Combination of Mass Cytometry and Imaging Analysis Reveals Origin, Location, and Functional Repopulation of Liver Myeloid Cells in Mice

David, Bruna Araujo; Rezende, Rafael Machado; Antunes, Maísa Mota; Santos, Mônica Morais; Lopes, Maria Alice Freitas; Diniz, Ariane Barros; Pereira, Rafaela Vaz Sousa; Marchesi, Sarah Cozzer; Alvarenga, Débora Moreira; Nakagaki, Brenda Naemi; Araújo, Alan Moreira; Reis, Daniela Silva dos; Rocha, Renata Monti; Marques, Pedro Elias; Lee, Woo-Yong; Deniset, Justin; Liew, Pei Xiong; Rubino, Stephen; Cox, Laura; Pinho, Vanessa; Cunha, Thiago Mattar; Fernandes, Gabriel Rocha; Oliveira, André Gustavo; Teixeira, Mauro Martins; Kubes, Paul; Menezes, Gustavo Batista

Combination of Mass Cytometry and Imaging Analysis Reveals Origin, Location, and Functional Repopulation of Liver Myeloid Cells in Mice

Detalhes bibliográficos
Autor(a) principal:	David, Bruna Araujo
Data de Publicação:	2016
Outros Autores:	Rezende, Rafael Machado, Antunes, Maísa Mota, Santos, Mônica Morais, Lopes, Maria Alice Freitas, Diniz, Ariane Barros, Pereira, Rafaela Vaz Sousa, Marchesi, Sarah Cozzer, Alvarenga, Débora Moreira, Nakagaki, Brenda Naemi, Araújo, Alan Moreira, Reis, Daniela Silva dos, Rocha, Renata Monti, Marques, Pedro Elias, Lee, Woo-Yong, Deniset, Justin, Liew, Pei Xiong, Rubino, Stephen, Cox, Laura, Pinho, Vanessa, Cunha, Thiago Mattar, Fernandes, Gabriel Rocha, Oliveira, André Gustavo, Teixeira, Mauro Martins, Kubes, Paul, Menezes, Gustavo Batista
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/23531
Resumo:	2021-01-01

