A dhfr-ts- Leishmania major knockout mutant cross-protects against Leishmania amazonensis.

Detalhes bibliográficos
Autor(a) principal: Veras, Patrícia Sampaio Tavares
Data de Publicação: 1999
Outros Autores: Brodskyn, Claudia Ida, Balestieri, Filomena Maria Perrella, Freitas, Luiz Antonio Rodrigues de, Ramos, A. P. S, Queiroz, A. R. P, Barral, Aldina Maria Prado, Beverley, Stephen M, Barral Netto, Manoel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7444
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil
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spelling Veras, Patrícia Sampaio TavaresBrodskyn, Claudia IdaBalestieri, Filomena Maria PerrellaFreitas, Luiz Antonio Rodrigues deRamos, A. P. SQueiroz, A. R. PBarral, Aldina Maria PradoBeverley, Stephen MBarral Netto, Manoel2014-03-25T17:21:29Z2014-03-25T17:21:29Z1999VERAS, P. S. R. et al. A dhfr-ts- Leishmania major knockout mutant cross-protects against Leishmania amazonensis. Memórias do Instituto Oswaldo Cruz, v. 94, n. 4, p. 491-496, 1999.0074-0276https://www.arca.fiocruz.br/handle/icict/7444engFiocruzA dhfr-ts- Leishmania major knockout mutant cross-protects against Leishmania amazonensis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Laboratório de Patologia e Biologia Celular. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. Departamento de Fisiologia e Patologia. João Pessoa, PB, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilWashington University School of Medicine. Department of Molecular Microbiology. St. Louis, MO, USAFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imuno-regulação e Microbiologia. Salvador, BA, BrasilE10-5A3 is a dhfr-ts- Leishmania major double knockout auxotrophic shown previously to induce substantial protection against virulent L. major infection in both genetically susceptible and resistant mice. We investigated the capacity of dhfr-ts- to protect against heterologous infection by L. amazonensis. The degree of protection was evaluated by immunization of BALB/c or C57BL/6 mice with E10-5A3, followed by L. amazonensis challenge. Whether immunized by subcutaneous (SC) or intravenous (IV) inoculation, susceptible and resistant mice displayed a partial degree of protection against challenge with virulent L. amazonensis. SC-immunized BALB/c mice developed lesions 40 to 65% smaller than non immunized mice, while IV immunization led to protection ranging from 40 to 75% in four out of six experiments compared to non immunized animals. The resistant C57BL/6 mice displayed comparable degrees of protection, 57% by SC and 49% by IV immunization. Results are encouraging as it has been previously difficult to obtain protection by SC vaccination against Leishmania, the preferred route for human immunization.Auxotrophic Leishmania majorLeishmania amazonensisImmunizationCrossProtectionAntígenos de Protozoários/administração & dosagemLeishmania major/imunologiaLeishmaniose Cutânea/prevenção & controleComplexos Multienzimáticos/imunologiaVacinas Protozoárias/imunologiaTetra-Hidrofolato Desidrogenase/imunologiaTimidilato Sintase/imunologiaAnimaisLeishmaniose Cutânea/imunologiaCamundongosCamundongos Endogâmicos BALB CCamundongos KnockoutCamundongos Mutantesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALVeras P S T A DHFR-TS-Leishmania....pdfVeras P S T A DHFR-TS-Leishmania....pdfapplication/pdf110138https://www.arca.fiocruz.br/bitstream/icict/7444/1/Veras%20P%20S%20T%20%20%20A%20DHFR-TS-Leishmania....pdf9ff4af91314a1a31c562511b75155ddfMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7444/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTVeras P S T A DHFR-TS-Leishmania....pdf.txtVeras P S T A DHFR-TS-Leishmania....pdf.txtExtracted texttext/plain23654https://www.arca.fiocruz.br/bitstream/icict/7444/5/Veras%20P%20S%20T%20%20%20A%20DHFR-TS-Leishmania....pdf.txt411d1e49ac5945ad37af10e7db9cfee2MD55THUMBNAILVeras P S T A DHFR-TS-Leishmania....pdf.jpgVeras P S T A DHFR-TS-Leishmania....pdf.jpgGenerated Thumbnailimage/jpeg2338https://www.arca.fiocruz.br/bitstream/icict/7444/4/Veras%20P%20S%20T%20%20%20A%20DHFR-TS-Leishmania....pdf.jpg39ac362f70988a87e5b911666ca909f3MD54icict/74442023-03-15 14:33:01.703oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:01Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv A dhfr-ts- Leishmania major knockout mutant cross-protects against Leishmania amazonensis.
title A dhfr-ts- Leishmania major knockout mutant cross-protects against Leishmania amazonensis.
spellingShingle A dhfr-ts- Leishmania major knockout mutant cross-protects against Leishmania amazonensis.
