Is pannexin the pore associated with the P2X7 receptor?

Detalhes bibliográficos
Autor(a) principal: Alberto, Anael Viana Pinto
Data de Publicação: 2013
Outros Autores: Faria, R. X., Couto, C. G. C., Ferreira, L. G. B., Souza, C. A. M., Teixeira, P. C. N., Fróes, M. M., Alves, L. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11721
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.
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spelling Alberto, Anael Viana PintoFaria, R. X.Couto, C. G. C.Ferreira, L. G. B.Souza, C. A. M.Teixeira, P. C. N.Fróes, M. M.Alves, L. A.2015-09-21T17:25:29Z2015-09-21T17:25:29Z2013ALBERTO, A. V. P.; et al. Is pannexin the pore associated with the P2X7 receptor?. Naunyn-Schmiedeberg's Arch Pharmacol, v.386, p..775–787, 2013.https://www.arca.fiocruz.br/handle/icict/1172110.1007/s00210-013-0868-xengSpringer-Verlag Berlin HeidelbergIs pannexin the pore associated with the P2X7 receptor?info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Ciências Biomédicas. Laboratório de Neuroanatomia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.The P2X7 receptor (P2X7R), an ATP-gated cation channel, is expressed predominantly in leukocytes. Activation of P2X7R has been implicated in the formation of a cytolytic pore (i.e., a large conductance channel) that allows the passage of molecules up to 900 Da in macrophages. At least two hypotheses have been presented to explain the conversion of a nonselective cation channel to a cytolytic pore. One hypothesis suggests that the pore is a separate molecular structure activated by P2X7R, and the second asserts that this is an intrinsic property of P2X7R (pore dilation). Based on connexin knockout and hemichannel antagonist studies, some groups have concluded that connexins and pannexins, the hemichannel-forming proteins in vertebrates, are fundamental components of the large conductance channel associated with P2X7R. Dye uptake and electrophysiology experiments were used to evaluate the efficacy and specificity of some hemichannel antagonists under conditions known to open the large conductance channel associated with P2X7R. Hemichannel antagonists and interference RNA (RNAi) targeting pannexin-1 did not affect P2X7R macroscopic currents [ATP, 1,570±189 pA; ATP+100 μMcarbenoxolone (CBX), 1,498±100 pA; ATP+1 mM probenecid (Prob), 1,522 ±9 pA] or dye uptake in a FACS assay (ATP, 63±5 %; ATP+ 100 μM CBX, 51.51±8.4 %; ATP+1 mM Prob, 57.7±4.3 %) in mousemacrophages. These findings strongly suggest that the high-permeability pore evident after prolonged P2X7R activation does not occur through connexin or pannexin hemichannels in murine macrophages. Another membrane protein may be involved in P2X7R pore formation.ConnexinPannexinHemichannelsGap junctionsP2X7 receptorGap junction antagonistsElectrophysiologyPorePorinasConnexina 43Junções gapEletrofisiologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11721/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALcgc_couto_etal_IOC-2013.pdfapplication/pdf1191915https://www.arca.fiocruz.br/bitstream/icict/11721/2/cgc_couto_etal_IOC-2013.pdf754a81dd2727f8b56c2add42ec566705MD52TEXTcgc_couto_etal_IOC-2013.pdf.txtcgc_couto_etal_IOC-2013.pdf.txtExtracted texttext/plain52058https://www.arca.fiocruz.br/bitstream/icict/11721/3/cgc_couto_etal_IOC-2013.pdf.txt8e84db340837f8df6780b35c42473362MD53icict/117212022-06-24 12:17:31.693oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:17:31Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Is pannexin the pore associated with the P2X7 receptor?
title Is pannexin the pore associated with the P2X7 receptor?
spellingShingle Is pannexin the pore associated with the P2X7 receptor?
Alberto, Anael Viana Pinto
Connexin
Pannexin
Hemichannels
Gap junctions
P2X7 receptor
Gap junction antagonists
Electrophysiology
Pore
Porinas
Connexina 43
Junções gap
Eletrofisiologia
title_short Is pannexin the pore associated with the P2X7 receptor?
title_full Is pannexin the pore associated with the P2X7 receptor?
title_fullStr Is pannexin the pore associated with the P2X7 receptor?
title_full_unstemmed Is pannexin the pore associated with the P2X7 receptor?
title_sort Is pannexin the pore associated with the P2X7 receptor?
author Alberto, Anael Viana Pinto
author_facet Alberto, Anael Viana Pinto
Faria, R. X.
Couto, C. G. C.
Ferreira, L. G. B.
Souza, C. A. M.
Teixeira, P. C. N.
Fróes, M. M.
Alves, L. A.
author_role author
author2 Faria, R. X.
Couto, C. G. C.
Ferreira, L. G. B.
Souza, C. A. M.
Teixeira, P. C. N.
Fróes, M. M.
Alves, L. A.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alberto, Anael Viana Pinto
Faria, R. X.
Couto, C. G. C.
Ferreira, L. G. B.
Souza, C. A. M.
Teixeira, P. C. N.
Fróes, M. M.
Alves, L. A.
dc.subject.en.pt_BR.fl_str_mv Connexin
Pannexin
Hemichannels
Gap junctions
P2X7 receptor
Gap junction antagonists
Electrophysiology
Pore
topic Connexin
Pannexin
Hemichannels
Gap junctions
P2X7 receptor
Gap junction antagonists
Electrophysiology
Pore
Porinas
Connexina 43
Junções gap
Eletrofisiologia
dc.subject.decs.pt_BR.fl_str_mv Porinas
Connexina 43
Junções gap
Eletrofisiologia
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-09-21T17:25:29Z
dc.date.available.fl_str_mv 2015-09-21T17:25:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALBERTO, A. V. P.; et al. Is pannexin the pore associated with the P2X7 receptor?. Naunyn-Schmiedeberg's Arch Pharmacol, v.386, p..775–787, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11721
dc.identifier.doi.pt_BR.fl_str_mv 10.1007/s00210-013-0868-x
identifier_str_mv ALBERTO, A. V. P.; et al. Is pannexin the pore associated with the P2X7 receptor?. Naunyn-Schmiedeberg's Arch Pharmacol, v.386, p..775–787, 2013.
10.1007/s00210-013-0868-x
url https://www.arca.fiocruz.br/handle/icict/11721
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer-Verlag Berlin Heidelberg
publisher.none.fl_str_mv Springer-Verlag Berlin Heidelberg
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/11721/2/cgc_couto_etal_IOC-2013.pdf
https://www.arca.fiocruz.br/bitstream/icict/11721/3/cgc_couto_etal_IOC-2013.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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