Inhibition of CAL27 Oral Squamous Carcinoma Cell by Targeting Hedgehog Pathway With Vismodegib or Itraconazole
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/45976 |
Resumo: | FAPESB, grant number APP006/2016 and National Council for Scientific and Technological Development (CNPq) grant number 308595/2016-5. |
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Freitas, Raíza DiasDias, Rosane BorgesVidal, Manuela Torres AndionValverde, Ludmila de FaroCosta, Rafaela Gomes AlvesDamasceno, Andresa Karen AndradeSales, Caroline Brandi SchlaepferRocha, Leonardo de Oliveira Siquara daReis, Mitermayer Galvão dosSoares, Milena Botelho PereiraColetta, Ricardo DellaPereira, Thiago AlmeidaBezerra, Daniel PereiraRocha, Clarissa Araújo Gurgel2021-02-06T13:28:33Z2021-02-06T13:28:33Z2020FREITAS, Raíza Dias et al. Inhibition of CAL27 Oral Squamous Carcinoma Cell by Targeting Hedgehog Pathway With Vismodegib or Itraconazole. Frontiers in Oncology, v. 10, p. 1-12, 2020.2234-943Xhttps://www.arca.fiocruz.br/handle/icict/4597610.3389/fonc.2020.563838FAPESB, grant number APP006/2016 and National Council for Scientific and Technological Development (CNPq) grant number 308595/2016-5.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Department of Pathology and Forensic Medicine. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Dentistry. Department of Propaedeutics. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Department of Pathology and Forensic Medicine. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Department of Pathology and Forensic Medicine. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Federal University of Bahia. Institute of Health Sciences. Department of Biomorphology. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Department of Pathology and Forensic Medicine. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.University of Campinas. School of Dentistry. Department of Oral Diagnostics. Piracicaba, SP, Brazil.Stanford University. Institute for Stem Cell Biology and Regenerative Medicine. Stanford, CA, USA.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Department of Pathology and Forensic Medicine. Salvador, BA, Brazil / Federal University of Bahia. School of Dentistry. Department of Propaedeutics. Salvador, BA, Brazil.Oral Squamous Cell Carcinoma (OSCC) presents an important challenge for the health systems worldwide. Thus, unraveling the biological mechanisms involved in OSCC pathogenesis is essential to the discovery of new drugs with anticancer potential. The Hedgehog (HH) pathway has shown promising results as a therapeutic target both in vitro and in vivo. This study aimed to investigate the effects of vismodegib and itraconazole on the expression of Hedgehog (HH) genes (PTCH1, SMO, and GLI1), cell cycle and cell death in OSCC cells. Alamar Blue assay was used to assess the cytotoxicity of vismodegib and itraconazole in a panel of oral cancer cell lines, including CAL27. The expression of HH signaling components after treatment with vismodegib and itraconazole, at concentrations of 25 or 50 mg/ml was evaluated by qPCR. Cell cycle and apoptosis were evaluated by flow cytometry after 72 h treatment with 50 mg/ml of vismodegib or itraconazole. HH signaling was activated in OSCC cell lines CAL27, SCC4, SCC9, and HSC3. Vismodegib and itraconazole significantly reduced CAL27 cell viability after 48 h of treatment. Gene expression of PTCH1, SMO, and GLI1 decreased in response to 24 h of treatment with vismodegib or itraconazole. Furthermore, CAL27 cells exhibited alterations in morphology, cell size, and cellular granularity. An increase in the DNA fragmentation was observed after treatment and both inhibitors induced apoptosis after 72 h. In conclusion, SMO inhibitors vismodegib and itraconazole demonstrably reduced the expression of HH genes in CAL27 OSCC cell line. In addition, treatment with vismodegib and itraconazole reduced cellular viability and altered the morphology of CAL27 cells, and also induced apoptosis.engFrontiers MediaCarcinoma de células escamosasHedgehogItraconazoleReação em Cadeia da Polimerase em Tempo RealOral squamous cell carcinomaHedgehog pathwayVismodegibItraconazoleReal-time polymerase chain reactionInhibition of CAL27 Oral Squamous Carcinoma Cell by Targeting Hedgehog Pathway With Vismodegib or Itraconazoleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/45976/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALFreitas, Raiza Dias Inhibition of....pdfFreitas, Raiza Dias Inhibition of....pdfapplication/pdf3050503https://www.arca.fiocruz.br/bitstream/icict/45976/2/Freitas%2c%20Raiza%20Dias%20Inhibition%20of....pdf3e82c673748ce30f9660ff66d0ca9a1dMD52TEXTFreitas, Raiza Dias Inhibition of....pdf.txtFreitas, Raiza Dias Inhibition of....pdf.txtExtracted texttext/plain54380https://www.arca.fiocruz.br/bitstream/icict/45976/3/Freitas%2c%20Raiza%20Dias%20Inhibition%20of....pdf.txt85288b1f8610622eb778a6b0a3e90abbMD53icict/459762023-03-15 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| dc.title.pt_BR.fl_str_mv |
