Trypanosoma cruzi TcSMUG L-surface Mucins Promote Development and Infectivity in the Triatomine Vector Rhodnius prolixus
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/11553 |
Resumo: | Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil / Instituto Nacional de Entomologia Molecular (INCT-EM, CNPq). Brasil. |
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Gonzalez, Marcelo S.Souza, Marcela S.Garcia, Eloi S.Nogueira, Nadir F. S.Mello, Cicero B.Cánepa, Gaspar E.Bertotti, SantiagoDurante, Ignacio M.Azambuja, PatríciaBuscaglia, Carlos A,2015-08-19T13:49:31Z2015-08-19T13:49:31Z2013GONZALEZ, Marcelo S. Trypanosoma cruzi TcSMUG L-surface Mucins Promote Development and Infectivity in the Triatomine Vector Rhodnius prolixus. PLoS Negl Trop Dis., v.7, n.11, e2552, 10p, nov. 2013.https://www.arca.fiocruz.br/handle/icict/1155310.1371/journal.pntd.0002552engPlos OneTrypanosoma cruzi TcSMUG L-surface Mucins Promote Development and Infectivity in the Triatomine Vector Rhodnius prolixusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil / Instituto Nacional de Entomologia Molecular (INCT-EM, CNPq). Brasil.Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil.Instituto Nacional de Entomologia Molecular (INCT-EM, CNPq). Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Universidade Estadual do Norte Fluminense - Horto. Centro de Biocieˆncias e Biotecnologia. Laborato´ rio de Biologia Celular e Tecidual. Campos dos Goytacases, RJ, Brasil.Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil / Instituto Nacional de Entomologia Molecular (INCT-EM, CNPq). Brasil.Universidad Nacional de San Martín (UNSAM) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Biotecnológicas ‘‘Dr Rodolfo Ugalde’’. Campus UNSAM. Buenos Aires, Argentina.Universidad Nacional de San Martín (UNSAM) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Biotecnológicas ‘‘Dr Rodolfo Ugalde’’. Campus UNSAM. Buenos Aires, Argentina.nstituto Nacional de Entomologia Molecular (INCT-EM, CNPq). Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Universidad Nacional de San Martín (UNSAM) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Biotecnológicas ‘‘Dr Rodolfo Ugalde’’. Campus UNSAM. Buenos Aires, Argentina.Background: TcSMUG L products were recently identified as novel mucin-type glycoconjugates restricted to the surface of insect-dwelling epimastigote forms of Trypanosoma cruzi, the etiological agent of Chagas disease. The remarkable conservation of their predicted mature N-terminal region, which is exposed to the extracellular milieu, suggests that TcSMUG L products may be involved in structural and/or functional aspects of the interaction with the insect vector. Methodology and Principal Findings: Here, we investigated the putative roles of TcSMUG L mucins in both in vivo development and ex vivo attachment of epimastigotes to the luminal surface of the digestive tract of Rhodnius prolixus. Our results indicate that the exogenous addition of TcSMUG L N-terminal peptide, but not control T. cruzi mucin peptides, to the infected bloodmeal inhibited the development of parasites in R. prolixus in a dose-dependent manner. Pre-incubation of insect midguts with the TcSMUG L peptide impaired the ex vivo attachment of epimastigotes to the luminal surface epithelium, likely by competing out TcSMUG L binding sites on the luminal surface of the posterior midgut, as revealed by fluorescence microscopy. Conclusion and Significance: Together, these observations indicate that TcSMUG L mucins are a determinant of both adhesion of T. cruzto the posterior midgut epithelial cells of the triatomine, and the infection of the insect vector, R. prolixus.Rhodnius prolixusTrypanosoma cruziTriatomine VectorMucinsTrypanosoma cruziTriatominaeRhodniusMucinasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11553/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALeloi_garcia_etal_IOC_2013.pdfapplication/pdf6467561https://www.arca.fiocruz.br/bitstream/icict/11553/2/eloi_garcia_etal_IOC_2013.pdf3d08464f54865b8fee87e5456fed719cMD52TEXTeloi_garcia_etal_IOC_2013.pdf.txteloi_garcia_etal_IOC_2013.pdf.txtExtracted texttext/plain51455https://www.arca.fiocruz.br/bitstream/icict/11553/3/eloi_garcia_etal_IOC_2013.pdf.txt9ef288bc8c2d708b15feb3495a190b7bMD53icict/115532022-06-24 12:18:55.446oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:18:55Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Trypanosoma cruzi TcSMUG L-surface Mucins Promote Development and Infectivity in the Triatomine Vector Rhodnius prolixus |
