The Kunitz chymotrypsin inhibitor from Erythrina velutina seeds displays activity against HeLa cells through arrest in cell cycle
| Autor(a) principal: | |
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| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/57247 |
Resumo: | Universidade Federal do Rio Grande do Norte. Departamento de Bioquímica. Centro de Biociências. Laboratório de Química e Função de Proteínas Bioativas. Natal, RN, Brasil / Instituto Federal de Ciências e Tecnologia de Mato Grosso-IFMT. Cuiabá, MT, Brasil. |
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Lucena, Sheyla V.Rufino, Fabíola P.Moura, Gioconda Emanuella Diniz de DantasRabêlo, Luciana M. A.Monteiro, Norberto K. V.Ferreira, André T.Perales, Jonas E. AguilarUchôa, Adriana F.Justo, Giselle Z.Oliveira, Caio F. R. deMigliolo, LudovicoNader, Elizeu A.Santos, Elizeu A.Oliveira, Adeliana S.2023-03-05T19:17:02Z2023-03-05T19:17:02Z2022LUCENA, Sheyla V. et al. The Kunitz chymotrypsin inhibitor from Erythrina velutina seeds displays activity against HeLa cells through arrest in cell cycle. Biotech, v.3, 12:19, p. 1 - 10, 2022.2571-5135https://www.arca.fiocruz.br/handle/icict/5724710.1007/s13205-021-03084-0engSpringerCélula HeLaCâncer cervicalApoptoseInibidor de quimotripsinaSementes de leguminosasEvCIEvCIHeLa cellCervical cancerApoptosisChymotrypsin inhibitorLeguminous seedsThe Kunitz chymotrypsin inhibitor from Erythrina velutina seeds displays activity against HeLa cells through arrest in cell cycleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio Grande do Norte. Departamento de Bioquímica. Centro de Biociências. Laboratório de Química e Função de Proteínas Bioativas. Natal, RN, Brasil / Instituto Federal de Ciências e Tecnologia de Mato Grosso-IFMT. Cuiabá, MT, Brasil.Universidade Federal do Rio Grande do Norte. Departamento de Bioquímica. Centro de Biociências. Laboratório de Química e Função de Proteínas Bioativas. Natal, RN, Brasil.Universidade Federal de São Paulo (UNIFESP). Departamento de Bioquimica. São Paulo, SP, Brasil.Universidade Federal do Rio Grande do Norte. Departamento de Bioquímica. Centro de Biociências. Laboratório de Química e Função de Proteínas Bioativas. Natal, RN, Brasil.Universidade Federal do Rio Grande do Norte. Departamento de Bioquímica. Centro de Biociências. Laboratório de Química e Função de Proteínas Bioativas. Natal, RN, Brasil.Universidade Federal do Rio Grande do Norte. Departamento de Bioquímica. Centro de Biociências. Laboratório de Química e Função de Proteínas Bioativas. Natal, RN, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Grande do Norte. Departamento de Bioquímica. Centro de Biociências. Laboratório de Química e Função de Proteínas Bioativas. Natal, RN, Brasil / Universidade Federal de São Paulo (UNIFESP). Departamento de Bioquímica. São Paulo, SP, Brasil / Universidade Federal do Rio Grande do Norte.Centro de Biociências. Instituto de Medicina Tropical do Rio Grande do Norte. Laboratório de Proteômica. Natal, RN, Brasil.Universidade Federal de São Paulo (UNIFESP). Departamento de Bioquímica. São Paulo, SP, Brasil / UNIFESP. Departamento de Ciências Biológicas. Diadema, SP, Brasil.Universidade Federal de Mato Grosso do Sul. Laboratório de Purificação de Proteínas e suas Funções Biológicas. Campo Grande, MS, Brasil.Universidade Federal do Rio Grande do Norte. Departamento de Bioquímica. Centro de Biociências. Laboratório de Química e Função de Proteínas Bioativas. Natal, RN, Brasil / S‑Inova Biotech, Programa de Pós‑Graduação em Biotecnologia, Universidade Católica Dom Bosco. Campo Grande, MS, Brasil.Universidade Federal de São Paulo (UNIFESP). Departamento de Bioquímica. São Paulo, SP, Brasil.Universidade Federal do Rio Grande do Norte. Departamento de Bioquímica. Centro de Biociências. Laboratório de Química e Função de Proteínas Bioativas. Natal, RN, Brasil / Universidade Federal do Rio Grande do Norte. Laboratório de Proteômica, Instituto de Medicina Tropical do Rio Grande do Norte, Centro de Biociências. Natal, RN, Brasil.Universidade Federal do Rio Grande do Norte. Departamento de Bioquímica. Centro de Biociências. Laboratório de Química e Função de Proteínas Bioativas. Natal, RN, Brasil.Erythrina velutina is a species of arboreal leguminous that occurs spontaneously in the northeastern states of Brazil. Leguminous seeds represent an abundant source of peptidase inhibitors, which play an important role in controlling peptidases involved in essential biological processes. The aim of this study was to purify and characterize a novel Kunitz-type peptidase inhibitor from Erythrina velutina seeds and evaluate its anti-proliferative effects against cancer cell lines. The Kunitz-type chymotrypsin inhibitor was purified from Erythrina velutina seeds (EvCI) by ammonium sulphate fractionation, trypsin– and chymotrypsin–sepharose affinity chromatographies and Resource Q anion-exchange column. The purified EvCI has a molecular mass of 18 kDa with homology to a Kunitz-type inhibitor. Inhibition assays revealed that EvCI is a competitive inhibitor of chymotrypsin (with Ki of 4 × 10– 8 M), with weak inhibitory activity against human elastase and without inhibition against trypsin, elastase, bromelain or papain. In addition, the inhibitory activity of EvCI was stable over a wide range of pH and temperature. Disulfide bridges are involved in stabilization of the reactive site in EvCI, since the reduction of disulfide bridges with DTT 100 mM abolished ~ 50% of its inhibitory activity. The inhibitor exhibited selective anti-proliferative properties against HeLa cells. The incubation of EvCI with HeLa cells triggered arrest in the cell cycle, suggesting that apoptosis is the mechanism of death induced by the inhibitor. EvCI constitutes an interesting anti-carcinogenic candidate for conventional cervical cancer treatments employed currently. The EvCI cytostatic effect on Hela cells indicates a promised compound to be used as anti-carcinogenic complement for conventional cervical treatments employed currently.