Metadados do item

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network_acronym_str	CRUZ
network_name_str	Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str	2135
spelling	David, Bruna AraujoRezende, Rafael MachadoAntunes, Maísa MotaSantos, Mônica MoraisLopes, Maria Alice FreitasDiniz, Ariane BarrosPereira, Rafaela Vaz SousaMarchesi, Sarah CozzerAlvarenga, Débora MoreiraNakagaki, Brenda NaemiAraújo, Alan MoreiraReis, Daniela Silva dosRocha, Renata MontiMarques, Pedro EliasLee, Woo-YongDeniset, JustinLiew, Pei XiongRubino, StephenCox, LauraPinho, VanessaCunha, Thiago MattarFernandes, Gabriel RochaOliveira, André GustavoTeixeira, Mauro MartinsKubes, PaulMenezes, Gustavo Batista2017-12-06T18:43:34Z2017-12-06T18:43:34Z2016DAVID, Bruna Araujo et al. Combination of Mass Cytometry and Imaging Analysis Reveals Origin, Location, and Functional Repopulation of Liver Myeloid Cells in Mice. Gastroenterology. v. 151, n. 6, p. 1176-1191, 20160016-5085https://www.arca.fiocruz.br/handle/icict/2353110.1053/j.gastro.2016.08.024engElsevier Inc.CyTOFDCCyTOFDCLiver DevelopmentMouse ModelCombination of Mass Cytometry and Imaging Analysis Reveals Origin, Location, and Functional Repopulation of Liver Myeloid Cells in Miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2021-01-01Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Centro de Biologia Gastrointestinal. Belo Horizonte, MG, BrazilHarvard Medical School, Brigham and Women’s Hospital. Ann Romney Center for Neurologic Diseases. Boston, MA, United States of America.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Centro de Biologia Gastrointestinal. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Centro de Biologia Gastrointestinal. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Centro de Biologia Gastrointestinal. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Centro de Biologia Gastrointestinal. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Centro de Biologia Gastrointestinal. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Centro de Biologia Gastrointestinal. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Centro de Biologia Gastrointestinal. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Centro de Biologia Gastrointestinal. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Centro de Biologia Gastrointestinal. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais. Belo Horizonte, MG, BrazilUniversity of Calgary. Alberta, CanadaUniversity of Calgary. Alberta, CanadaUniversity of Calgary. Alberta, CanadaHarvard Medical School, Brigham and Women’s Hospital. Ann Romney Center for Neurologic Diseases. Boston, MA, United States of America.Harvard Medical School, Brigham and Women’s Hospital. Ann Romney Center for Neurologic Diseases. Boston, MA, United States of America.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Resolução de Inflamação. Belo Horizonte, MG, BrazilUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. São Paulo, SP, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Centro de Biologia Gastrointestinal. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Fisiologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais. Belo Horizonte, MG, BrazilUniversity of Calgary. Alberta, CanadaUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Centro de Biologia Gastrointestinal. Belo Horizonte, MG, BrazilBACKGROUND & AIMS: Resident macrophages are derived from yolk sac precursors and seed the liver during embryogenesis. Native cells may be replaced by bone marrow precursors during extensive injuries, irradiation, and infections. We investigated the liver populations of myeloid immune cells and their location, as well as the dynamics of phagocyte repopulation after full depletion. The effects on liver function due to the substitution of original phagocytes by bone marrow-derived surrogates were also examined. METHODS: We collected and analyzed liver tissues from C57BL/6 (control), LysM-EGFP, B6 ACTb-EGFP, CCR2-/-, CD11c-EYFP, CD11c-EYFP-DTR, germ-free mice, CX3CR1gfp/gfp, CX3CR1gpf/wt, and CX3CR1-DTR-EYFP. Liver nonparenchymal cells were immunophenotyped using mass cytometry and gene expression analyses. Kupffer and dendritic cells were depleted from mice by administration of clodronate, and their location and phenotype were examined using intravital microscopy and time-of-flight mass cytometry. Mice were given acetaminophen gavage or intravenous injections of fluorescently labeled Escherichia coli, blood samples were collected and analyzed, and liver function was evaluated. We assessed cytokine profiles of liver tissues using a multiplexed array. RESULTS: Using mass cytometry and gene expression analyses, we identified 2 populations of hepatic macrophages and 2 populations of monocytes. We also identified 4 populations of dendritic cells and 1 population of basophils. After selective depletion of liver phagocytes, intravascular myeloid precursors began to differentiate into macrophages and dendritic cells; dendritic cells migrated out of sinusoids, after a delay, via the chemokine CX3CL1. The cell distribution returned to normal in 2 weeks, but the repopulated livers were unable to fully respond to drug-induced injury or clear bacteria for at least 1 month. This defect was associated with increased levels of inflammatory cytokines, and dexamethasone accelerated the repopulation of liver phagocytes. CONCLUSIONS: In studies of hepatic phagocyte depletion in mice, we found that myeloid precursors can differentiate into liver macrophages and dendritic cells, which each localize to distinct tissue compartments. 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dc.title.pt_BR.fl_str_mv	Combination of Mass Cytometry and Imaging Analysis Reveals Origin, Location, and Functional Repopulation of Liver Myeloid Cells in Mice
