Detection of extracellular proteases from microorganisms on agar plates

Detalhes bibliográficos
Autor(a) principal: Vermelho, Alane Beatriz
Data de Publicação: 1996
Outros Autores: Meirelles, Maria Nazareth Leal, Lopes, Andréa, Petinate, Simone Dias Gonçalves, Chaia, André Adriano, Branquinha, Marta Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43814
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Microbiologia Gera. Rio de Janeiro, RJ, Brasil.
id CRUZ_9d5c6ef5b60551c1519b7db666d71765
oai_identifier_str oai:www.arca.fiocruz.br:icict/43814
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Vermelho, Alane BeatrizMeirelles, Maria Nazareth LealLopes, AndréaPetinate, Simone Dias GonçalvesChaia, André AdrianoBranquinha, Marta Helena2020-10-05T17:35:21Z2020-10-05T17:35:21Z1996VERMELHO, Alane Beatriz et al. Detection of extracellular proteases from microorganisms on agar plates. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, RJ, v. 91, n. 6, p. 755-760, Nov./Dec. 1996.0074-0276https://www.arca.fiocruz.br/handle/icict/4381410.1590/S0074-027619960006000201678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.ProteaseDetecçãoMicroorganismoProteaseDetectionMicroorganismDetection of extracellular proteases from microorganisms on agar platesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Microbiologia Gera. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura e Biologia Celular. Rio de Janeiro, GB, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Microbiologia Gera. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Microbiologia Geral. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Microbiologia Geral. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Microbiologia Geral. Rio de Janeiro, RJ, Brasil.We present herein an improved assay for detecting the presence of extracellular proteases from microorganisms on agar plates. Using different substrates (gelatin, BSA, hemoglobin) incorporated into the agar and varying the culture medium composition, we were able to detect proteolytic activities from Pseudomonas aeruginosa, Micrococcus luteus and Serratia marcescens as well as the influence that these components displayed in the expression of these enzymes. For all microorganisms tested we found that in agar-BHI or yeast extract medium containing gelatin the sensitivity of proteinase detection was considerably greater than in BSA-agar or hemoglobin-agar. However, when BSA or hemoglobin were added to the culture medium, there was an increase in growth along with a marked reduction in the amount of proteinase production. In the case of M. luteus the incorporation of glycerol in BHI or yeast extract gelatin-agar induced protease liberation. Our results indicate that the technique described here is of value for detecting extracellular proteases directly in the culture medium, by means of a qualitative assay, simple, inexpensive, straight forward method to assess the presence of the proteolytic activity of a given microorganism colony with great freedom in substrate selection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43814/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMariaNazareLMeireles_AndreaLopes_etal_IOC_1996.pdfMariaNazareLMeireles_AndreaLopes_etal_IOC_1996.pdfapplication/pdf91251https://www.arca.fiocruz.br/bitstream/icict/43814/2/MariaNazareLMeireles_AndreaLopes_etal_IOC_1996.pdf6d0000cbac9495ab577e8e332658f7daMD52TEXTMariaNazareLMeireles_AndreaLopes_etal_IOC_1996.pdf.txtMariaNazareLMeireles_AndreaLopes_etal_IOC_1996.pdf.txtExtracted texttext/plain19091https://www.arca.fiocruz.br/bitstream/icict/43814/3/MariaNazareLMeireles_AndreaLopes_etal_IOC_1996.pdf.txtbdf0c2ed040862e2032b7a5f93aa1d17MD53icict/438142020-10-06 02:04:25.717oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352020-10-06T05:04:25Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Detection of extracellular proteases from microorganisms on agar plates
title Detection of extracellular proteases from microorganisms on agar plates
spellingShingle Detection of extracellular proteases from microorganisms on agar plates
Vermelho, Alane Beatriz
Protease
Detecção
Microorganismo
Protease
Detection
Microorganism
title_short Detection of extracellular proteases from microorganisms on agar plates
title_full Detection of extracellular proteases from microorganisms on agar plates
title_fullStr Detection of extracellular proteases from microorganisms on agar plates
title_full_unstemmed Detection of extracellular proteases from microorganisms on agar plates
title_sort Detection of extracellular proteases from microorganisms on agar plates
author Vermelho, Alane Beatriz
author_facet Vermelho, Alane Beatriz
Meirelles, Maria Nazareth Leal
Lopes, Andréa
Petinate, Simone Dias Gonçalves
Chaia, André Adriano
Branquinha, Marta Helena
author_role author
author2 Meirelles, Maria Nazareth Leal
Lopes, Andréa
Petinate, Simone Dias Gonçalves
Chaia, André Adriano
Branquinha, Marta Helena
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Vermelho, Alane Beatriz
Meirelles, Maria Nazareth Leal
Lopes, Andréa
Petinate, Simone Dias Gonçalves
Chaia, André Adriano
Branquinha, Marta Helena
dc.subject.other.pt_BR.fl_str_mv Protease
Detecção
Microorganismo
topic Protease
Detecção
Microorganismo
Protease
Detection
Microorganism
dc.subject.en.pt_BR.fl_str_mv Protease
Detection
Microorganism
description Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Microbiologia Gera. Rio de Janeiro, RJ, Brasil.
publishDate 1996
dc.date.issued.fl_str_mv 1996
dc.date.accessioned.fl_str_mv 2020-10-05T17:35:21Z
dc.date.available.fl_str_mv 2020-10-05T17:35:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VERMELHO, Alane Beatriz et al. Detection of extracellular proteases from microorganisms on agar plates. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, RJ, v. 91, n. 6, p. 755-760, Nov./Dec. 1996.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43814
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761996000600020
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv VERMELHO, Alane Beatriz et al. Detection of extracellular proteases from microorganisms on agar plates. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, RJ, v. 91, n. 6, p. 755-760, Nov./Dec. 1996.
0074-0276
10.1590/S0074-02761996000600020
1678-8060
url https://www.arca.fiocruz.br/handle/icict/43814
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/43814/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/43814/2/MariaNazareLMeireles_AndreaLopes_etal_IOC_1996.pdf
https://www.arca.fiocruz.br/bitstream/icict/43814/3/MariaNazareLMeireles_AndreaLopes_etal_IOC_1996.pdf.txt
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
6d0000cbac9495ab577e8e332658f7da
bdf0c2ed040862e2032b7a5f93aa1d17
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798325022781079552

Registros relacionados