Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection

Detalhes bibliográficos
Autor(a) principal: Fonseca, Denise Morais da
Data de Publicação: 2007
Outros Autores: Silva, Célio Lopes, Paula, Marina Oliveira e, Soares, Edson Garcia, Marchal, Gilles, Horn, Cynthia, Bonato, Vânia Luiza Deperon
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/57312
Resumo: We would like to thank Izaı´ra Tincani Branda˜o and Ana Paula Masson for the general technical assistance provided, as well as Ana Maria da Rocha for her technical assistance in processing the histological sections. This study received financial support from the Fundac¸ão de Amparo a Pesquisa do Estado de São Paulo (FAPESP, Foundation for the Support of Research in the State of Sa˜o Paulo; grant no. 03/11303-2 and 00/09663-2), the Conselho Nacional de Desenvolvimento Cientı´fico e Tecnolo´ gico (CNPq, Council for Scientific and Technological Development), and the Rede Brasileira de Pesquisa em TB (Rede-TB, Brazilian Tuberculosis Research Network).
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spelling Fonseca, Denise Morais daSilva, Célio LopesPaula, Marina Oliveira eSoares, Edson GarciaMarchal, GillesHorn, CynthiaBonato, Vânia Luiza Deperon2023-03-09T02:44:00Z2023-03-09T02:44:00Z2007FONSECA, Denise Morais da et al. Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection. Immunology, Oxford, v. 121, n. 4, p. 508-517, Aug. 2007.1365-2567https://www.arca.fiocruz.br/handle/icict/5731210.1111/j.1365-2567.2007.02597.x1365-2567We would like to thank Izaı´ra Tincani Branda˜o and Ana Paula Masson for the general technical assistance provided, as well as Ana Maria da Rocha for her technical assistance in processing the histological sections. This study received financial support from the Fundac¸ão de Amparo a Pesquisa do Estado de São Paulo (FAPESP, Foundation for the Support of Research in the State of Sa˜o Paulo; grant no. 03/11303-2 and 00/09663-2), the Conselho Nacional de Desenvolvimento Cientı´fico e Tecnolo´ gico (CNPq, Council for Scientific and Technological Development), and the Rede Brasileira de Pesquisa em TB (Rede-TB, Brazilian Tuberculosis Research Network).University of São Paulo. Ribeirão Preto School of Medicine. Department of Biochemistry and Immunology. Tuberculosis Research Centre. Ribeirão Preto, SP, Brazil.University of São Paulo. Ribeirão Preto School of Medicine. Department of Biochemistry and Immunology. Tuberculosis Research Centre. Ribeirão Preto, SP, Brazil.University of São Paulo. Ribeirão Preto School of Medicine. Department of Biochemistry and Immunology. Tuberculosis Research Centre. Ribeirão Preto, SP, Brazil.University of São Paulo. Ribeirão Preto School of Medicine. Department of Pathology. Ribeirão Preto, SP, Brazil.Institute Pasteur. Laboratoire de Référence des Mycobacteries. Paris, France.Oswaldo Cruz Foundation. Evandro Chagas Clinical Research Institute. Rio de Janeiro, RJ, Brazil.University of São Paulo. Ribeirão Preto School of Medicine. Department of Biochemistry and Immunology. Tuberculosis Research Centre. Ribeirão Preto, SP, Brazil.The results of various animal model studies of tuberculosis (TB) suggest that culture filtrate proteins (CFPs), which are antigens secreted by Mycobacterium tuberculosis, are largely responsible for improvements in TB vaccines. The great obstacle to developing protein subunit vaccines is that adjuvants are required in order to stimulate relevant protective immune responses. Acting as immune adjuvants, CpG-oligodeoxynucleotides (CpG-ODNs) promote the activation of Th1 cells and of pro-inflammatory cytokines. To evaluate the adjuvant role of CpG-ODNs in conferring enhanced immunogenic capacity and protection against M. tuberculosis, we immunized mice with CFP antigen combined with synthetic CpG-ODNs (CFP/CpG) or with incomplete Freund's adjuvant (IFA) (CFP/IFA). Immunization with CFP/CpG induced a T helper 1 (Th1)-biased response accompanied by a higher immunoglobulin G2a (IgG2a) antibody/IgG1 antibody ratio, elevated production of interferon-gamma (IFN-gamma) by spleen cells and in lungs. However, CFP/IFA-immunized mice presented higher levels of IgG1 antibodies, as well as increased production of IFN-gamma, interleukin (IL)-5, and IL-10 by spleen cells, together with lower levels of IFN-gamma in the lungs. Despite the stronger Th1 