Uptake of Host Cell Transforming Growth Factor- by Trypanosoma cruzi Amastigotes in Cardiomyocytes Potential Role in Parasite Cycle Completion
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2005 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/40960 |
Resumo: | Acesso aberto em 24/04/2020 - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1603686/pdf/JPATH167000993.pdf |
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Waghabi, Mariana C.Keramidas, MichelleBaily, SabineDegrave, Wim M.LIma, Leila MendonçaSoeiro, Maria Nazaré C.Meirelles, Maria de Nazareth L.Paciornik, SidneyAraujo-Jorge, Tania C.Feige, Jean-Jacques2020-04-24T17:58:39Z2020-04-24T17:58:39Z2005WAGHABI, Mariana C. et al. Uptake of Host Cell Transforming Growth Factor-β by Trypanosoma cruzi Amastigotes in Cardiomyocytes Potential Role in Parasite Cycle Completion. The American Journal of Pathology, v. 167, n. 4, p. 993-1003, 2005.0002-9440https://www.arca.fiocruz.br/handle/icict/4096010.1016/s0002-9440(10)61189-31525-2191Acesso aberto em 24/04/2020 - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1603686/pdf/JPATH167000993.pdfFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Commissariat à l’Energie Atomique.Grenoble, France.Commissariat à l’Energie Atomique.Grenoble, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular..Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil.Pontifícia Universidade Católica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Commissariat à l’Énergie Atomique, Grenoble, FranceThe cytokine transforming growth factor- (TGF- ) plays various functions in the control of Trypanosoma cruzi infectivity and in the progression of Chagas’ disease. When we immunostained T. cruzi-infected cardiomyocytes (after either in vivo or in vitro infections) for TGF- , we observed stronger immunoreactivity in parasites than in host cells. TGF- immunoreactivity evolved during parasite cycle progression, with intense staining in amastigotes versus very faint staining in trypomastigotes. TGF- was present on the surface of amastigotes, in the flagellar pocket, and in intraparasitic vesicles as revealed by electron microscopy. However, no ortholog TGF- gene could be identified in the genome of T. cruzi by in silico analysis or by extensive polymerase chain reaction and reverse transcriptase-polymerase chain reaction studies. Immunoreactive TGF- was most probably taken up by the parasite from the host cell cytoplasm because such an internalization process of biotinylated TGF- could be observed in axenic amastigotes in vitro. These observations represent the first example of a novel mechanism by which a primitive unicellular protozoan can use host cell TGF- to control its own intracellular life cycle.engAmerican Society for Investigative PathologyFator de crescimento de células hospedeirasTrypanosoma cruziAmastigotesCardiomiócitosHost cell transforming Growth FactorTrypanosoma cruziAmastigotesCardiomyocytesUptake of Host Cell Transforming Growth Factor- by Trypanosoma cruzi Amastigotes in Cardiomyocytes Potential Role in Parasite Cycle Completioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Uptake of Host Cell Transforming Growth Factor- by Trypanosoma cruzi Amastigotes in Cardiomyocytes Potential Role in Parasite Cycle Completion |
| title |
Uptake of Host Cell Transforming Growth Factor- by Trypanosoma cruzi Amastigotes in Cardiomyocytes Potential Role in Parasite Cycle Completion |
| spellingShingle |
Uptake of Host Cell Transforming Growth Factor- by Trypanosoma cruzi Amastigotes in Cardiomyocytes Potential Role in Parasite Cycle Completion Waghabi, Mariana C. Fator de crescimento de células hospedeiras Trypanosoma cruzi Amastigotes Cardiomiócitos Host cell transforming Growth Factor Trypanosoma cruzi Amastigotes Cardiomyocytes |
| title_short |
Uptake of Host Cell Transforming Growth Factor- by Trypanosoma cruzi Amastigotes in Cardiomyocytes Potential Role in Parasite Cycle Completion |
| title_full |
Uptake of Host Cell Transforming Growth Factor- by Trypanosoma cruzi Amastigotes in Cardiomyocytes Potential Role in Parasite Cycle Completion |
| title_fullStr |
Uptake of Host Cell Transforming Growth Factor- by Trypanosoma cruzi Amastigotes in Cardiomyocytes Potential Role in Parasite Cycle Completion |
| title_full_unstemmed |
Uptake of Host Cell Transforming Growth Factor- by Trypanosoma cruzi Amastigotes in Cardiomyocytes Potential Role in Parasite Cycle Completion |
| title_sort |
Uptake of Host Cell Transforming Growth Factor- by Trypanosoma cruzi Amastigotes in Cardiomyocytes Potential Role in Parasite Cycle Completion |
| author |
Waghabi, Mariana C. |
| author_facet |
Waghabi, Mariana C. Keramidas, Michelle Baily, Sabine Degrave, Wim M. LIma, Leila Mendonça Soeiro, Maria Nazaré C. Meirelles, Maria de Nazareth L. Paciornik, Sidney Araujo-Jorge, Tania C. Feige, Jean-Jacques |
| author_role |
author |
| author2 |
Keramidas, Michelle Baily, Sabine Degrave, Wim M. LIma, Leila Mendonça Soeiro, Maria Nazaré C. Meirelles, Maria de Nazareth L. Paciornik, Sidney Araujo-Jorge, Tania C. Feige, Jean-Jacques |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Waghabi, Mariana C. Keramidas, Michelle Baily, Sabine Degrave, Wim M. LIma, Leila Mendonça Soeiro, Maria Nazaré C. Meirelles, Maria de Nazareth L. Paciornik, Sidney Araujo-Jorge, Tania C. Feige, Jean-Jacques |
| dc.subject.other.pt_BR.fl_str_mv |
Fator de crescimento de células hospedeiras Trypanosoma cruzi Amastigotes Cardiomiócitos |
| topic |
Fator de crescimento de células hospedeiras Trypanosoma cruzi Amastigotes Cardiomiócitos Host cell transforming Growth Factor Trypanosoma cruzi Amastigotes Cardiomyocytes |
| dc.subject.en.pt_BR.fl_str_mv |
Host cell transforming Growth Factor Trypanosoma cruzi Amastigotes Cardiomyocytes |
| description |
Acesso aberto em 24/04/2020 - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1603686/pdf/JPATH167000993.pdf |
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2005 |
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2005 |
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2020-04-24T17:58:39Z |
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2020-04-24T17:58:39Z |
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WAGHABI, Mariana C. et al. Uptake of Host Cell Transforming Growth Factor-β by Trypanosoma cruzi Amastigotes in Cardiomyocytes Potential Role in Parasite Cycle Completion. The American Journal of Pathology, v. 167, n. 4, p. 993-1003, 2005. |
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https://www.arca.fiocruz.br/handle/icict/40960 |
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0002-9440 |
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10.1016/s0002-9440(10)61189-3 |
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1525-2191 |
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WAGHABI, Mariana C. et al. Uptake of Host Cell Transforming Growth Factor-β by Trypanosoma cruzi Amastigotes in Cardiomyocytes Potential Role in Parasite Cycle Completion. The American Journal of Pathology, v. 167, n. 4, p. 993-1003, 2005. 0002-9440 10.1016/s0002-9440(10)61189-3 1525-2191 |
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American Society for Investigative Pathology |
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American Society for Investigative Pathology |
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