title	Combination of Mass Cytometry and Imaging Analysis Reveals Origin, Location, and Functional Repopulation of Liver Myeloid Cells in Mice
spellingShingle	Combination of Mass Cytometry and Imaging Analysis Reveals Origin, Location, and Functional Repopulation of Liver Myeloid Cells in Mice David, Bruna Araujo CyTOF DC CyTOF DC Liver Development Mouse Model
title_short	Combination of Mass Cytometry and Imaging Analysis Reveals Origin, Location, and Functional Repopulation of Liver Myeloid Cells in Mice
title_full	Combination of Mass Cytometry and Imaging Analysis Reveals Origin, Location, and Functional Repopulation of Liver Myeloid Cells in Mice
title_fullStr	Combination of Mass Cytometry and Imaging Analysis Reveals Origin, Location, and Functional Repopulation of Liver Myeloid Cells in Mice
title_full_unstemmed	Combination of Mass Cytometry and Imaging Analysis Reveals Origin, Location, and Functional Repopulation of Liver Myeloid Cells in Mice
title_sort	Combination of Mass Cytometry and Imaging Analysis Reveals Origin, Location, and Functional Repopulation of Liver Myeloid Cells in Mice
author	David, Bruna Araujo
author_facet	David, Bruna Araujo Rezende, Rafael Machado Antunes, Maísa Mota Santos, Mônica Morais Lopes, Maria Alice Freitas Diniz, Ariane Barros Pereira, Rafaela Vaz Sousa Marchesi, Sarah Cozzer Alvarenga, Débora Moreira Nakagaki, Brenda Naemi Araújo, Alan Moreira Reis, Daniela Silva dos Rocha, Renata Monti Marques, Pedro Elias Lee, Woo-Yong Deniset, Justin Liew, Pei Xiong Rubino, Stephen Cox, Laura Pinho, Vanessa Cunha, Thiago Mattar Fernandes, Gabriel Rocha Oliveira, André Gustavo Teixeira, Mauro Martins Kubes, Paul Menezes, Gustavo Batista
author_role	author
author2	Rezende, Rafael Machado Antunes, Maísa Mota Santos, Mônica Morais Lopes, Maria Alice Freitas Diniz, Ariane Barros Pereira, Rafaela Vaz Sousa Marchesi, Sarah Cozzer Alvarenga, Débora Moreira Nakagaki, Brenda Naemi Araújo, Alan Moreira Reis, Daniela Silva dos Rocha, Renata Monti Marques, Pedro Elias Lee, Woo-Yong Deniset, Justin Liew, Pei Xiong Rubino, Stephen Cox, Laura Pinho, Vanessa Cunha, Thiago Mattar Fernandes, Gabriel Rocha Oliveira, André Gustavo Teixeira, Mauro Martins Kubes, Paul Menezes, Gustavo Batista
author2_role	author author author author author author author author author author author author author author author author author author author author author author author author author
dc.contributor.author.fl_str_mv	David, Bruna Araujo Rezende, Rafael Machado Antunes, Maísa Mota Santos, Mônica Morais Lopes, Maria Alice Freitas Diniz, Ariane Barros Pereira, Rafaela Vaz Sousa Marchesi, Sarah Cozzer Alvarenga, Débora Moreira Nakagaki, Brenda Naemi Araújo, Alan Moreira Reis, Daniela Silva dos Rocha, Renata Monti Marques, Pedro Elias Lee, Woo-Yong Deniset, Justin Liew, Pei Xiong Rubino, Stephen Cox, Laura Pinho, Vanessa Cunha, Thiago Mattar Fernandes, Gabriel Rocha Oliveira, André Gustavo Teixeira, Mauro Martins Kubes, Paul Menezes, Gustavo Batista
dc.subject.other.pt_BR.fl_str_mv	CyTOF DC
topic	CyTOF DC CyTOF DC Liver Development Mouse Model
dc.subject.en.pt_BR.fl_str_mv	CyTOF DC Liver Development Mouse Model
description	2021-01-01
publishDate	2016
dc.date.issued.fl_str_mv	2016
dc.date.accessioned.fl_str_mv	2017-12-06T18:43:34Z
dc.date.available.fl_str_mv	2017-12-06T18:43:34Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	DAVID, Bruna Araujo et al. Combination of Mass Cytometry and Imaging Analysis Reveals Origin, Location, and Functional Repopulation of Liver Myeloid Cells in Mice. Gastroenterology. v. 151, n. 6, p. 1176-1191, 2016
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/23531
dc.identifier.issn.pt_BR.fl_str_mv	0016-5085
dc.identifier.doi.none.fl_str_mv	10.1053/j.gastro.2016.08.024
identifier_str_mv	DAVID, Bruna Araujo et al. Combination of Mass Cytometry and Imaging Analysis Reveals Origin, Location, and Functional Repopulation of Liver Myeloid Cells in Mice. Gastroenterology. v. 151, n. 6, p. 1176-1191, 2016 0016-5085 10.1053/j.gastro.2016.08.024
url	https://www.arca.fiocruz.br/handle/icict/23531
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Elsevier Inc.
publisher.none.fl_str_mv	Elsevier Inc.
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/23531/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/23531/2/Combination%20of%20Mass%20Cytometry%20and%20Imaging%20Analysis%20Reveals%20Origin%2c%20Location%2c%20and%20Functional%20Repopulation%20of%20Liver%20Myeloid%20Cells%20in%20Mice.pdf https://www.arca.fiocruz.br/bitstream/icict/23531/3/Combination%20of%20Mass%20Cytometry%20and%20Imaging%20Analysis%20Reveals%20Origin%2c%20Location%2c%20and%20Functional%20Repopulation%20of%20Liver%20Myeloid%20Cells%20in%20Mice.pdf.txt
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repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv	repositorio.arca@fiocruz.br
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Combination of Mass Cytometry and Imaging Analysis Reveals Origin, Location, and Functional Repopulation of Liver Myeloid Cells in Mice

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