Non-culture based diagnostic tests for mycotic infections

Detalhes bibliográficos
Autor(a) principal: Reiss, E.
Data de Publicação: 2000
Outros Autores: Obayashi, T., Orle, K., Yoshida, M., Zancopé-Oliveira, R. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33535
Resumo: 2020-06-18
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spelling Reiss, E.Obayashi, T.Orle, K.Yoshida, M.Zancopé-Oliveira, R. M.2019-06-18T12:30:45Z2019-06-18T12:30:45Z2000REISS, E. et al. Non-culture based diagnostic tests for mycotic infections. Medical Mycology, v. 38, n. 1, p. 147-159, 2000.1369-3786https://www.arca.fiocruz.br/handle/icict/3353510.1080/mmy.38.s1.147.1591460-2709engOxford University PressNon-culture based diagnostic tests for mycotic infectionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-06-18Centers for Disease Control and Prevention. Mycotic Diseases Branch. Atlanta, GA, USA.Jichi Medical School. Department of Clinical Pathology. Tochigi-ken, Japan.Roche Molecular Diagnostics. Division of Infectious Disease. Alameda, CA, USA.Teikyo University School of Medicine. Fourth Department of Internal Medicine. Kawasaki City, Japan.Fundação Oswaldo Cruz. Hospital Evandro Chagas. Centro de Pesquisas. Laboratório de Micologia Médica. Rio de Janeiro, RJ, Brasil.Non-culture methods being developed and evaluated for mycotic infections include polymerase chain reaction (PCR), galactomannan (GM) antigenemia, Western blot (WB) to detect antibodies, and detection of the fungal metabolites D-arabinitol and (1,3)-b-D-glucan. Sample preparation for PCR from blood specimens depends on fractionation of peripheral blood, its pre-incubation in blood culture broth, or a total DNA method, which does not rely on fractionation, or pre-incubation. Targets for PCR of fungi in the 18S or ITS2 subunits of the ribosomal RNA genes facilitated the design of Aspergillus and Candida genus and species probes. Amplicons were identi ed using PCR-enzyme linked immunosorbent assay (ELISA) or reverse lineblot formats. A pilot study indicated that PCR tests on blood specimens were positive at least once in patients with con rmed invasive aspergillosis (IA). When serum-PCR and serum-GM tests were compared in IA patients, antigenemia was more often positive. PCR detected Aspergillus DNA in bronchoalveolar lavage specimens from patients at risk even when cultures were negative. D-Arabinitol can be detected as a marker of candidiasis with gas chromatography-mass spectrometry or enzyme dependent- uorometry. Each method can differentiate the microbial D- and host L-enantiomers. (1,3)-b-D-Glucan is produced by most genera of pathogenic fungi and can be detected in plasma by the ‘G-test’. In patients with febrile neutropenia the ef cacy of azole therapy correlated with plasma (1,3)-b-D-glucan concentrations of ]10 pg ml¼1. The diagnosis of early acute pulmonary histoplasmosis can be improved by a WB test utilizing deglycosylated M antigen, a 94-kDa glycoprotein. The identity of M antigen as a catalase was deduced from the sequence of the cloned gene. PCR identi cation of Histoplasma capsulatum cultures was accomplished with primer pairs selected from H and M antigen gene sequences.Fungal PCRGalactomannanHistoplasmaM antigeninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/33535/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Reiss_E_etal_INI_2000.pdfve_Reiss_E_etal_INI_2000.pdfapplication/pdf279268https://www.arca.fiocruz.br/bitstream/icict/33535/2/ve_Reiss_E_etal_INI_2000.pdfa08261f72919b04fbf317089e094553cMD52TEXTve_Reiss_E_etal_INI_2000.pdf.txtve_Reiss_E_etal_INI_2000.pdf.txtExtracted texttext/plain71356https://www.arca.fiocruz.br/bitstream/icict/33535/3/ve_Reiss_E_etal_INI_2000.pdf.txtd6a6bca57c764b729bc730d07638c9b8MD53icict/335352021-03-24 16:32:33.119oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:33Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Non-culture based diagnostic tests for mycotic infections
title Non-culture based diagnostic tests for mycotic infections
spellingShingle Non-culture based diagnostic tests for mycotic infections
Reiss, E.
Fungal PCR
Galactomannan
Histoplasma
M antigen
title_short Non-culture based diagnostic tests for mycotic infections
title_full Non-culture based diagnostic tests for mycotic infections
title_fullStr Non-culture based diagnostic tests for mycotic infections
title_full_unstemmed Non-culture based diagnostic tests for mycotic infections
title_sort Non-culture based diagnostic tests for mycotic infections
author Reiss, E.
author_facet Reiss, E.
Obayashi, T.
Orle, K.
Yoshida, M.
Zancopé-Oliveira, R. M.
author_role author
author2 Obayashi, T.
Orle, K.
Yoshida, M.
Zancopé-Oliveira, R. M.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Reiss, E.
Obayashi, T.
Orle, K.
Yoshida, M.
Zancopé-Oliveira, R. M.
dc.subject.en.pt_BR.fl_str_mv Fungal PCR
Galactomannan
Histoplasma
M antigen
topic Fungal PCR
Galactomannan
Histoplasma
M antigen
description 2020-06-18
publishDate 2000
dc.date.issued.fl_str_mv 2000
dc.date.accessioned.fl_str_mv 2019-06-18T12:30:45Z
dc.date.available.fl_str_mv 2019-06-18T12:30:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv REISS, E. et al. Non-culture based diagnostic tests for mycotic infections. Medical Mycology, v. 38, n. 1, p. 147-159, 2000.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33535
dc.identifier.issn.pt_BR.fl_str_mv 1369-3786
dc.identifier.doi.none.fl_str_mv 10.1080/mmy.38.s1.147.159
dc.identifier.eissn.none.fl_str_mv 1460-2709
identifier_str_mv REISS, E. et al. Non-culture based diagnostic tests for mycotic infections. Medical Mycology, v. 38, n. 1, p. 147-159, 2000.
1369-3786
10.1080/mmy.38.s1.147.159
1460-2709
url https://www.arca.fiocruz.br/handle/icict/33535
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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