Novel Catalytically-Inactive PII Metalloproteinases from a Viperid Snake Venom with Substitutions in the Canonical Zinc-Binding Motif

Detalhes bibliográficos
Autor(a) principal: Camacho, Erika
Data de Publicação: 2016
Outros Autores: Sanz, Libia, Escalante, Teresa, Pérez, Alicia, Villalta, Fabián, Lomonte, Bruno, Ferreira, Ana Gisele C. Neves, Feoli, Andrés, Calvete, Juan J., Gutiérrez, José María, Rucavado, Alexandra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18016
Resumo: Universidad de Costa Rica. Instituto Clodomiro Picado. Facultad de Microbiologia. San Jose, Costa, Rica.
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spelling Camacho, ErikaSanz, LibiaEscalante, TeresaPérez, AliciaVillalta, FabiánLomonte, BrunoFerreira, Ana Gisele C. NevesFeoli, AndrésCalvete, Juan J.Gutiérrez, José MaríaRucavado, Alexandra2017-03-13T13:26:02Z2017-03-13T13:26:02Z2016CAMACHO, Erika; et al. Novel Catalytically-Inactive PII Metalloproteinases from a Viperid Snake Venom with Substitutions in the Canonical Zinc-Binding Motif. Toxins (Basel), v.8, n.10:292,18 p, 2016.2072-6651https://www.arca.fiocruz.br/handle/icict/1801610.3390/toxins81002922072-6651engMDPIMetaloproteinases de veneno de cobraDomínio da desintegraçãoMotivo de ligação ao zincoAtividade hemorrágicaAgregação de plaquetasAtividade de proteinasesnake venom metalloproteinasesPII SVMP homologuesdisintegrin domainzinc-binding motifhemorrhagic activityplatelet aggregationproteinase activityNovel Catalytically-Inactive PII Metalloproteinases from a Viperid Snake Venom with Substitutions in the Canonical Zinc-Binding Motifinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidad de Costa Rica. Instituto Clodomiro Picado. Facultad de Microbiologia. San Jose, Costa, Rica.Instituto de Biomedicina de Valencia. Consejo Superior de Investigaciones Científicas. Valencia, Spain.Universidad de Costa Rica. Instituto Clodomiro Picado. Facultad de Microbiologia. San Jose, Costa, Rica.Instituto de Biomedicina de Valencia. Consejo Superior de Investigaciones Científicas. Valencia, Spain.Universidad de Costa Rica. Instituto Clodomiro Picado. Facultad de Microbiologia. San Jose, Costa, Rica.Universidad de Costa Rica. Instituto Clodomiro Picado. Facultad de Microbiologia. San Jose, Costa, Rica.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil.Universidad de Costa Rica. Instituto Clodomiro Picado. Facultad de Microbiologia. San Jose, Costa, Rica.Instituto de Biomedicina de Valencia. Consejo Superior de Investigaciones Científicas. Valencia, Spain / Universidad Politécnica de Valencia. Departamento de Biotecnologia. Valencia, Spain.Universidad de Costa Rica. Instituto Clodomiro Picado. Facultad de Microbiologia. San Jose, Costa, Rica.Universidad de Costa Rica. Instituto Clodomiro Picado. Facultad de Microbiologia. San Jose, Costa, Rica.Snake venom metalloproteinases (SVMPs) play key biological roles in prey immobilization and digestion. The majority of these activities depend on the hydrolysis of relevant protein substrates in the tissues. Hereby, we describe several isoforms and a cDNA clone sequence, corresponding to PII SVMP homologues from the venom of the Central American pit viper Bothriechis lateralis, which have modifications in the residues of the canonical sequence of the zinc-binding motif HEXXHXXGXXH. As a consequence, the proteolytic activity of the isolated proteins was undetectable when tested on azocasein and gelatin. These PII isoforms comprise metalloproteinase and disintegrin domains in the mature protein, thus belonging to the subclass PIIb of SVMPs. PII SVMP homologues were devoid of hemorrhagic and in vitro coagulant activities, effects attributed to the enzymatic activity of SVMPs, but induced a mild edema. One of the isoforms presents the characteristic RGD sequence in the disintegrin domain and inhibits ADP- and collagen-induced platelet aggregation. Catalytically-inactive SVMP homologues may have been hitherto missed in the characterization of snake venoms. The presence of such enzymatically-inactive homologues in snake venoms and their possible toxic and adaptive roles deserve further investigation.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18016/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALanagisele_ferreira_etal_IOC_2016.pdfanagisele_ferreira_etal_IOC_2016.pdfapplication/pdf2698407https://www.arca.fiocruz.br/bitstream/icict/18016/2/anagisele_ferreira_etal_IOC_2016.pdf1e04d4ba442921a99eea2c9925849422MD52TEXTanagisele_ferreira_etal_IOC_2016.pdf.txtanagisele_ferreira_etal_IOC_2016.pdf.txtExtracted texttext/plain80086https://www.arca.fiocruz.br/bitstream/icict/18016/3/anagisele_ferreira_etal_IOC_2016.pdf.txt998ea3d2780fbe932a6f5c8e481085e4MD53icict/180162023-09-05 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dc.title.pt_BR.fl_str_mv Novel Catalytically-Inactive PII Metalloproteinases from a Viperid Snake Venom with Substitutions in the Canonical Zinc-Binding Motif
