miR-548d-3p Alters Parasite Growth and Inflammation in Leishmania (Viannia) braziliensis Infection

Detalhes bibliográficos
Autor(a) principal: Souza, Marina de Assis
Data de Publicação: 2021
Outros Autores: Ramos-Sanchez, Eduardo Milton, Muxel, Sandra Márcia, Lagos, Dimitris, Reis, Luiza Campos, Pereira, Valéria Rêgo Alves, Brito, Maria Edileuza Felinto, Zampieri, Ricardo Andrade, Kaye, Paul Martin, Floeter-Winter, Lucile Maria, Goto, Hiro
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/51464
Resumo: Universidade de São Paulo. Faculdade de Medicina. Instituto de Medicina Tropical. São Paulo, SP, Brazil.
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spelling Souza, Marina de AssisRamos-Sanchez, Eduardo MiltonMuxel, Sandra MárciaLagos, DimitrisReis, Luiza CamposPereira, Valéria Rêgo AlvesBrito, Maria Edileuza FelintoZampieri, Ricardo AndradeKaye, Paul MartinFloeter-Winter, Lucile MariaGoto, Hiro2022-02-24T14:50:02Z2022-02-24T14:50:02Z2021SOUZA, Marina de Assis et al. miR-548d-3p Alters Parasite Growth and Inflammation in Leishmania (Viannia) braziliensis Infection. Frontiers in Cellular and Infection Microbiology, v. 11, Article 687647, p. 1-15, June 2021.2235-2988https://www.arca.fiocruz.br/handle/icict/5146410.3389/fcimb.2021.687647porAnimalsBrazilHumansInflammationLeishmania braziliensisMicroRNAsParasitesTHP-1 cellsLeishmania braziliensismicroRNAPathogenesisActive cutaneous leishmaniasisSelf-healed cutaneous leishmaniasismiR-548d-3p Alters Parasite Growth and Inflammation in Leishmania (Viannia) braziliensis Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de São Paulo. Faculdade de Medicina. Instituto de Medicina Tropical. São Paulo, SP, Brazil.Universidade de São Paulo. Faculdade de Medicina. Instituto de Medicina Tropical. São Paulo, SP, Brazil / Universidad Nacional Toribio Rodriguez de Mendoza de Amazonas. Facultad de Ciencias de La Salud. Departamento de Salud Publica. Departamento de Salud Publica. Chachapoyas, Peru.Universidade de São Paulo. Instituto de Biociências. São Paulo, SP, Brazil.University of York, York. Hull York Medical School. York Biomedical Research Institute. London, United Kingdom.Universidade de São Paulo. Faculdade de Medicina. Instituto de Medicina Tropical. São Paulo, SP, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brazil.Universidade de São Paulo. Instituto de Biociências. São Paulo, SP, Brazil.University of York, York. Hull York Medical School. York Biomedical Research Institute. London, United Kingdom.Universidade de São Paulo. Instituto de Biociências. São Paulo, SP, Brazil.Universidade de São Paulo. Faculdade de Medicina. Instituto de Medicina Tropical. São Paulo, SP, Brazil / Universidade de São Paulo. Faculdade de Medicina. Departamento de Medicina Preventiva. São Paulo, SP, Brazil.American Tegumentary Leishmaniasis (ATL) is an endemic disease in Latin America, mainly caused in Brazil by Leishmania (Viannia) braziliensis. Clinical manifestations vary from mild, localized cutaneous leishmaniasis (CL) to aggressive mucosal disease. The host immune response strongly determines the outcome of infection and pattern of disease. However, the pathogenesis of ATL is not well understood, and host microRNAs (miRNAs) may have a role in this context. In the present study, miRNAs were quantified using qPCR arrays in human monocytic THP-1 cells infected in vitro with L. (V.) braziliensis promastigotes and in plasma from patients with ATL, focusing on inflammatory response-specific miRNAs. Patients with active or self-healed cutaneous leishmaniasis patients, with confirmed parasitological or immunological diagnosis, were compared with healthy controls. Computational target prediction of significantly-altered miRNAs from in vitro L. (V.) braziliensis-infected THP-1 cells revealed predicted targets involved in diverse pathways, including chemokine signaling, inflammatory, cellular proliferation, and tissue repair processes. In plasma, we observed distinct miRNA expression in patients with self-healed and active lesions compared with healthy controls. Some miRNAs dysregulated during THP-1 in vitro infection were also found in plasma from self-healed patients, including miR-548d-3p, which was upregulated in infected THP-1 cells and in plasma from self-healed patients. As miR-548d-3p was predicted to target the chemokine pathway and inflammation is a central to the pathogenesis of ATL, we evaluated the effect of transient transfection of a miR-548d-3p inhibitor on L. (V.) braziliensis infected-THP-1 cells. Inhibition of miR-548d-3p reduced parasite growth early after infection and increased production of MCP1/CCL2, RANTES/CCL5, and IP10/CXCL10. In plasma of self-healed patients, MCP1/CCL2, RANTES/CCL5, and IL-8/CXCL8 concentrations were significantly decreased and MIG/CXCL9 and IP-10/CXCL10 increased compared to patients with active disease. These data suggest that by modulating miRNAs, L. (V.) braziliensis may interfere with chemokine production and hence the inflammatory processes underpinning lesion resolution. Our data suggest miR-548d-3p could be further evaluated as a prognostic marker for ATL and/or as a host-directed therapeutic target.