPacheco, Raquel S.Schallig, Henk D. F. H.2013-08-09T17:22:38Z2013-08-09T17:22:38Z2005SILVA, Eduardo S et al. Application of Direct Agglutination Test (DAT) and Fast Agglutination Screening Test (FAST) for sero-diagnosis of visceral leishmaniasis in endemic area of Minas Gerais, Brazil. Kinetoplastid Biology and Disease, v. 4, n. 4, p. 1-5, 2005.1475-9292https://www.arca.fiocruz.br/handle/icict/6779doi:10.1186/1475-9292-4-4engApplication of Direct Agglutination Test (DAT) and Fast Agglutination Screening Test (FAST) for sero-diagnosis of visceral leishmaniasis in endemic area of Minas Gerais, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado de Minas Gerais. Belo Horizonte, MG, Brasil / Fundação Educacional de Divinópolis. Divinópolis, MG, Brasil.Koninklijk Instituut voor de Tropen / Royal Tropical Institute. Biomedical Research. Amsterdam, The Netherlands.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Koninklijk Instituut voor de Tropen / Royal Tropical Institute. Biomedical Research. Amsterdam, The Netherlands.Background: The direct agglutination test (DAT) has proved to be a very important sero-diagnostic tool combining high levels of intrinsic validity and ease of performance. Otherwise, fast agglutination screening test (FAST) utilises only one serum dilution making the test very suitable for the screening of large populations. Results: We have tested FAST and DAT for the detection anti-Leishmania antibodies in serum samples from patients with American visceral (AVL) and cutaneous leishmaniases (ACL) in Minas Gerais State, Brazil. The DAT on serum and blood samples of confirmed AVL patients found all samples positive at a serum dilution of ≥1:800. This dilution was subsequently used as cut off value in the study. The blood and serum samples of these confirmed patients could also be clearly read in FAST using a 1:100 dilution with the same high sensitivity. DAT and FAST were not able to detect significant amounts of antibodies in samples from ACL patients and are not suitable for the diagnosis of this manifestation of the disease. Conclusion: We suggest that both DAT and FAST are very practical diagnostic tools for the sero-diagnosis of AVL under rural conditions as bothserological tests do not require sophisticated equipment, a cold chain and are very simple to perform.Sero-diagnosisElisaPCRDirect Agglutination TestFast Agglutination Screening Testinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINAL79. Application of Direct Agglutination Test (DAT) and Fast.pdf79. Application of Direct Agglutination Test (DAT) and Fast.pdfapplication/pdf230764https://www.arca.fiocruz.br/bitstream/icict/6779/1/79.%20Application%20of%20Direct%20Agglutination%20Test%20%28DAT%29%20and%20Fast.pdfae2471a279610cf0d6661910619dd8c8MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/6779/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXT79. 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dc.title.pt_BR.fl_str_mv Application of Direct Agglutination Test (DAT) and Fast Agglutination Screening Test (FAST) for sero-diagnosis of visceral leishmaniasis in endemic area of Minas Gerais, Brazil
title Application of Direct Agglutination Test (DAT) and Fast Agglutination Screening Test (FAST) for sero-diagnosis of visceral leishmaniasis in endemic area of Minas Gerais, Brazil
spellingShingle Application of Direct Agglutination Test (DAT) and Fast Agglutination Screening Test (FAST) for sero-diagnosis of visceral leishmaniasis in endemic area of Minas Gerais, Brazil
Silva, Eduardo S.
Sero-diagnosis
Elisa
PCR
Direct Agglutination Test
Fast Agglutination Screening Test
title_short Application of Direct Agglutination Test (DAT) and Fast Agglutination Screening Test (FAST) for sero-diagnosis of visceral leishmaniasis in endemic area of Minas Gerais, Brazil
title_full Application of Direct Agglutination Test (DAT) and Fast Agglutination Screening Test (FAST) for sero-diagnosis of visceral leishmaniasis in endemic area of Minas Gerais, Brazil
title_fullStr Application of Direct Agglutination Test (DAT) and Fast Agglutination Screening Test (FAST) for sero-diagnosis of visceral leishmaniasis in endemic area of Minas Gerais, Brazil
title_full_unstemmed Application of Direct Agglutination Test (DAT) and Fast Agglutination Screening Test (FAST) for sero-diagnosis of visceral leishmaniasis in endemic area of Minas Gerais, Brazil
title_sort Application of Direct Agglutination Test (DAT) and Fast Agglutination Screening Test (FAST) for sero-diagnosis of visceral leishmaniasis in endemic area of Minas Gerais, Brazil
author Silva, Eduardo S.
author_facet Silva, Eduardo S.
Schoone, Gerard J.
Gontijo, Célia Maria Ferreira
Brazil, Reginaldo Peçanha
Pacheco, Raquel S.
Schallig, Henk D. F. H.
author_role author
author2 Schoone, Gerard J.
Gontijo, Célia Maria Ferreira
Brazil, Reginaldo Peçanha
Pacheco, Raquel S.
Schallig, Henk D. F. H.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Eduardo S.
Schoone, Gerard J.
Gontijo, Célia Maria Ferreira
Brazil, Reginaldo Peçanha
Pacheco, Raquel S.
Schallig, Henk D. F. H.
dc.subject.en.pt_BR.fl_str_mv Sero-diagnosis
Elisa
PCR
Direct Agglutination Test
Fast Agglutination Screening Test
topic Sero-diagnosis
Elisa
PCR
Direct Agglutination Test
Fast Agglutination Screening Test
description Universidade do Estado de Minas Gerais. Belo Horizonte, MG, Brasil / Fundação Educacional de Divinópolis. Divinópolis, MG, Brasil.
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2013-08-09T17:22:38Z
dc.date.available.fl_str_mv 2013-08-09T17:22:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Eduardo S et al. Application of Direct Agglutination Test (DAT) and Fast Agglutination Screening Test (FAST) for sero-diagnosis of visceral leishmaniasis in endemic area of Minas Gerais, Brazil. Kinetoplastid Biology and Disease, v. 4, n. 4, p. 1-5, 2005.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/6779
dc.identifier.issn.none.fl_str_mv 1475-9292
dc.identifier.doi.none.fl_str_mv doi:10.1186/1475-9292-4-4
identifier_str_mv SILVA, Eduardo S et al. Application of Direct Agglutination Test (DAT) and Fast Agglutination Screening Test (FAST) for sero-diagnosis of visceral leishmaniasis in endemic area of Minas Gerais, Brazil. Kinetoplastid Biology and Disease, v. 4, n. 4, p. 1-5, 2005.
1475-9292
doi:10.1186/1475-9292-4-4
url https://www.arca.fiocruz.br/handle/icict/6779
dc.language.iso.fl_str_mv eng
language eng
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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