Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds

Detalhes bibliográficos
Autor(a) principal: Soares, Emanoella L.
Data de Publicação: 2014
Outros Autores: Shah, Mohibullah, Soares, Arlete A., Costa, José H., Carvalho, Paulo Costa, Domont, Gilberto B., Nogueira, Fábio Cesar Sousa, Campos, Francisco A. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8942
Resumo: 2015-07-31
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spelling Soares, Emanoella L.Shah, MohibullahSoares, Arlete A.Costa, José H.Carvalho, Paulo CostaDomont, Gilberto B.Nogueira, Fábio Cesar SousaCampos, Francisco A. P.2014-11-24T12:13:37Z2015-08-01T07:30:05Z2014SOARES, Emanoella L. et al. Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds. Journal of Proteome Research, v. 13, n. 8, p. 3562-3570, 2014.1535-3893https://www.arca.fiocruz.br/handle/icict/894210.1021/pr5004505engJournal of Proteome ResearchProteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seedsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2015-07-31Federal University of Ceara. Department of Biochemistry and Molecular Biology. Fortaleza, CE, Brazil.Federal University of Ceara. Department of Biochemistry and Molecular Biology. Fortaleza, CE, Brazil.Federal University of Ceara. Department of Biology. Campus do Pici, Fortaleza, CE, Brazil.Federal University of Ceara. Department of Biochemistry and Molecular Biology. Fortaleza, CE, Brazil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil.Federal University of Rio de Janeiro. Proteomic Unit, Institute of Chemistry. Rio de Janeiro, RJ, Brazil.Federal University of Rio de Janeiro. Proteomic Unit, Institute of Chemistry. Rio de Janeiro, RJ, Brazil.Federal University of Ceara. Department of Biochemistry and Molecular Biology. Fortaleza, CE, Brazil.In this study, we performed a systematic proteomic analysis of the inner integument from developing seeds of Jatropha curcas and further explored the protein machinery responsible for generating the carbon and nitrogen sources to feed the growing embryo and endosperm. The inner integument of developing seeds was dissected into two sections called distal and proximal, and proteins were extracted from these sections and from the whole integument and analyzed using an EASY-nanoLC system coupled to an ESILTQ-Orbitrap Velos mass spectrometer. We identified 1526, 1192, and 1062 proteins from the proximal, distal, and whole inner integuments, respectively. The identifications include those of peptidases and other hydrolytic enzymes that play a key role in developmental programmed cell death and proteins associated with the cell-wall architecture and modification. Because many of these proteins are differentially expressed within the integument cell layers, these findings suggest that the cells mobilize an array of hydrolases to produce carbon and nitrogen sources from proteins, carbohydrates, and lipids available within the cells. Not least, the identification of several classes of seed storage proteins in the inner integument provides additional evidence of the role of the seed coat as a transient source of reserves for the growing embryo and endosperm.Label-free quantitative proteomicsProgrammed cell deathJatropha curcasOilseedSeed coatinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALartigo1.pdfartigo1.pdfapplication/pdf5207876https://www.arca.fiocruz.br/bitstream/icict/8942/2/artigo1.pdf11e8e1f47f0046ef1b5b476cde62a038MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8942/3/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD53TEXTartigo1.pdf.txtartigo1.pdf.txtExtracted texttext/plain51931https://www.arca.fiocruz.br/bitstream/icict/8942/4/artigo1.pdf.txt3baa29afcc0d222f1eb930deda44a601MD54icict/89422021-03-24 16:32:18.184oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:18Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds
title Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds
spellingShingle Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds
Soares, Emanoella L.
Label-free quantitative proteomics
Programmed cell death
Jatropha curcas
Oilseed
Seed coat
title_short Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds
title_full Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds
title_fullStr Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds
title_full_unstemmed Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds
title_sort Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds
author Soares, Emanoella L.
author_facet Soares, Emanoella L.
Shah, Mohibullah
Soares, Arlete A.
Costa, José H.
Carvalho, Paulo Costa
Domont, Gilberto B.
Nogueira, Fábio Cesar Sousa
Campos, Francisco A. P.
author_role author
author2 Shah, Mohibullah
Soares, Arlete A.
Costa, José H.
Carvalho, Paulo Costa
Domont, Gilberto B.
Nogueira, Fábio Cesar Sousa
Campos, Francisco A. P.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Soares, Emanoella L.
Shah, Mohibullah
Soares, Arlete A.
Costa, José H.
Carvalho, Paulo Costa
Domont, Gilberto B.
Nogueira, Fábio Cesar Sousa
Campos, Francisco A. P.
dc.subject.en.pt_BR.fl_str_mv Label-free quantitative proteomics
Programmed cell death
Jatropha curcas
Oilseed
Seed coat
topic Label-free quantitative proteomics
Programmed cell death
Jatropha curcas
Oilseed
Seed coat
description 2015-07-31
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-11-24T12:13:37Z
dc.date.issued.fl_str_mv 2014
dc.date.available.fl_str_mv 2015-08-01T07:30:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOARES, Emanoella L. et al. Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds. Journal of Proteome Research, v. 13, n. 8, p. 3562-3570, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8942
dc.identifier.issn.none.fl_str_mv 1535-3893
dc.identifier.doi.pt_BR.fl_str_mv 10.1021/pr5004505
identifier_str_mv SOARES, Emanoella L. et al. Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds. Journal of Proteome Research, v. 13, n. 8, p. 3562-3570, 2014.
1535-3893
10.1021/pr5004505
url https://www.arca.fiocruz.br/handle/icict/8942
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Journal of Proteome Research
publisher.none.fl_str_mv Journal of Proteome Research
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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