title Novel Catalytically-Inactive PII Metalloproteinases from a Viperid Snake Venom with Substitutions in the Canonical Zinc-Binding Motif
spellingShingle Novel Catalytically-Inactive PII Metalloproteinases from a Viperid Snake Venom with Substitutions in the Canonical Zinc-Binding Motif
Camacho, Erika
Metaloproteinases de veneno de cobra
Domínio da desintegração
Motivo de ligação ao zinco
Atividade hemorrágica
Agregação de plaquetas
Atividade de proteinase
snake venom metalloproteinases
PII SVMP homologues
disintegrin domain
zinc-binding motif
hemorrhagic activity
platelet aggregation
proteinase activity
title_short Novel Catalytically-Inactive PII Metalloproteinases from a Viperid Snake Venom with Substitutions in the Canonical Zinc-Binding Motif
title_full Novel Catalytically-Inactive PII Metalloproteinases from a Viperid Snake Venom with Substitutions in the Canonical Zinc-Binding Motif
title_fullStr Novel Catalytically-Inactive PII Metalloproteinases from a Viperid Snake Venom with Substitutions in the Canonical Zinc-Binding Motif
title_full_unstemmed Novel Catalytically-Inactive PII Metalloproteinases from a Viperid Snake Venom with Substitutions in the Canonical Zinc-Binding Motif
title_sort Novel Catalytically-Inactive PII Metalloproteinases from a Viperid Snake Venom with Substitutions in the Canonical Zinc-Binding Motif
author Camacho, Erika
author_facet Camacho, Erika
Sanz, Libia
Escalante, Teresa
Pérez, Alicia
Villalta, Fabián
Lomonte, Bruno
Ferreira, Ana Gisele C. Neves
Feoli, Andrés
Calvete, Juan J.
Gutiérrez, José María
Rucavado, Alexandra
author_role author
author2 Sanz, Libia
Escalante, Teresa
Pérez, Alicia
Villalta, Fabián
Lomonte, Bruno
Ferreira, Ana Gisele C. Neves
Feoli, Andrés
Calvete, Juan J.
Gutiérrez, José María
Rucavado, Alexandra
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Camacho, Erika
Sanz, Libia
Escalante, Teresa
Pérez, Alicia
Villalta, Fabián
Lomonte, Bruno
Ferreira, Ana Gisele C. Neves
Feoli, Andrés
Calvete, Juan J.
Gutiérrez, José María
Rucavado, Alexandra
dc.subject.other.pt_BR.fl_str_mv Metaloproteinases de veneno de cobra
Domínio da desintegração
Motivo de ligação ao zinco
Atividade hemorrágica
Agregação de plaquetas
Atividade de proteinase
topic Metaloproteinases de veneno de cobra
Domínio da desintegração
Motivo de ligação ao zinco
Atividade hemorrágica
Agregação de plaquetas
Atividade de proteinase
snake venom metalloproteinases
PII SVMP homologues
disintegrin domain
zinc-binding motif
hemorrhagic activity
platelet aggregation
proteinase activity
dc.subject.en.pt_BR.fl_str_mv snake venom metalloproteinases
PII SVMP homologues
disintegrin domain
zinc-binding motif
hemorrhagic activity
platelet aggregation
proteinase activity
description Universidad de Costa Rica. Instituto Clodomiro Picado. Facultad de Microbiologia. San Jose, Costa, Rica.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-03-13T13:26:02Z
dc.date.available.fl_str_mv 2017-03-13T13:26:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CAMACHO, Erika; et al. Novel Catalytically-Inactive PII Metalloproteinases from a Viperid Snake Venom with Substitutions in the Canonical Zinc-Binding Motif. Toxins (Basel), v.8, n.10:292,18 p, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18016
dc.identifier.issn.pt_BR.fl_str_mv 2072-6651
dc.identifier.doi.none.fl_str_mv 10.3390/toxins8100292
dc.identifier.eissn.none.fl_str_mv 2072-6651
identifier_str_mv CAMACHO, Erika; et al. Novel Catalytically-Inactive PII Metalloproteinases from a Viperid Snake Venom with Substitutions in the Canonical Zinc-Binding Motif. Toxins (Basel), v.8, n.10:292,18 p, 2016.
2072-6651
10.3390/toxins8100292
url https://www.arca.fiocruz.br/handle/icict/18016
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/18016/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/18016/2/anagisele_ferreira_etal_IOC_2016.pdf
https://www.arca.fiocruz.br/bitstream/icict/18016/3/anagisele_ferreira_etal_IOC_2016.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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