Leishmania amazonensis: Biological and biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities.

Detalhes bibliográficos
Autor(a) principal: Pinheiro, Carla Monica
Data de Publicação: 2006
Outros Autores: Duarte, Erica dos Santos Martins, Ferraro, Rodrigo Barbosa, Souza, André Luíz Fonseca de, Gomes, Marta T, Lopes, Angela H.C.S, Santos, Marcos André Vannier dos, Santos, André L.S, Fernandes, José Roberto Meyer
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11842
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Centro de Ciências da Saúde. Rio de Janeiro, RJ, Brasil
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spelling Pinheiro, Carla MonicaDuarte, Erica dos Santos MartinsFerraro, Rodrigo BarbosaSouza, André Luíz Fonseca deGomes, Marta TLopes, Angela H.C.SSantos, Marcos André Vannier dosSantos, André L.SFernandes, José Roberto Meyer2015-09-29T16:08:29Z2015-09-29T16:08:29Z2006PINHEIRO, C. M. et al. Leishmania amazonensis: Biological and biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities. Experimental Parasitology, v. 114, n. 1, p. 16-25, 2006.0014-4894https://www.arca.fiocruz.br/handle/icict/1184210.1016/j.exppara.2006.02.007engElsevierLeishmania amazonensis: Biological and biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Centro de Ciências da Saúde. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Centro de Ciências da Saúde. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Centro de Ciências da Saúde. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Centro de Ciências da Saúde. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Centro de Ciências da Saúde. Rio de Janeiro, RJ, BrasilThe presence of Leishmania amazonensis ecto-nucleoside triphosphate triphosphohydrolase activities was demonstrated using antibodies against different NTPDase members by Western blotting, flow cytometry, and immunoelectron microscopy analysis. Living promastigote cells sequentially hydrolyzed the ATP molecule generating ADP, AMP, and adenosine, indicating that this surface enzyme may play a role in the salvage of purines from the extracellular medium. The L. amazonensis ecto-NTPDase activities were insensitive to Triton X-100, but they were enhanced by divalent cations, such as Mg(2+). In addition, the ecto-NTPDase activities decreased with time for 96 h when promastigotes were grown in vitro. On the other hand, these activities increased considerably when measured in living amastigote forms. Furthermore, the treatment with adenosine, a mediator of several relevant biological phenomena, induced a decrease in the reactivity with anti-CD39 antibody, raised against mammalian E-NTPDase, probably because of down regulation in the L. amazonensis ecto-NTPDase expression. Also, adenosine and anti-NTPDase antibodies induced a significant diminishing in the interaction between promastigotes of L. amazonensis and mouse peritoneal macrophages.Leishmania amazonensisEcto-ATPaseAdenosineE-NTPDaseAdenosina Trifosfatases/metabolismoLeishmania mexicana/enzimologiaDifosfato de Adenosina/metabolismoMonofosfato de Adenosina/metabolismoTrifosfato de Adenosina/metabolismoAnimaisAntígenos CD/metabolismoApirase/metabolismoWestern BlottingFemininoCitometria de FluxoHumanosLeishmaniose Cutânea/parasitologiaMacrófagos Peritoneais/parasitologiaCamundongosMicroscopia ImunoeletrônicaPirofosfatases/metabolismoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALPinheiro CL Leishmania....pdfPinheiro CL Leishmania....pdfapplication/pdf699593https://www.arca.fiocruz.br/bitstream/icict/11842/2/Pinheiro%20CL%20Leishmania....pdf0388d5cd0021553b77b5437f2f674237MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/11842/3/license.txt8b4c200b4e10021c5683c6ccaba07169MD53TEXTPinheiro CL Leishmania....pdf.txtPinheiro CL Leishmania....pdf.txtExtracted texttext/plain46418https://www.arca.fiocruz.br/bitstream/icict/11842/4/Pinheiro%20CL%20Leishmania....pdf.txta34df55afe5058c5067835324465998fMD54icict/118422023-03-15 14:33:10.08oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:10Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Leishmania amazonensis: Biological and biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities.
title Leishmania amazonensis: Biological and biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities.
spellingShingle Leishmania amazonensis: Biological and biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities.
