Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs

Detalhes bibliográficos
Autor(a) principal: Vieira, Yasmine Rangel
Data de Publicação: 2014
Outros Autores: Santos, Debora Regina Lopes dos, Portilho, Moyra Machado, Velloso, Carlos Eduardo Pereira, Arissawa, Marcia, Villar, Livia Melo, Pinto, Marcelo Alves, Paula, Vanessa Salete de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10271
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.
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spelling Vieira, Yasmine RangelSantos, Debora Regina Lopes dosPortilho, Moyra MachadoVelloso, Carlos Eduardo PereiraArissawa, MarciaVillar, Livia MeloPinto, Marcelo AlvesPaula, Vanessa Salete de2015-05-08T14:08:47Z2015-05-08T14:08:47Z2014VIEIRA, Yasmine Rangel et al. Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs. BMC Microbiology, v.14, n.315, 6p, 2014.https://www.arca.fiocruz.br/handle/icict/1027110.1186/s12866-014-0315-2engBiomed CentralHepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Departamento de Mircobiologia Veterinária e Imunologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Bio-Manguinhos. Laboratório de Tecnologia de Anticorpos Monoclonais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Background: Preliminary studies showed the prevalence of a virus similar to human hepatitis B virus (HBV-like) in swine from farms in China and the molecular evidence of Hepadnavirus infection in domestic pigs herds in Brazil. In this study, we genetically characterize the swine Hepadnavirus strains in swine from slaughterhouses located in certified abattoirs from Rio de Janeiro State, Brazil and evaluate its hepatotropic potential. Results: Bile and liver samples from swine were positive for partial genome amplification (ORF S and ORF C), direct sequencing and viral load quantification. Sequencing of the gene encoding the surface antigen allowed classification of Hepadnavirus into genotypes, similar to HBV genotype classification. Indirect immunofluorescence confirmed the presence of HBsAg antigen in liver tissue sections. Conclusions: So far our data suggest that commercial swine house an HBV-like virus and this relevant finding should be considered in studies on the origin and viral evolution.HepadnavirusCommercial swineBrazilBileLiverGenotypingImmunofluorescenceImunofluorescênciaTécnicas de GenotipagemBileFígadoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10271/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALYasmine_vieiraetal_IOC_2014.pdfapplication/pdf2182858https://www.arca.fiocruz.br/bitstream/icict/10271/2/Yasmine_vieiraetal_IOC_2014.pdf5dcdda0a27df5a72bf3a2d5044186d63MD52TEXTYasmine_vieiraetal_IOC_2014.pdf.txtYasmine_vieiraetal_IOC_2014.pdf.txtExtracted texttext/plain24786https://www.arca.fiocruz.br/bitstream/icict/10271/3/Yasmine_vieiraetal_IOC_2014.pdf.txt6f804952bbf8127901b179fc3feaf08dMD53icict/102712023-09-05 10:15:35.878oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-05T13:15:35Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs
title Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs
spellingShingle Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs
Vieira, Yasmine Rangel
Hepadnavirus
Commercial swine
Brazil
Bile
Liver
Genotyping
Immunofluorescence
Imunofluorescência
Técnicas de Genotipagem
Bile
Fígado
title_short Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs
title_full Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs
title_fullStr Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs
title_full_unstemmed Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs
title_sort Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs
author Vieira, Yasmine Rangel
author_facet Vieira, Yasmine Rangel
Santos, Debora Regina Lopes dos
Portilho, Moyra Machado
Velloso, Carlos Eduardo Pereira
Arissawa, Marcia
Villar, Livia Melo
Pinto, Marcelo Alves
Paula, Vanessa Salete de
author_role author
author2 Santos, Debora Regina Lopes dos
Portilho, Moyra Machado
Velloso, Carlos Eduardo Pereira
Arissawa, Marcia
Villar, Livia Melo
Pinto, Marcelo Alves
Paula, Vanessa Salete de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vieira, Yasmine Rangel
Santos, Debora Regina Lopes dos
Portilho, Moyra Machado
Velloso, Carlos Eduardo Pereira
Arissawa, Marcia
Villar, Livia Melo
Pinto, Marcelo Alves
Paula, Vanessa Salete de
dc.subject.en.pt_BR.fl_str_mv Hepadnavirus
Commercial swine
Brazil
Bile
Liver
Genotyping
Immunofluorescence
topic Hepadnavirus
Commercial swine
Brazil
Bile
Liver
Genotyping
Immunofluorescence
Imunofluorescência
Técnicas de Genotipagem
Bile
Fígado
dc.subject.decs.pt_BR.fl_str_mv Imunofluorescência
Técnicas de Genotipagem
Bile
Fígado
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-05-08T14:08:47Z
dc.date.available.fl_str_mv 2015-05-08T14:08:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VIEIRA, Yasmine Rangel et al. Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs. BMC Microbiology, v.14, n.315, 6p, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10271
dc.identifier.doi.pt_BR.fl_str_mv 10.1186/s12866-014-0315-2
identifier_str_mv VIEIRA, Yasmine Rangel et al. Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs. BMC Microbiology, v.14, n.315, 6p, 2014.
10.1186/s12866-014-0315-2
url https://www.arca.fiocruz.br/handle/icict/10271
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Biomed Central
publisher.none.fl_str_mv Biomed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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