Toward a novel experimental model of infection to study American cutaneous leishmania braziliensis

Detalhes bibliográficos
Autor(a) principal: Moura, Tatiana Rodrigues de
Data de Publicação: 2005
Outros Autores: Novais, Fernanda Oliveira, Oliveira, Fabiano, Clarêncio, Jorge, Almério, Noronha, Barral, Aldina Maria Prado, Brodskyn, Claudia Ida, Oliveira, Camila Indiani de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/2819
Resumo: Fundacação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
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spelling Moura, Tatiana Rodrigues deNovais, Fernanda OliveiraOliveira, FabianoClarêncio, JorgeAlmério, NoronhaBarral, Aldina Maria PradoBrodskyn, Claudia IdaOliveira, Camila Indiani de2011-08-30T16:52:14Z2011-08-30T16:52:14Z2005MOURA, T. R. de et al. Toward a novel experimental model of infection to study American cutaneous leishmania braziliensis. Infectium Immunity, v.73, n.9, p.5827-34, 2005.doi:10.1128/IAI.73.9.5827–5834.2005https://www.arca.fiocruz.br/handle/icict/28191098-5522engAnimaisLinfócitos T CD4-PositivosLinfócitos T CD8-PositivosQuimiocinas CCDerme/PS/PAModelos Animais de DoençasOrelha Externa/IM/PS/PAFemininoInterferon gama/MELeishmania braziliensisLeishmaniose CutâneaLinfonodos/ME/PS/PACamundongosCamundongos Endogâmicos BALB CToward a novel experimental model of infection to study American cutaneous leishmania braziliensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundacação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundacação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundacação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundacação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundacação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundacação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundacação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundacação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilLeishmania spp. cause a broad spectrum of diseases collectively known as leishmaniasis. Leishmania braziliensis is the main etiological agent of American cutaneous leishmaniasis (ACL) and mucocutaneous leishmaniasis. In the present study, we have developed an experimental model of infection that closely resembles ACL caused by L. braziliensis. In order to do so, BALB/c mice were infected in the ear dermis with 105 parasites and distinct aspects of the infection were evaluated. Following inoculation, parasite expansion in the ear dermis was accompanied by the development of an ulcerated dermal lesion which healed spontaneously, as seen by the presence of a scar. Histological analysis of infected ears showed the presence of a mixed inflammatory infiltrate consisting of both mononuclear and polymorphonuclear cells. In draining lymph nodes, parasite replication was detected throughout the infection. In vitro restimulation of draining lymph node cells followed by intracellular staining showed an up-regulation in the production of gamma interferon (IFN- ) and in the frequency of IFN- -secreting CD4 and CD8 T cells. Reverse transcription-PCR of ears and draining lymph node cells showed the expression of CC chemokines. The dermal model of infection with L. braziliensis herein is able to reproduce aspects of the natural infection, such as the presence of an ulcerated lesion, parasite dissemination to lymphoid areas, and the development of a Th1-type immune response. These results indicate that this model shall be useful to address questions related to the concomitant immunity to reinfection and parasite persistence leading to mucocutaneous leishmaniasisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALDe Moura TR Toward a novel experimental....pdfDe Moura TR Toward a novel experimental....pdfapplication/pdf348196https://www.arca.fiocruz.br/bitstream/icict/2819/1/De%20Moura%20TR%20Toward%20a%20novel%20experimental....pdf46e6d367b869b076aa9aed7f6778f310MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/2819/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTDe Moura