New Approaches with Different Types of Circulating Cathodic Antigen for the Diagnosis of Patients with Low Schistosoma mansoni Load

Detalhes bibliográficos
Autor(a) principal: Queiroz, Rafaella Fortini Grenfell e
Data de Publicação: 2013
Outros Autores: Harn, Donald A., Tundup, Smanla, Da'dara, Akram, Siqueira, Liliane, Coelho, Paulo Marcos Zech
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/59322
Resumo: Schistosomiasis Laboratory. Rene Rachou Research Center. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/Department of Infectious Diseases. College of Veterinary Medicine and the Center for Tropical and Emerging Diseases. University of Georgia. Athens, GA, United States of America
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spelling Queiroz, Rafaella Fortini Grenfell eHarn, Donald A.Tundup, SmanlaDa'dara, AkramSiqueira, LilianeCoelho, Paulo Marcos Zech2023-06-30T17:54:37Z2023-06-30T17:54:37Z2013QUEIROZ, Rafaella Fortini Grenfell e et al. New approaches with different types of circulating cathodic antigen for the diagnosis of patients with low Schistosoma mansoni load. PLoS Negl Trop Dis., v. 7, n. 2, e2054, 2013. doi: 10.1371/journal.pntd.0002054.1935-2735https://www.arca.fiocruz.br/handle/icict/5932210.1371/journal.pntd.0002054engPublic Library of ScienceNew Approaches with Different Types of Circulating Cathodic Antigen for the Diagnosis of Patients with Low Schistosoma mansoni Loadinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSchistosomiasis Laboratory. Rene Rachou Research Center. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/Department of Infectious Diseases. College of Veterinary Medicine and the Center for Tropical and Emerging Diseases. University of Georgia. Athens, GA, United States of AmericaDepartment of Infectious Diseases. College of Veterinary Medicine and the Center for Tropical and Emerging Diseases. University of Georgia. Athens, GA, United States of AmericaDepartment of Infectious Diseases. College of Veterinary Medicine and the Center for Tropical and Emerging Diseases. University of Georgia. Athens, GA, United States of AmericaTufts University. Grafton, MA, United States of America/Centers for Disease Control and Prevention. United States of AmericaSchistosomiasis Laboratory. Rene Rachou Research Center. Oswaldo Cruz Foundation. Belo Horizonte, MG, BrazilSchistosomiasis Laboratory. Rene Rachou Research Center. Oswaldo Cruz Foundation. Belo Horizonte, MG, BrazilBackground: Schistosomiasis mansoni is a debilitating and sometimes fatal disease. Accurate diagnosis plays a key role in patient management and infection control. However, currently available parasitological methods are laborious and lack sensitivity. The selection of target antigen candidates has turned out to be a promising tool for the development of more sensitive diagnostic methods. In our previous investigations, the use of crude antigens led to false-positive results. Recently, focus has been given to highly purified Schistosoma mansoni antigens, especially to circulating antigens. Method: Thus, our main goal was to test different types of circulating cathodic antigen glycoprotein (CCA), as "crude antigen," the protein chain of recombinant CCA and two individual peptides. These schistosome proteins/peptides were tested in a new diagnostic method employing immunomagnetic separation based on the improvement of antigen-antibody binding. Principal Findings: Use of recombinant CCA as a diagnostic antigen allowed us to develop a diagnostic assay with high sensitivity and specificity with no false-negative results. Interestingly, the "crude antigen" worked as a good marker for control of cure after praziquantel treatment. Conclusions/Significance: Our new diagnostic method was superior to enzyme-linked immunosorbent assay in diagnosing low endemicity patientsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALpntd.0002054 1..10.pdfpntd.0002054 1..10.pdfapplication/pdf718902https://www.arca.fiocruz.br/bitstream/icict/59322/2/pntd.0002054%201..10.pdf44ae734e4900cb3d0a697ee82e3ff522MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/59322/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/593222023-06-30 14:54:38.873oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-06-30T17:54:38Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv New Approaches with Different Types of Circulating Cathodic Antigen for the Diagnosis of Patients with Low Schistosoma mansoni Load
title New Approaches with Different Types of Circulating Cathodic Antigen for the Diagnosis of Patients with Low Schistosoma mansoni Load
spellingShingle New Approaches with Different Types of Circulating Cathodic Antigen for the Diagnosis of Patients with Low Schistosoma mansoni Load
Queiroz, Rafaella Fortini Grenfell e
title_short New Approaches with Different Types of Circulating Cathodic Antigen for the Diagnosis of Patients with Low Schistosoma mansoni Load
title_full New Approaches with Different Types of Circulating Cathodic Antigen for the Diagnosis of Patients with Low Schistosoma mansoni Load
title_fullStr New Approaches with Different Types of Circulating Cathodic Antigen for the Diagnosis of Patients with Low Schistosoma mansoni Load
title_full_unstemmed New Approaches with Different Types of Circulating Cathodic Antigen for the Diagnosis of Patients with Low Schistosoma mansoni Load
title_sort New Approaches with Different Types of Circulating Cathodic Antigen for the Diagnosis of Patients with Low Schistosoma mansoni Load
author Queiroz, Rafaella Fortini Grenfell e
author_facet Queiroz, Rafaella Fortini Grenfell e
Harn, Donald A.
Tundup, Smanla
Da'dara, Akram
Siqueira, Liliane
Coelho, Paulo Marcos Zech
author_role author
author2 Harn, Donald A.
Tundup, Smanla
Da'dara, Akram
Siqueira, Liliane
Coelho, Paulo Marcos Zech
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Queiroz, Rafaella Fortini Grenfell e
Harn, Donald A.
Tundup, Smanla
Da'dara, Akram
Siqueira, Liliane
Coelho, Paulo Marcos Zech
description Schistosomiasis Laboratory. Rene Rachou Research Center. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/Department of Infectious Diseases. College of Veterinary Medicine and the Center for Tropical and Emerging Diseases. University of Georgia. Athens, GA, United States of America
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2023-06-30T17:54:37Z
dc.date.available.fl_str_mv 2023-06-30T17:54:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv QUEIROZ, Rafaella Fortini Grenfell e et al. New approaches with different types of circulating cathodic antigen for the diagnosis of patients with low Schistosoma mansoni load. PLoS Negl Trop Dis., v. 7, n. 2, e2054, 2013. doi: 10.1371/journal.pntd.0002054.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59322
dc.identifier.issn.en_US.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0002054
identifier_str_mv QUEIROZ, Rafaella Fortini Grenfell e et al. New approaches with different types of circulating cathodic antigen for the diagnosis of patients with low Schistosoma mansoni load. PLoS Negl Trop Dis., v. 7, n. 2, e2054, 2013. doi: 10.1371/journal.pntd.0002054.
1935-2735
10.1371/journal.pntd.0002054
url https://www.arca.fiocruz.br/handle/icict/59322
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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collection Repositório Institucional da FIOCRUZ (ARCA)
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