| title |
Trypanosoma cruzi TcSMUG L-surface Mucins Promote Development and Infectivity in the Triatomine Vector Rhodnius prolixus |
| spellingShingle |
Trypanosoma cruzi TcSMUG L-surface Mucins Promote Development and Infectivity in the Triatomine Vector Rhodnius prolixus Gonzalez, Marcelo S. Rhodnius prolixus Trypanosoma cruzi Triatomine Vector Mucins Trypanosoma cruzi Triatominae Rhodnius Mucinas |
| title_short |
Trypanosoma cruzi TcSMUG L-surface Mucins Promote Development and Infectivity in the Triatomine Vector Rhodnius prolixus |
| title_full |
Trypanosoma cruzi TcSMUG L-surface Mucins Promote Development and Infectivity in the Triatomine Vector Rhodnius prolixus |
| title_fullStr |
Trypanosoma cruzi TcSMUG L-surface Mucins Promote Development and Infectivity in the Triatomine Vector Rhodnius prolixus |
| title_full_unstemmed |
Trypanosoma cruzi TcSMUG L-surface Mucins Promote Development and Infectivity in the Triatomine Vector Rhodnius prolixus |
| title_sort |
Trypanosoma cruzi TcSMUG L-surface Mucins Promote Development and Infectivity in the Triatomine Vector Rhodnius prolixus |
| author |
Gonzalez, Marcelo S. |
| author_facet |
Gonzalez, Marcelo S. Souza, Marcela S. Garcia, Eloi S. Nogueira, Nadir F. S. Mello, Cicero B. Cánepa, Gaspar E. Bertotti, Santiago Durante, Ignacio M. Azambuja, Patrícia Buscaglia, Carlos A, |
| author_role |
author |
| author2 |
Souza, Marcela S. Garcia, Eloi S. Nogueira, Nadir F. S. Mello, Cicero B. Cánepa, Gaspar E. Bertotti, Santiago Durante, Ignacio M. Azambuja, Patrícia Buscaglia, Carlos A, |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Gonzalez, Marcelo S. Souza, Marcela S. Garcia, Eloi S. Nogueira, Nadir F. S. Mello, Cicero B. Cánepa, Gaspar E. Bertotti, Santiago Durante, Ignacio M. Azambuja, Patrícia Buscaglia, Carlos A, |
| dc.subject.en.pt_BR.fl_str_mv |
Rhodnius prolixus Trypanosoma cruzi Triatomine Vector Mucins |
| topic |
Rhodnius prolixus Trypanosoma cruzi Triatomine Vector Mucins Trypanosoma cruzi Triatominae Rhodnius Mucinas |
| dc.subject.decs.pt_BR.fl_str_mv |
Trypanosoma cruzi Triatominae Rhodnius Mucinas |
| description |
Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil / Instituto Nacional de Entomologia Molecular (INCT-EM, CNPq). Brasil. |
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2013 |
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2013 |
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2015-08-19T13:49:31Z |
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2015-08-19T13:49:31Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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GONZALEZ, Marcelo S. Trypanosoma cruzi TcSMUG L-surface Mucins Promote Development and Infectivity in the Triatomine Vector Rhodnius prolixus. PLoS Negl Trop Dis., v.7, n.11, e2552, 10p, nov. 2013. |
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https://www.arca.fiocruz.br/handle/icict/11553 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1371/journal.pntd.0002552 |
| identifier_str_mv |
GONZALEZ, Marcelo S. Trypanosoma cruzi TcSMUG L-surface Mucins Promote Development and Infectivity in the Triatomine Vector Rhodnius prolixus. PLoS Negl Trop Dis., v.7, n.11, e2552, 10p, nov. 2013. 10.1371/journal.pntd.0002552 |
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https://www.arca.fiocruz.br/handle/icict/11553 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Plos One |
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Plos One |
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