Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter?

Detalhes bibliográficos
Autor(a) principal: Dias, Luciana dos Santos
Data de Publicação: 2018
Outros Autores: Bauzer, Luíz Guilherme Soares da Rocha, Lima, José Bento Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31754
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, Rio de Janeiro, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil.
id CRUZ_7fe43cef106a7751eee54d873bb6fcc9
oai_identifier_str oai:www.arca.fiocruz.br:icict/31754
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Dias, Luciana dos SantosBauzer, Luíz Guilherme Soares da RochaLima, José Bento Pereira2019-02-19T14:45:49Z2019-02-19T14:45:49Z2018DIAS, Luciana dos Santos; BAUZER, Luíz Guilherme Soares da Rocha; LIMA, José Bento Pereira. Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter? Revista do Instituto de Medicina Tropical de São Paulo, São Paulo, v. 60, p. 1-11, 2018.0036-4665https://www.arca.fiocruz.br/handle/icict/3175410.1590/s1678-99462018600451678-9946engUniversidade de São PauloCulicidaeColonizaçãoAlimentação artificial de sangueAnticoagulantesBem estar animalCulicidaeColonizationArtificial blood feedingAnticoagulantsAnimal welfareArtificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter?info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, Rio de Janeiro, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, Rio de Janeiro, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, Rio de Janeiro, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil.Culicidae colonization in laboratory is paramount to conduct studies aiming at a better understanding of mosquitoes' capacity to transmit pathogens that cause deadly diseases. Colonization requires female blood feeding, a necessary step for maturation of female's oocytes. Direct blood feeding on anesthetized mammals implies in a number of disadvantages when compared to artificial blood feeding. Consequently, laboratories worldwide have been trying to -feed female mosquitoes artificially in order to replace direct feeding. In this study, we compared the effects of direct blood feeding and artificial blood feeding on important life traits of three Culicidae species. Artificial feeding was performed using citrated or defibrinated sheep blood and citrated or defibrinated rabbit blood. Direct feeding was performed using anesthetized guinea pigs as the blood source and the experiment control. Results indicated that artificial feeding using sheep blood was not good enough to justify its use in the maintenance of laboratory colonies of Culicidae. However, artificial feeding using rabbit blood maintained a recovery rate always very close to the control, especially when blood was citrated. We concluded that artificial feeding using citrated rabbit blood can substitute direct feeding on mammals reducing the use of animals, eliminating the need to maintain a bioterium in the laboratory and reducing costs in scientific researches involving Culicidae vectors.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/31754/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjosebentopereiralima_etal_IOC_2018.pdfjosebentopereiralima_etal_IOC_2018.pdfapplication/pdf1790576https://www.arca.fiocruz.br/bitstream/icict/31754/2/josebentopereiralima_etal_IOC_2018.pdf9d438e45ffc398732ebc45794aae09b9MD52TEXTjosebentopereiralima_etal_IOC_2018.pdf.txtjosebentopereiralima_etal_IOC_2018.pdf.txtExtracted texttext/plain45522https://www.arca.fiocruz.br/bitstream/icict/31754/3/josebentopereiralima_etal_IOC_2018.pdf.txtb72cbb2d5e5c5c52bbe059337289ffedMD53icict/317542019-02-22 23:38:40.647oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-02-23T02:38:40Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter?
title Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter?
spellingShingle Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter?
Dias, Luciana dos Santos
Culicidae
Colonização
Alimentação artificial de sangue
Anticoagulantes
Bem estar animal
Culicidae
Colonization
Artificial blood feeding
Anticoagulants
Animal welfare
title_short Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter?
title_full Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter?
title_fullStr Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter?
title_full_unstemmed Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter?
title_sort Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter?
author Dias, Luciana dos Santos
author_facet Dias, Luciana dos Santos
Bauzer, Luíz Guilherme Soares da Rocha
Lima, José Bento Pereira
author_role author
author2 Bauzer, Luíz Guilherme Soares da Rocha
Lima, José Bento Pereira
author2_role author
author
dc.contributor.author.fl_str_mv Dias, Luciana dos Santos
Bauzer, Luíz Guilherme Soares da Rocha
Lima, José Bento Pereira
dc.subject.other.pt_BR.fl_str_mv Culicidae
Colonização
Alimentação artificial de sangue
Anticoagulantes
Bem estar animal
topic Culicidae
Colonização
Alimentação artificial de sangue
Anticoagulantes
Bem estar animal
Culicidae
Colonization
Artificial blood feeding
Anticoagulants
Animal welfare
dc.subject.en.pt_BR.fl_str_mv Culicidae
Colonization
Artificial blood feeding
Anticoagulants
Animal welfare
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, Rio de Janeiro, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-02-19T14:45:49Z
dc.date.available.fl_str_mv 2019-02-19T14:45:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DIAS, Luciana dos Santos; BAUZER, Luíz Guilherme Soares da Rocha; LIMA, José Bento Pereira. Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter? Revista do Instituto de Medicina Tropical de São Paulo, São Paulo, v. 60, p. 1-11, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31754
dc.identifier.issn.pt_BR.fl_str_mv 0036-4665
dc.identifier.doi.none.fl_str_mv 10.1590/s1678-9946201860045
dc.identifier.eissn.none.fl_str_mv 1678-9946
identifier_str_mv DIAS, Luciana dos Santos; BAUZER, Luíz Guilherme Soares da Rocha; LIMA, José Bento Pereira. Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter? Revista do Instituto de Medicina Tropical de São Paulo, São Paulo, v. 60, p. 1-11, 2018.
0036-4665
10.1590/s1678-9946201860045
1678-9946
url https://www.arca.fiocruz.br/handle/icict/31754
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/31754/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/31754/2/josebentopereiralima_etal_IOC_2018.pdf
https://www.arca.fiocruz.br/bitstream/icict/31754/3/josebentopereiralima_etal_IOC_2018.pdf.txt
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
9d438e45ffc398732ebc45794aae09b9
b72cbb2d5e5c5c52bbe059337289ffed
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324716414435328

Registros relacionados