The calcium transporter Pmc1 provides Ca2+ tolerance and influences the progression of murine cryptococcal infection

Detalhes bibliográficos
Autor(a) principal: Kmetzsch, Lívia
Data de Publicação: 2013
Outros Autores: Staats, Charley C., Cupertino, Julia B., Fonseca, Fernanda Lopes, Rodrigues, Marcio Lourenço, Schrank, Augusto, Vainstein, Marilene Henning
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31992
Resumo: Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brasil.
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spelling Kmetzsch, LíviaStaats, Charley C.Cupertino, Julia B.Fonseca, Fernanda LopesRodrigues, Marcio LourençoSchrank, AugustoVainstein, Marilene Henning2019-03-07T20:00:12Z2019-03-07T20:00:12Z2013KMETZSCH, Livia et al. The calcium transporter Pmc1 provides Ca2+ tolerance and influences the progression of murine cryptococcal infection. FEBS Journal, v. 280, p. 4853–4864, 2013.1742-464Xhttps://www.arca.fiocruz.br/handle/icict/3199210.1111/febs.12458engFEBSThe calcium transporter Pmc1 provides Ca2+ tolerance and influences the progression of murine cryptococcal infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de G oes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de G oes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brasil.The Ca(2+)-calcineurin signaling pathway in the human fungal pathogen Cryptococcus neoformans is essential for adaptation to the host environment during infection. Calcium transporters regulate cytosolic calcium concentrations, providing Ca(2+) loading into storage organelles. The three calcium transporters that have been characterized in C. neoformans, Cch1, Eca1 and Vcx1, are required for fungal virulence, supporting a role for calcium-mediated signaling in cryptococcal pathogenesis. In the present study, we report the functional characterization of the putative vacuolar calcium ATPase Pmc1 in C. neoformans. Our results demonstrate that Pmc1 provides tolerance to high Ca(2+) concentrations. The double knockout of C. neoformans PMC1 and VCX1 genes impaired the intracellular calcium transport, resulting in a significant increase in cytosolic calcium levels. Furthermore, Pmc1 was essential for both the progression of pulmonary infection and brain colonization in mice, emphasizing the crucial role of calcium signaling and transport for cryptococcal pathogenesis.Calcium transportCryptococcosisCryptococcus neoformansVacuolar calcium storageVirulenceinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83101https://www.arca.fiocruz.br/bitstream/icict/31992/1/license.txt425ef259bcb460998407fbfed7c102d0MD51ORIGINALve_Marcio_Rodrigues_etal_CDTS_2013c.pdfve_Marcio_Rodrigues_etal_CDTS_2013c.pdfapplication/pdf654095https://www.arca.fiocruz.br/bitstream/icict/31992/2/ve_Marcio_Rodrigues_etal_CDTS_2013c.pdf2c4916d4e295d8e8485b645fd88d7f58MD52TEXTve_Marcio_Rodrigues_etal_CDTS_2013c.pdf.txtve_Marcio_Rodrigues_etal_CDTS_2013c.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv The calcium transporter Pmc1 provides Ca2+ tolerance and influences the progression of murine cryptococcal infection
title The calcium transporter Pmc1 provides Ca2+ tolerance and influences the progression of murine cryptococcal infection
spellingShingle The calcium transporter Pmc1 provides Ca2+ tolerance and influences the progression of murine cryptococcal infection
Kmetzsch, Lívia
Calcium transport
Cryptococcosis
Cryptococcus neoformans
Vacuolar calcium storage
Virulence
title_short The calcium transporter Pmc1 provides Ca2+ tolerance and influences the progression of murine cryptococcal infection
title_full The calcium transporter Pmc1 provides Ca2+ tolerance and influences the progression of murine cryptococcal infection
title_fullStr The calcium transporter Pmc1 provides Ca2+ tolerance and influences the progression of murine cryptococcal infection
title_full_unstemmed The calcium transporter Pmc1 provides Ca2+ tolerance and influences the progression of murine cryptococcal infection
title_sort The calcium transporter Pmc1 provides Ca2+ tolerance and influences the progression of murine cryptococcal infection
author Kmetzsch, Lívia
author_facet Kmetzsch, Lívia
Staats, Charley C.
Cupertino, Julia B.
Fonseca, Fernanda Lopes
Rodrigues, Marcio Lourenço
Schrank, Augusto
Vainstein, Marilene Henning
author_role author
author2 Staats, Charley C.
Cupertino, Julia B.
Fonseca, Fernanda Lopes
Rodrigues, Marcio Lourenço
Schrank, Augusto
Vainstein, Marilene Henning
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Kmetzsch, Lívia
Staats, Charley C.
Cupertino, Julia B.
Fonseca, Fernanda Lopes
Rodrigues, Marcio Lourenço
Schrank, Augusto
Vainstein, Marilene Henning
dc.subject.en.pt_BR.fl_str_mv Calcium transport
Cryptococcosis
Cryptococcus neoformans
Vacuolar calcium storage
Virulence
topic Calcium transport
Cryptococcosis
Cryptococcus neoformans
Vacuolar calcium storage
Virulence
description Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2019-03-07T20:00:12Z
dc.date.available.fl_str_mv 2019-03-07T20:00:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv KMETZSCH, Livia et al. The calcium transporter Pmc1 provides Ca2+ tolerance and influences the progression of murine cryptococcal infection. FEBS Journal, v. 280, p. 4853–4864, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31992
dc.identifier.issn.pt_BR.fl_str_mv 1742-464X
dc.identifier.doi.none.fl_str_mv 10.1111/febs.12458
identifier_str_mv KMETZSCH, Livia et al. The calcium transporter Pmc1 provides Ca2+ tolerance and influences the progression of murine cryptococcal infection. FEBS Journal, v. 280, p. 4853–4864, 2013.
1742-464X
10.1111/febs.12458
url https://www.arca.fiocruz.br/handle/icict/31992
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv FEBS
publisher.none.fl_str_mv FEBS
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/31992/2/ve_Marcio_Rodrigues_etal_CDTS_2013c.pdf
https://www.arca.fiocruz.br/bitstream/icict/31992/3/ve_Marcio_Rodrigues_etal_CDTS_2013c.pdf.txt
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