An Outbreak of Gastroenteritis Associated with Astrovirus Serotype 1 in a Day Care Center, in Rio de Janeiro, Brazil

Detalhes bibliográficos
Autor(a) principal: Silva, A. M. V.
Data de Publicação: 2001
Outros Autores: Leite, E. G., Assis, R. M. S., Majerowicz, S., Leite, José Paulo G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44161
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.
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spelling Silva, A. M. V.Leite, E. G.Assis, R. M. S.Majerowicz, S.Leite, José Paulo G.2020-10-24T14:19:03Z2020-10-24T14:19:03Z2001SILVA, A. M. V. et al. An Outbreak of Gastroenteritis Associated with Astrovirus Serotype 1 in a Day Care Center, in Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 8, p. 1069-1073, Nov. 2001.0074-0206https://www.arca.fiocruz.br/handle/icict/4416110.1590/S0074-027620010008000071678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.AstrovirusGastroenteriteSurtoRio de JaneiroAstrovirusGastroenteritis -OutbreakRio de JaneiroBrazilAn Outbreak of Gastroenteritis Associated with Astrovirus Serotype 1 in a Day Care Center, in Rio de Janeiro, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.Between June 4th and June 20th 1996 rotavirus, adenovirus, and astrovirus (HAstrV) were investigated in fecal samples from 27 children under three years old with acute diarrhea, attending the Bertha Lutz day care center, in Rio de Janeiro. All fecal samples were analyzed by polyacrylamide gel electrophoresis (PAGE), reverse transcriptase polymerase chain reaction (RT-PCR), enzyme immunoassays (EIA), and electron microscopy (EM). Nine of them (33%) showed positive results for HAstrV by at least one of the employed methodologies. Eight were positive by RT-PCR and EIA, and six by EM. All positive samples were inoculated onto HT-29 (human colon adenocarcinoma) cultured cells for HAstrV isolation and seven were positive after three passages. The sequencing analysis of eight RT-PCR products (449 bp) from gene that codifies VP2 protein, showed a total nucleotide identity among them and 98% with HAstrV-1 (strain Oxford type 1). This is the first report of a gastroenteritis outbreak associated with HAstrv-1 in a day care center in Rio de Janeiro and it reinforces the importance of this virus in association with infantile acute gastroenteritis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44161/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAMVSilva_JPLeite_etal_IOC_2001.pdfAMVSilva_JPLeite_etal_IOC_2001.pdfapplication/pdf145538https://www.arca.fiocruz.br/bitstream/icict/44161/2/AMVSilva_JPLeite_etal_IOC_2001.pdfc6f8f288d6e19923216bc6beabf953b5MD52TEXTAMVSilva_JPLeite_etal_IOC_2001.pdf.txtAMVSilva_JPLeite_etal_IOC_2001.pdf.txtExtracted texttext/plain19633https://www.arca.fiocruz.br/bitstream/icict/44161/3/AMVSilva_JPLeite_etal_IOC_2001.pdf.txt01714eafe5b5832b22f1e5d8ea4f139aMD53icict/441612023-09-05 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dc.title.pt_BR.fl_str_mv An Outbreak of Gastroenteritis Associated with Astrovirus Serotype 1 in a Day Care Center, in Rio de Janeiro, Brazil
title An Outbreak of Gastroenteritis Associated with Astrovirus Serotype 1 in a Day Care Center, in Rio de Janeiro, Brazil
spellingShingle An Outbreak of Gastroenteritis Associated with Astrovirus Serotype 1 in a Day Care Center, in Rio de Janeiro, Brazil
Silva, A. M. V.
Astrovirus
Gastroenterite
Surto
Rio de Janeiro
Astrovirus
Gastroenteritis -
Outbreak
Rio de Janeiro
Brazil
title_short An Outbreak of Gastroenteritis Associated with Astrovirus Serotype 1 in a Day Care Center, in Rio de Janeiro, Brazil
title_full An Outbreak of Gastroenteritis Associated with Astrovirus Serotype 1 in a Day Care Center, in Rio de Janeiro, Brazil
title_fullStr An Outbreak of Gastroenteritis Associated with Astrovirus Serotype 1 in a Day Care Center, in Rio de Janeiro, Brazil
title_full_unstemmed An Outbreak of Gastroenteritis Associated with Astrovirus Serotype 1 in a Day Care Center, in Rio de Janeiro, Brazil
title_sort An Outbreak of Gastroenteritis Associated with Astrovirus Serotype 1 in a Day Care Center, in Rio de Janeiro, Brazil
author Silva, A. M. V.
author_facet Silva, A. M. V.
Leite, E. G.
Assis, R. M. S.
Majerowicz, S.
Leite, José Paulo G.
author_role author
author2 Leite, E. G.
Assis, R. M. S.
Majerowicz, S.
Leite, José Paulo G.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Silva, A. M. V.
Leite, E. G.
Assis, R. M. S.
Majerowicz, S.
Leite, José Paulo G.
dc.subject.other.pt_BR.fl_str_mv Astrovirus
Gastroenterite
Surto
Rio de Janeiro
topic Astrovirus
Gastroenterite
Surto
Rio de Janeiro
Astrovirus
Gastroenteritis -
Outbreak
Rio de Janeiro
Brazil
dc.subject.en.pt_BR.fl_str_mv Astrovirus
Gastroenteritis -
Outbreak
Rio de Janeiro
Brazil
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2020-10-24T14:19:03Z
dc.date.available.fl_str_mv 2020-10-24T14:19:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, A. M. V. et al. An Outbreak of Gastroenteritis Associated with Astrovirus Serotype 1 in a Day Care Center, in Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 8, p. 1069-1073, Nov. 2001.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44161
dc.identifier.issn.pt_BR.fl_str_mv 0074-0206
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762001000800007
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv SILVA, A. M. V. et al. An Outbreak of Gastroenteritis Associated with Astrovirus Serotype 1 in a Day Care Center, in Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 8, p. 1069-1073, Nov. 2001.
0074-0206
10.1590/S0074-02762001000800007
1678-8060
url https://www.arca.fiocruz.br/handle/icict/44161
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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