Veras, Patrícia Sampaio Tavares
Auxotrophic Leishmania major
Leishmania amazonensis
Immunization
Cross
Protection
Antígenos de Protozoários/administração & dosagem
Leishmania major/imunologia
Leishmaniose Cutânea/prevenção & controle
Complexos Multienzimáticos/imunologia
Vacinas Protozoárias/imunologia
Tetra-Hidrofolato Desidrogenase/imunologia
Timidilato Sintase/imunologia
Animais
Leishmaniose Cutânea/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Knockout
Camundongos Mutantes
title_short A dhfr-ts- Leishmania major knockout mutant cross-protects against Leishmania amazonensis.
title_full A dhfr-ts- Leishmania major knockout mutant cross-protects against Leishmania amazonensis.
title_fullStr A dhfr-ts- Leishmania major knockout mutant cross-protects against Leishmania amazonensis.
title_full_unstemmed A dhfr-ts- Leishmania major knockout mutant cross-protects against Leishmania amazonensis.
title_sort A dhfr-ts- Leishmania major knockout mutant cross-protects against Leishmania amazonensis.
author Veras, Patrícia Sampaio Tavares
author_facet Veras, Patrícia Sampaio Tavares
Brodskyn, Claudia Ida
Balestieri, Filomena Maria Perrella
Freitas, Luiz Antonio Rodrigues de
Ramos, A. P. S
Queiroz, A. R. P
Barral, Aldina Maria Prado
Beverley, Stephen M
Barral Netto, Manoel
author_role author
author2 Brodskyn, Claudia Ida
Balestieri, Filomena Maria Perrella
Freitas, Luiz Antonio Rodrigues de
Ramos, A. P. S
Queiroz, A. R. P
Barral, Aldina Maria Prado
Beverley, Stephen M
Barral Netto, Manoel
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Veras, Patrícia Sampaio Tavares
Brodskyn, Claudia Ida
Balestieri, Filomena Maria Perrella
Freitas, Luiz Antonio Rodrigues de
Ramos, A. P. S
Queiroz, A. R. P
Barral, Aldina Maria Prado
Beverley, Stephen M
Barral Netto, Manoel
dc.subject.en.pt_BR.fl_str_mv Auxotrophic Leishmania major
Leishmania amazonensis
Immunization
Cross
Protection
topic Auxotrophic Leishmania major
Leishmania amazonensis
Immunization
Cross
Protection
Antígenos de Protozoários/administração & dosagem
Leishmania major/imunologia
Leishmaniose Cutânea/prevenção & controle
Complexos Multienzimáticos/imunologia
Vacinas Protozoárias/imunologia
Tetra-Hidrofolato Desidrogenase/imunologia
Timidilato Sintase/imunologia
Animais
Leishmaniose Cutânea/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Knockout
Camundongos Mutantes
dc.subject.decs.pt_BR.fl_str_mv Antígenos de Protozoários/administração & dosagem
Leishmania major/imunologia
Leishmaniose Cutânea/prevenção & controle
Complexos Multienzimáticos/imunologia
Vacinas Protozoárias/imunologia
Tetra-Hidrofolato Desidrogenase/imunologia
Timidilato Sintase/imunologia
Animais
Leishmaniose Cutânea/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Knockout
Camundongos Mutantes
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil
publishDate 1999
dc.date.issued.fl_str_mv 1999
dc.date.accessioned.fl_str_mv 2014-03-25T17:21:29Z
dc.date.available.fl_str_mv 2014-03-25T17:21:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VERAS, P. S. R. et al. A dhfr-ts- Leishmania major knockout mutant cross-protects against Leishmania amazonensis. Memórias do Instituto Oswaldo Cruz, v. 94, n. 4, p. 491-496, 1999.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7444
dc.identifier.issn.none.fl_str_mv 0074-0276
identifier_str_mv VERAS, P. S. R. et al. A dhfr-ts- Leishmania major knockout mutant cross-protects against Leishmania amazonensis. Memórias do Instituto Oswaldo Cruz, v. 94, n. 4, p. 491-496, 1999.
0074-0276
url https://www.arca.fiocruz.br/handle/icict/7444
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fiocruz
publisher.none.fl_str_mv Fiocruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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