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83088https://www.arca.fiocruz.br/bitstream/icict/51464/1/license.txt7b043faf2a6ad46e5344ca8d54801d8cMD51ORIGINALmiR-548d-3p Alters Parasite Growth and Inflammation in Leishmania (Viannia) braziliensis Infection.pdfmiR-548d-3p Alters Parasite Growth and Inflammation in Leishmania (Viannia) braziliensis Infection.pdfapplication/pdf4406539https://www.arca.fiocruz.br/bitstream/icict/51464/2/miR-548d-3p%20Alters%20Parasite%20Growth%20and%20Inflammation%20in%20Leishmania%20%28Viannia%29%20braziliensis%20Infection.pdf4ab2da016db8615ef0962c1dd7321982MD52icict/514642022-02-28 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dc.title.pt_BR.fl_str_mv miR-548d-3p Alters Parasite Growth and Inflammation in Leishmania (Viannia) braziliensis Infection
title miR-548d-3p Alters Parasite Growth and Inflammation in Leishmania (Viannia) braziliensis Infection
spellingShingle miR-548d-3p Alters Parasite Growth and Inflammation in Leishmania (Viannia) braziliensis Infection
Souza, Marina de Assis
Animals
Brazil
Humans
Inflammation
Leishmania braziliensis
MicroRNAs
Parasites
THP-1 cells
Leishmania braziliensis
microRNA
Pathogenesis
Active cutaneous leishmaniasis
Self-healed cutaneous leishmaniasis
title_short miR-548d-3p Alters Parasite Growth and Inflammation in Leishmania (Viannia) braziliensis Infection
title_full miR-548d-3p Alters Parasite Growth and Inflammation in Leishmania (Viannia) braziliensis Infection
title_fullStr miR-548d-3p Alters Parasite Growth and Inflammation in Leishmania (Viannia) braziliensis Infection
title_full_unstemmed miR-548d-3p Alters Parasite Growth and Inflammation in Leishmania (Viannia) braziliensis Infection
title_sort miR-548d-3p Alters Parasite Growth and Inflammation in Leishmania (Viannia) braziliensis Infection
author Souza, Marina de Assis
author_facet Souza, Marina de Assis
Ramos-Sanchez, Eduardo Milton
Muxel, Sandra Márcia
Lagos, Dimitris
Reis, Luiza Campos
Pereira, Valéria Rêgo Alves
Brito, Maria Edileuza Felinto
Zampieri, Ricardo Andrade
Kaye, Paul Martin
Floeter-Winter, Lucile Maria
Goto, Hiro
author_role author
author2 Ramos-Sanchez, Eduardo Milton
Muxel, Sandra Márcia
Lagos, Dimitris
Reis, Luiza Campos
Pereira, Valéria Rêgo Alves
Brito, Maria Edileuza Felinto
Zampieri, Ricardo Andrade
Kaye, Paul Martin
Floeter-Winter, Lucile Maria
Goto, Hiro
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Marina de Assis
Ramos-Sanchez, Eduardo Milton
Muxel, Sandra Márcia
Lagos, Dimitris
Reis, Luiza Campos
Pereira, Valéria Rêgo Alves
Brito, Maria Edileuza Felinto
Zampieri, Ricardo Andrade
Kaye, Paul Martin
Floeter-Winter, Lucile Maria
Goto, Hiro
dc.subject.mesh.pt_BR.fl_str_mv Animals
Brazil
Humans
Inflammation
Leishmania braziliensis
MicroRNAs
Parasites
THP-1 cells
topic Animals
Brazil
Humans
Inflammation
Leishmania braziliensis
MicroRNAs
Parasites
THP-1 cells
Leishmania braziliensis
microRNA
Pathogenesis
Active cutaneous leishmaniasis
Self-healed cutaneous leishmaniasis
dc.subject.en.pt_BR.fl_str_mv Leishmania braziliensis
microRNA
Pathogenesis
Active cutaneous leishmaniasis
Self-healed cutaneous leishmaniasis
description Universidade de São Paulo. Faculdade de Medicina. Instituto de Medicina Tropical. São Paulo, SP, Brazil.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-02-24T14:50:02Z
dc.date.available.fl_str_mv 2022-02-24T14:50:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUZA, Marina de Assis et al. miR-548d-3p Alters Parasite Growth and Inflammation in Leishmania (Viannia) braziliensis Infection. Frontiers in Cellular and Infection Microbiology, v. 11, Article 687647, p. 1-15, June 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/51464
dc.identifier.issn.pt_BR.fl_str_mv 2235-2988
dc.identifier.doi.pt_BR.fl_str_mv 10.3389/fcimb.2021.687647
identifier_str_mv SOUZA, Marina de Assis et al. miR-548d-3p Alters Parasite Growth and Inflammation in Leishmania (Viannia) braziliensis Infection. Frontiers in Cellular and Infection Microbiology, v. 11, Article 687647, p. 1-15, June 2021.
2235-2988
10.3389/fcimb.2021.687647
url https://www.arca.fiocruz.br/handle/icict/51464
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/51464/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/51464/2/miR-548d-3p%20Alters%20Parasite%20Growth%20and%20Inflammation%20in%20Leishmania%20%28Viannia%29%20braziliensis%20Infection.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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