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/57247/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALJOnas Perales_etal_IOC_2022.pdfJOnas Perales_etal_IOC_2022.pdfapplication/pdf1138051https://www.arca.fiocruz.br/bitstream/icict/57247/2/JOnas%20Perales_etal_IOC_2022.pdf28143507ef06d6acf821e3fd20f149edMD52icict/572472023-09-04 11:30:09.665oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-04T14:30:09Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
The Kunitz chymotrypsin inhibitor from Erythrina velutina seeds displays activity against HeLa cells through arrest in cell cycle |
| title |
The Kunitz chymotrypsin inhibitor from Erythrina velutina seeds displays activity against HeLa cells through arrest in cell cycle |
| spellingShingle |
The Kunitz chymotrypsin inhibitor from Erythrina velutina seeds displays activity against HeLa cells through arrest in cell cycle Lucena, Sheyla V. Célula HeLa Câncer cervical Apoptose Inibidor de quimotripsina Sementes de leguminosas EvCI EvCI HeLa cell Cervical cancer Apoptosis Chymotrypsin inhibitor Leguminous seeds |
| title_short |
The Kunitz chymotrypsin inhibitor from Erythrina velutina seeds displays activity against HeLa cells through arrest in cell cycle |
| title_full |
The Kunitz chymotrypsin inhibitor from Erythrina velutina seeds displays activity against HeLa cells through arrest in cell cycle |
| title_fullStr |
The Kunitz chymotrypsin inhibitor from Erythrina velutina seeds displays activity against HeLa cells through arrest in cell cycle |
| title_full_unstemmed |
The Kunitz chymotrypsin inhibitor from Erythrina velutina seeds displays activity against HeLa cells through arrest in cell cycle |
| title_sort |
The Kunitz chymotrypsin inhibitor from Erythrina velutina seeds displays activity against HeLa cells through arrest in cell cycle |
| author |
Lucena, Sheyla V. |
| author_facet |
Lucena, Sheyla V. Rufino, Fabíola P. Moura, Gioconda Emanuella Diniz de Dantas Rabêlo, Luciana M. A. Monteiro, Norberto K. V. Ferreira, André T. Perales, Jonas E. Aguilar Uchôa, Adriana F. Justo, Giselle Z. Oliveira, Caio F. R. de Migliolo, Ludovico Nader, Elizeu A. Santos, Elizeu A. Oliveira, Adeliana S. |
| author_role |
author |
| author2 |
Rufino, Fabíola P. Moura, Gioconda Emanuella Diniz de Dantas Rabêlo, Luciana M. A. Monteiro, Norberto K. V. Ferreira, André T. Perales, Jonas E. Aguilar Uchôa, Adriana F. Justo, Giselle Z. Oliveira, Caio F. R. de Migliolo, Ludovico Nader, Elizeu A. Santos, Elizeu A. Oliveira, Adeliana S. |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lucena, Sheyla V. Rufino, Fabíola P. Moura, Gioconda Emanuella Diniz de Dantas Rabêlo, Luciana M. A. Monteiro, Norberto K. V. Ferreira, André T. Perales, Jonas E. Aguilar Uchôa, Adriana F. Justo, Giselle Z. Oliveira, Caio F. R. de Migliolo, Ludovico Nader, Elizeu A. Santos, Elizeu A. Oliveira, Adeliana S. |
| dc.subject.other.en_US.fl_str_mv |
Célula HeLa Câncer cervical Apoptose Inibidor de quimotripsina Sementes de leguminosas EvCI |
| topic |
Célula HeLa Câncer cervical Apoptose Inibidor de quimotripsina Sementes de leguminosas EvCI EvCI HeLa cell Cervical cancer Apoptosis Chymotrypsin inhibitor Leguminous seeds |
| dc.subject.en.en_US.fl_str_mv |
EvCI HeLa cell Cervical cancer Apoptosis Chymotrypsin inhibitor Leguminous seeds |
| description |
Universidade Federal do Rio Grande do Norte. Departamento de Bioquímica. Centro de Biociências. Laboratório de Química e Função de Proteínas Bioativas. Natal, RN, Brasil / Instituto Federal de Ciências e Tecnologia de Mato Grosso-IFMT. Cuiabá, MT, Brasil. |
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2022 |
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2022 |
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2023-03-05T19:17:02Z |
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2023-03-05T19:17:02Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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LUCENA, Sheyla V. et al. The Kunitz chymotrypsin inhibitor from Erythrina velutina seeds displays activity against HeLa cells through arrest in cell cycle. Biotech, v.3, 12:19, p. 1 - 10, 2022. |
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https://www.arca.fiocruz.br/handle/icict/57247 |
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2571-5135 |
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10.1007/s13205-021-03084-0 |
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LUCENA, Sheyla V. et al. The Kunitz chymotrypsin inhibitor from Erythrina velutina seeds displays activity against HeLa cells through arrest in cell cycle. Biotech, v.3, 12:19, p. 1 - 10, 2022. 2571-5135 10.1007/s13205-021-03084-0 |
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Springer |
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Springer |
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