response seen in both groups, believed to be necessary for protection against TB, only mice immunized with CFP/IFA were protected after M. tuberculosis infection. Lung histology revealed that lung parenchyma were better preserved in CFP/IFA-immunized mice, which also presented intense lymphocyte recruitment to the lesion, whereas CFP/CpG-immunized mice presented severe pulmonary injury accompanied by necrosis. Based on the data presented, we discuss the widely accepted paradigm that high levels of IFN-gamma are directly correlated with protection against experimental TB.engWiley Online LibraryIncreased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTuberculosisCulture filtrate proteinsCpG oligodeoxynucleotidesVaccineAdjuvantinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALve_Fonseca_Denise_etal_INI_2007.pdfve_Fonseca_Denise_etal_INI_2007.pdfapplication/pdf604897https://www.arca.fiocruz.br/bitstream/icict/57312/2/ve_Fonseca_Denise_etal_INI_2007.pdfd1b636a5d4010982cf49fab73ed2a6c3MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/57312/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/573122023-03-08 23:47:49.796oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-09T02:47:49Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection
title Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection
spellingShingle Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection
Fonseca, Denise Morais da
Tuberculosis
Culture filtrate proteins
CpG oligodeoxynucleotides
Vaccine
Adjuvant
title_short Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection
title_full Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection
title_fullStr Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection
title_full_unstemmed Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection
title_sort Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection
author Fonseca, Denise Morais da
author_facet Fonseca, Denise Morais da
Silva, Célio Lopes
Paula, Marina Oliveira e
Soares, Edson Garcia
Marchal, Gilles
Horn, Cynthia
Bonato, Vânia Luiza Deperon
author_role author
author2 Silva, Célio Lopes
Paula, Marina Oliveira e
Soares, Edson Garcia
Marchal, Gilles
Horn, Cynthia
Bonato, Vânia Luiza Deperon
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fonseca, Denise Morais da
Silva, Célio Lopes
Paula, Marina Oliveira e
Soares, Edson Garcia
Marchal, Gilles
Horn, Cynthia
Bonato, Vânia Luiza Deperon
dc.subject.en.en_US.fl_str_mv Tuberculosis
Culture filtrate proteins
CpG oligodeoxynucleotides
Vaccine
Adjuvant
topic Tuberculosis
Culture filtrate proteins
CpG oligodeoxynucleotides
Vaccine
Adjuvant
description We would like to thank Izaı´ra Tincani Branda˜o and Ana Paula Masson for the general technical assistance provided, as well as Ana Maria da Rocha for her technical assistance in processing the histological sections. This study received financial support from the Fundac¸ão de Amparo a Pesquisa do Estado de São Paulo (FAPESP, Foundation for the Support of Research in the State of Sa˜o Paulo; grant no. 03/11303-2 and 00/09663-2), the Conselho Nacional de Desenvolvimento Cientı´fico e Tecnolo´ gico (CNPq, Council for Scientific and Technological Development), and the Rede Brasileira de Pesquisa em TB (Rede-TB, Brazilian Tuberculosis Research Network).
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2023-03-09T02:44:00Z
dc.date.available.fl_str_mv 2023-03-09T02:44:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv FONSECA, Denise Morais da et al. Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection. Immunology, Oxford, v. 121, n. 4, p. 508-517, Aug. 2007.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/57312
dc.identifier.issn.en_US.fl_str_mv 1365-2567
dc.identifier.doi.none.fl_str_mv 10.1111/j.1365-2567.2007.02597.x
dc.identifier.eissn.none.fl_str_mv 1365-2567
identifier_str_mv FONSECA, Denise Morais da et al. Increased levels of interferon-c primed by culture filtrate proteins antigen and CpG-ODN immunization do not confer significant protection against Mycobacterium tuberculosis infection. Immunology, Oxford, v. 121, n. 4, p. 508-517, Aug. 2007.
1365-2567
10.1111/j.1365-2567.2007.02597.x
url https://www.arca.fiocruz.br/handle/icict/57312
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Wiley Online Library
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