TR Toward a novel experimental....pdf.txtDe Moura TR Toward a novel experimental....pdf.txtExtracted texttext/plain43864https://www.arca.fiocruz.br/bitstream/icict/2819/5/De%20Moura%20TR%20Toward%20a%20novel%20experimental....pdf.txtf5d609f55924fcf90de3e7f5391d5739MD55THUMBNAILDe Moura TR Toward a novel experimental....pdf.jpgDe Moura TR Toward a novel experimental....pdf.jpgGenerated Thumbnailimage/jpeg2150https://www.arca.fiocruz.br/bitstream/icict/2819/4/De%20Moura%20TR%20Toward%20a%20novel%20experimental....pdf.jpg16001a222db235a2fa77faa3add73673MD54icict/28192023-03-15 14:32:59.567oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:59Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Toward a novel experimental model of infection to study American cutaneous leishmania braziliensis
title Toward a novel experimental model of infection to study American cutaneous leishmania braziliensis
spellingShingle Toward a novel experimental model of infection to study American cutaneous leishmania braziliensis
Moura, Tatiana Rodrigues de
Animais
Linfócitos T CD4-Positivos
Linfócitos T CD8-Positivos
Quimiocinas CC
Derme/PS/PA
Modelos Animais de Doenças
Orelha Externa/IM/PS/PA
Feminino
Interferon gama/ME
Leishmania braziliensis
Leishmaniose Cutânea
Linfonodos/ME/PS/PA
Camundongos
Camundongos Endogâmicos BALB C
title_short Toward a novel experimental model of infection to study American cutaneous leishmania braziliensis
title_full Toward a novel experimental model of infection to study American cutaneous leishmania braziliensis
title_fullStr Toward a novel experimental model of infection to study American cutaneous leishmania braziliensis
title_full_unstemmed Toward a novel experimental model of infection to study American cutaneous leishmania braziliensis
title_sort Toward a novel experimental model of infection to study American cutaneous leishmania braziliensis
author Moura, Tatiana Rodrigues de
author_facet Moura, Tatiana Rodrigues de
Novais, Fernanda Oliveira
Oliveira, Fabiano
Clarêncio, Jorge
Almério, Noronha
Barral, Aldina Maria Prado
Brodskyn, Claudia Ida
Oliveira, Camila Indiani de
author_role author
author2 Novais, Fernanda Oliveira
Oliveira, Fabiano
Clarêncio, Jorge
Almério, Noronha
Barral, Aldina Maria Prado
Brodskyn, Claudia Ida
Oliveira, Camila Indiani de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Moura, Tatiana Rodrigues de
Novais, Fernanda Oliveira
Oliveira, Fabiano
Clarêncio, Jorge
Almério, Noronha
Barral, Aldina Maria Prado
Brodskyn, Claudia Ida
Oliveira, Camila Indiani de
dc.subject.other.pt_BR.fl_str_mv Animais
Linfócitos T CD4-Positivos
Linfócitos T CD8-Positivos
Quimiocinas CC
Derme/PS/PA
Modelos Animais de Doenças
Orelha Externa/IM/PS/PA
Feminino
Interferon gama/ME
Leishmania braziliensis
Leishmaniose Cutânea
Linfonodos/ME/PS/PA
Camundongos
Camundongos Endogâmicos BALB C
topic Animais
Linfócitos T CD4-Positivos
Linfócitos T CD8-Positivos
Quimiocinas CC
Derme/PS/PA
Modelos Animais de Doenças
Orelha Externa/IM/PS/PA
Feminino
Interferon gama/ME
Leishmania braziliensis
Leishmaniose Cutânea
Linfonodos/ME/PS/PA
Camundongos
Camundongos Endogâmicos BALB C
description Fundacação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2011-08-30T16:52:14Z
dc.date.available.fl_str_mv 2011-08-30T16:52:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MOURA, T. R. de et al. Toward a novel experimental model of infection to study American cutaneous leishmania braziliensis. Infectium Immunity, v.73, n.9, p.5827-34, 2005.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/2819
dc.identifier.issn.none.fl_str_mv doi:10.1128/IAI.73.9.5827–5834.2005
dc.identifier.doi.none.fl_str_mv 1098-5522
identifier_str_mv MOURA, T. R. de et al. Toward a novel experimental model of infection to study American cutaneous leishmania braziliensis. Infectium Immunity, v.73, n.9, p.5827-34, 2005.
doi:10.1128/IAI.73.9.5827–5834.2005
1098-5522
url https://www.arca.fiocruz.br/handle/icict/2819
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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