Inhibition of CAL27 Oral Squamous Carcinoma Cell by Targeting Hedgehog Pathway With Vismodegib or Itraconazole |
| title |
Inhibition of CAL27 Oral Squamous Carcinoma Cell by Targeting Hedgehog Pathway With Vismodegib or Itraconazole |
| spellingShingle |
Inhibition of CAL27 Oral Squamous Carcinoma Cell by Targeting Hedgehog Pathway With Vismodegib or Itraconazole Freitas, Raíza Dias Carcinoma de células escamosas Hedgehog Itraconazole Reação em Cadeia da Polimerase em Tempo Real Oral squamous cell carcinoma Hedgehog pathway Vismodegib Itraconazole Real-time polymerase chain reaction |
| title_short |
Inhibition of CAL27 Oral Squamous Carcinoma Cell by Targeting Hedgehog Pathway With Vismodegib or Itraconazole |
| title_full |
Inhibition of CAL27 Oral Squamous Carcinoma Cell by Targeting Hedgehog Pathway With Vismodegib or Itraconazole |
| title_fullStr |
Inhibition of CAL27 Oral Squamous Carcinoma Cell by Targeting Hedgehog Pathway With Vismodegib or Itraconazole |
| title_full_unstemmed |
Inhibition of CAL27 Oral Squamous Carcinoma Cell by Targeting Hedgehog Pathway With Vismodegib or Itraconazole |
| title_sort |
Inhibition of CAL27 Oral Squamous Carcinoma Cell by Targeting Hedgehog Pathway With Vismodegib or Itraconazole |
| author |
Freitas, Raíza Dias |
| author_facet |
Freitas, Raíza Dias Dias, Rosane Borges Vidal, Manuela Torres Andion Valverde, Ludmila de Faro Costa, Rafaela Gomes Alves Damasceno, Andresa Karen Andrade Sales, Caroline Brandi Schlaepfer Rocha, Leonardo de Oliveira Siquara da Reis, Mitermayer Galvão dos Soares, Milena Botelho Pereira Coletta, Ricardo Della Pereira, Thiago Almeida Bezerra, Daniel Pereira Rocha, Clarissa Araújo Gurgel |
| author_role |
author |
| author2 |
Dias, Rosane Borges Vidal, Manuela Torres Andion Valverde, Ludmila de Faro Costa, Rafaela Gomes Alves Damasceno, Andresa Karen Andrade Sales, Caroline Brandi Schlaepfer Rocha, Leonardo de Oliveira Siquara da Reis, Mitermayer Galvão dos Soares, Milena Botelho Pereira Coletta, Ricardo Della Pereira, Thiago Almeida Bezerra, Daniel Pereira Rocha, Clarissa Araújo Gurgel |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Freitas, Raíza Dias Dias, Rosane Borges Vidal, Manuela Torres Andion Valverde, Ludmila de Faro Costa, Rafaela Gomes Alves Damasceno, Andresa Karen Andrade Sales, Caroline Brandi Schlaepfer Rocha, Leonardo de Oliveira Siquara da Reis, Mitermayer Galvão dos Soares, Milena Botelho Pereira Coletta, Ricardo Della Pereira, Thiago Almeida Bezerra, Daniel Pereira Rocha, Clarissa Araújo Gurgel |
| dc.subject.other.pt_BR.fl_str_mv |
Carcinoma de células escamosas Hedgehog Itraconazole Reação em Cadeia da Polimerase em Tempo Real |
| topic |
Carcinoma de células escamosas Hedgehog Itraconazole Reação em Cadeia da Polimerase em Tempo Real Oral squamous cell carcinoma Hedgehog pathway Vismodegib Itraconazole Real-time polymerase chain reaction |
| dc.subject.en.pt_BR.fl_str_mv |
Oral squamous cell carcinoma Hedgehog pathway Vismodegib Itraconazole Real-time polymerase chain reaction |
| description |
FAPESB, grant number APP006/2016 and National Council for Scientific and Technological Development (CNPq) grant number 308595/2016-5. |
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2020 |
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2020 |
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2021-02-06T13:28:33Z |
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2021-02-06T13:28:33Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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FREITAS, Raíza Dias et al. Inhibition of CAL27 Oral Squamous Carcinoma Cell by Targeting Hedgehog Pathway With Vismodegib or Itraconazole. Frontiers in Oncology, v. 10, p. 1-12, 2020. |
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https://www.arca.fiocruz.br/handle/icict/45976 |
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2234-943X |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fonc.2020.563838 |
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FREITAS, Raíza Dias et al. Inhibition of CAL27 Oral Squamous Carcinoma Cell by Targeting Hedgehog Pathway With Vismodegib or Itraconazole. Frontiers in Oncology, v. 10, p. 1-12, 2020. 2234-943X 10.3389/fonc.2020.563838 |
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Frontiers Media |
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Frontiers Media |
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