Pinheiro, Carla Monica
Leishmania amazonensis
Ecto-ATPase
Adenosine
E-NTPDase
Adenosina Trifosfatases/metabolismo
Leishmania mexicana/enzimologia
Difosfato de Adenosina/metabolismo
Monofosfato de Adenosina/metabolismo
Trifosfato de Adenosina/metabolismo
Animais
Antígenos CD/metabolismo
Apirase/metabolismo
Western Blotting
Feminino
Citometria de Fluxo
Humanos
Leishmaniose Cutânea/parasitologia
Macrófagos Peritoneais/parasitologia
Camundongos
Microscopia Imunoeletrônica
Pirofosfatases/metabolismo
title_short Leishmania amazonensis: Biological and biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities.
title_full Leishmania amazonensis: Biological and biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities.
title_fullStr Leishmania amazonensis: Biological and biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities.
title_full_unstemmed Leishmania amazonensis: Biological and biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities.
title_sort Leishmania amazonensis: Biological and biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities.
author Pinheiro, Carla Monica
author_facet Pinheiro, Carla Monica
Duarte, Erica dos Santos Martins
Ferraro, Rodrigo Barbosa
Souza, André Luíz Fonseca de
Gomes, Marta T
Lopes, Angela H.C.S
Santos, Marcos André Vannier dos
Santos, André L.S
Fernandes, José Roberto Meyer
author_role author
author2 Duarte, Erica dos Santos Martins
Ferraro, Rodrigo Barbosa
Souza, André Luíz Fonseca de
Gomes, Marta T
Lopes, Angela H.C.S
Santos, Marcos André Vannier dos
Santos, André L.S
Fernandes, José Roberto Meyer
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pinheiro, Carla Monica
Duarte, Erica dos Santos Martins
Ferraro, Rodrigo Barbosa
Souza, André Luíz Fonseca de
Gomes, Marta T
Lopes, Angela H.C.S
Santos, Marcos André Vannier dos
Santos, André L.S
Fernandes, José Roberto Meyer
dc.subject.en.pt_BR.fl_str_mv Leishmania amazonensis
Ecto-ATPase
Adenosine
E-NTPDase
topic Leishmania amazonensis
Ecto-ATPase
Adenosine
E-NTPDase
Adenosina Trifosfatases/metabolismo
Leishmania mexicana/enzimologia
Difosfato de Adenosina/metabolismo
Monofosfato de Adenosina/metabolismo
Trifosfato de Adenosina/metabolismo
Animais
Antígenos CD/metabolismo
Apirase/metabolismo
Western Blotting
Feminino
Citometria de Fluxo
Humanos
Leishmaniose Cutânea/parasitologia
Macrófagos Peritoneais/parasitologia
Camundongos
Microscopia Imunoeletrônica
Pirofosfatases/metabolismo
dc.subject.decs.pt_BR.fl_str_mv Adenosina Trifosfatases/metabolismo
Leishmania mexicana/enzimologia
Difosfato de Adenosina/metabolismo
Monofosfato de Adenosina/metabolismo
Trifosfato de Adenosina/metabolismo
Animais
Antígenos CD/metabolismo
Apirase/metabolismo
Western Blotting
Feminino
Citometria de Fluxo
Humanos
Leishmaniose Cutânea/parasitologia
Macrófagos Peritoneais/parasitologia
Camundongos
Microscopia Imunoeletrônica
Pirofosfatases/metabolismo
description Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Centro de Ciências da Saúde. Rio de Janeiro, RJ, Brasil
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2015-09-29T16:08:29Z
dc.date.available.fl_str_mv 2015-09-29T16:08:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PINHEIRO, C. M. et al. Leishmania amazonensis: Biological and biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities. Experimental Parasitology, v. 114, n. 1, p. 16-25, 2006.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11842
dc.identifier.issn.none.fl_str_mv 0014-4894
dc.identifier.doi.none.fl_str_mv 10.1016/j.exppara.2006.02.007
identifier_str_mv PINHEIRO, C. M. et al. Leishmania amazonensis: Biological and biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities. Experimental Parasitology, v. 114, n. 1, p. 16-25, 2006.
0014-4894
10.1016/j.exppara.2006.02.007
url https://www.arca.fiocruz.br/handle/icict/11842
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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